Investigating the scavenging of reactive oxygen species by antioxidants via theoretical and experimental methods

Jabeen, Hifza; Saleemi, Samia; Razzaq, Humaira; Yaqub, Azra; Shakoor, Saira; Qureshi, Rumana

doi:10.1016/j.jphotobiol.2018.02.006

Cited by 35 publications

(14 citation statements)

References 24 publications

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“…7C). It is well known that AA itself exhibits antioxidant capacity and radical scavenging activity 31,32 . Therefore, we hypothesized that AA suppresses cell death by attenuating ROS in a dose-dependent manner.…”

Section: Results and Disccusionmentioning

confidence: 99%

Organogermanium suppresses cell death due to oxidative stress in normal human dermal fibroblasts

Takeda

Doiyama

Azumi

et al. 2019

Sci Rep

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Reactive oxygen species (ROS) are very harmful to dermal cells, and it is thus important to develop cosmetics that protect the skin from ROS and other stimuli. Repagermanium is a synthetic water-soluble organogermanium polymer, and in this study, we attempted to visualize the incorporation of germanium into normal human dermal fibroblasts (NHDFs) using isotope microscopy. In addition, the content of 3-(trihydroxygermyl)propanoic acid (THGP), a hydrolyzed monomer of repagermanium, in NHDFs was determined through liquid chromatography mass spectrometry (LC-MS/MS), and the dose-dependent incorporation of THGP was confirmed. We then evaluated the preventive effects of THGP against ROS-induced NHDF death and confirmed the observed preventive effects through gene profiling and expression analysis. The addition of 0.59–5.9 mM THGP reduced cell death resulting from ROS damage caused by the reaction between xanthine oxidase and hypoxanthine and the direct addition of H2O2. Furthermore, this study provides the first demonstration that the effect of THGP was not due to the direct scavenging of ROS, which indicates that the mechanism of THGP differs from that of general antioxidants, such as ascorbic acid. The gene profiling and expression analysis showed that THGP suppressed the expression of the nuclear receptor subfamily 4 group A member 2 (NR4A2) gene, which is related to cell death, and the interleukin 6 (IL6) and chemokine (C-X-C motif) ligand 2 (CXCL2) genes, which are related to the inflammatory response. Furthermore, the production of IL6 induced by H2O2 was suppressed by the THGP treatment. Our data suggest that the preventive effect of THGP against ROS-induced cell death is not due to antioxidant enzymes or ROS scavenging.

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Section: Results and Disccusionmentioning

confidence: 99%

Organogermanium suppresses cell death due to oxidative stress in normal human dermal fibroblasts

Takeda

Doiyama

Azumi

et al. 2019

Sci Rep

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“…Contrastingly, an exogenous addition of vitamin E showed an alleviating effect to the senescence. It may because that vitamin E is a strong antioxidant, which can reduce the degree of oxidative damages suffered by plasma and chloroplasts during the senescence [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Vitamin E Is Superior to Vitamin C in Delaying Seedling Senescence and Improving Resistance in Arabidopsis Deficient in Macro-Elements

Zhang

Yang

Zheng

et al. 2020

IJMS

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Nitrogen (N), phosphorus (P), and potassium (K) are three essential macro-elements for plant growth and development. Used to improve yield in agricultural production, the excessive use of chemical fertilizers often leads to increased production costs and ecological environmental pollution. Vitamins C and E are antioxidants that play an important role in alleviating abiotic stress. However, there are few studies on alleviating oxidative stress caused by macro-element deficiency. Here, we used Arabidopsis vitamin E synthesis-deficient mutant vte4 and vitamin C synthesis-deficient mutant vtc1 on which exogenous vitamin E and vitamin C, respectively, were applied at the bolting stage. In the deficiency of macro-elements, the Arabidopsis chlorophyll content decreased, malondialdehyde (MDA) content and relative electric conductivity increased, and reactive oxygen species (ROS) accumulated. The mutants vtc1 and vte4 are more severely stressed than the wild-type plants. Adding exogenous vitamin E was found to better alleviate stress than adding vitamin C. Vitamin C barely affected and vitamin E significantly inhibited the synthesis of ethylene (ETH) and jasmonic acid (JA) genes, thereby reducing the accumulation of ETH and JA that alleviated the senescence caused by macro-element deficiency at the later stage of bolting in Arabidopsis. A deficiency of macro-elements also reduced the yield and germination rate of the seeds, which were more apparent in vtc1 and vte4, and adding exogenous vitamin C and vitamin E, respectively, could restore them. This study reported, for the first time, that vitamin E is better than vitamin C in delaying seedling senescence caused by macro-element deficiency in Arabidopsis.

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“…Reactive oxygen species (ROS), such as peroxyl, hydroxyl radicals, and superoxide anion, are generated as by-products by normal aerobic metabolism in cells (Jabeen et al, 2018). However, the overproduction of ROS can react readily with DNA, proteins, and lipids, leading to oxidative damage, sustained pro-inflammatory responses, and many chronic diseases (Zeng et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and antioxidant action of chitosan derivatives with amino-containing groups via azide-alkyne click reaction and N-methylation

Tan

Zhang

Zhao

et al. 2018

Carbohydrate Polymers

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Amino functionality has been paid growing attention in chemical modification of polysaccharides due to their potential biomedical applications. Here, the preparation of novel antioxidant materials based on chitosan derivatives bearing amino-containing groups equipped with 1,2,3-triazole and 1,2,3-triazolium by Cuprous-catalyzed azide-alkyne cycloaddition and N-methylation was described for the first time. The structural characteristics of the synthesized derivatives were examined by FTIR, H NMR, and elemental analysis. The antioxidant activities of the chitosan derivatives were assessed in vitro. The results indicated that chitosan derivatives bearing 1,2,3-triazoles displayed superior antioxidant activity over pristine chitosan, especially against superoxide anion radical. Moreover, antioxidant efficiency of chitosan derivatives further enhanced after N-methylation of 1,2,3-triazole moieties with iodomethane, which is comparative to that of ascorbic acid. Notably, of all chitosan derivatives bearing 1,2,3-triazole or 1,2,3-triazolium moieties, acylhydrazine-functionalized and amino-functionalized chitosan showed the stronger antioxidant capacity than hydroxyl-modified chitosan at the test concentration. Besides, the cytotoxicities of them were also evaluated in vitro on HaCaT cells. These results suggested that amino and acylhydrazine-functionalized chitosan derivatives with 1,2,3-triazolium could be used as novel antioxidant biomaterials.

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Investigating the scavenging of reactive oxygen species by antioxidants via theoretical and experimental methods

Cited by 35 publications

References 24 publications

Organogermanium suppresses cell death due to oxidative stress in normal human dermal fibroblasts

Organogermanium suppresses cell death due to oxidative stress in normal human dermal fibroblasts

Vitamin E Is Superior to Vitamin C in Delaying Seedling Senescence and Improving Resistance in Arabidopsis Deficient in Macro-Elements

Synthesis and antioxidant action of chitosan derivatives with amino-containing groups via azide-alkyne click reaction and N-methylation

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