Decellularized Cartilage Matrix as a Novel Biomatrix for Cartilage Tissue-Engineering Applications

Schwarz, Silke; Koerber, Ludwig; Elsaesser, Alexander F.; Goldberg‐Bockhorn, Eva; Seitz, Alexander; Dürselen, Lutz; Ignatius, Anita; Walther, Paul; Breiter, Roman; Rotter, Nicole

doi:10.1089/ten.tea.2011.0705

Cited by 209 publications

(261 citation statements)

References 70 publications

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“…However the decellularisation process also increased the amount of denatured collagen compared with native cartilage. Overall there was significant decrease of biomechanical loading stress, which the acellular matrix showing reduced stiffness by about 69.5% [146]. The matrix did however support the growth of chondrocytes and re-acculumation of proteoglycans in the process of in vitro culture [147].…”

Section: Acellular Biological Scaffoldsmentioning

confidence: 89%

Bioengineering of Articular Cartilage: Past, Present and Future

Felimban

Moulton

et al. 2013

Regen. Med.

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The treatment of cartilage defects poses a clinical challenge owing to the lack of intrinsic regenerative capacity of cartilage. The use of tissue engineering techniques to bioengineer articular cartilage is promising and may hold the key to the successful regeneration of cartilage tissue. Natural and synthetic biomaterials have been used to recreate the microarchitecture of articular cartilage through multilayered biomimetic scaffolds. Acellular scaffolds preserve the microarchitecture of articular cartilage through a process of decellularization of biological tissue. Although promising, this technique often results in poor biomechanical strength of the graft. However, biomechanical strength could be improved if biomaterials could be incorporated back into the decellularized tissue to overcome this limitation.

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Section: Acellular Biological Scaffoldsmentioning

confidence: 89%

Bioengineering of Articular Cartilage: Past, Present and Future

Felimban

Moulton

et al. 2013

Regen. Med.

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“…Recently, it has emerged as a source that is rich in both chondroinductive and chondroconductive potential. 18,20,21,[93][94][95] Current commercially available products utilizing articular cartilage and/ or DCC include Biocartilage Ò (Arthrex), De Novo NT Ò and ET Ò Live Graft (Zimmer), and Chondrofix Ò (Zimmer). However, all of these products use cartilage ECM for the purpose of regenerating only cartilage tissue.…”

Section: Figmentioning

confidence: 99%

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

Dennis

Berkland

Bonewald

et al. 2015

Tissue Engineering Part B: Reviews

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“…[4][5][6] These ECM materials can be obtained from cell-derived matrices secreted during in vitro culture or from native tissue, 4,[7][8][9][10][11] and they have either been decellularized to remove cellular components and nucleic acids or they have been devitalized to kill but not necessarily remove cells within the matrix.…”

Section: Introductionmentioning

confidence: 99%

Chondroinduction from Naturally Derived Cartilage Matrix: A Comparison Between Devitalized and Decellularized Cartilage Encapsulated in Hydrogel Pastes

Beck

Barragan

Libeer

et al. 2016

Tissue Engineering Part A

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Hydrogel precursors are liquid solutions that are prone to leaking after surgical placement. This problem was overcome by incorporating either decellularized cartilage (DCC) or devitalized cartilage (DVC) microparticles into traditional photocrosslinkable hydrogel precursors in an effort to achieve a paste-like hydrogel precursor. DCC and DVC were selected specifically for their potential to induce chondrogenesis of stem cells, given that materials that are chondroinductive on their own without growth factors are a revolutionary goal in orthopedic medicine. We hypothesized that DVC, lacking the additional chemical processing steps in DCC to remove cell content, would lead to a more chondroinductive hydrogel with rat bone marrow-derived mesenchymal stem cells. Hydrogels composed of methacrylated hyaluronic acid (MeHA) and either DCC or DVC microparticles were tested with and without exposure to transforming growth factor (TGF)-b 3 over a 6 week culture period, where swelling, mechanical analysis, and gene expression were observed. For collagen II, Sox-9, and aggrecan expression, MeHA precursors containing DVC consistently outperformed the DCC-containing groups, even when the DCC groups were exposed to TGF-b 3 . DVC consistently outperformed all TGF-b 3 -exposed groups in aggrecan and collagen II gene expression as well. In addition, when the same concentrations of MeHA with DCC or DVC microparticles were evaluated for yield stress, the yield stress with the DVC microparticles was 2.7 times greater. Furthermore, the only MeHA-containing group that exhibited shape retention was the group containing DVC microparticles. DVC appeared to be superior to DCC in both chondroinductivity and rheological performance of hydrogel precursors, and therefore DVC microparticles may hold translational potential for cartilage regeneration.

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Decellularized Cartilage Matrix as a Novel Biomatrix for Cartilage Tissue-Engineering Applications

Cited by 209 publications

References 70 publications

Bioengineering of Articular Cartilage: Past, Present and Future

Bioengineering of Articular Cartilage: Past, Present and Future

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

Chondroinduction from Naturally Derived Cartilage Matrix: A Comparison Between Devitalized and Decellularized Cartilage Encapsulated in Hydrogel Pastes

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