Investigating the role of vitamin C in tomato through TILLING identification of ascorbate-deficient tomato mutants

Baldet, Piérre; Brès, Cécile; Okabe, Yoshihiro; Just, Daniel; Bournonville, Celine; Ferrand, Carine; Mori, Kentaro; Ezura, Hiroshi; Rothan, Christophe

doi:10.5511/plantbiotechnology.13.0622b

Cited by 29 publications

(11 citation statements)

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“…Additionally, a study of cold and light stress in tomato fruit unveils an interaction between temperature and light to modulate synthesis, recycling and oxidation of ASC in fruit (Massot et al, 2013). Light promoted the accumulation of ASC and GSH in tomato fruit, thus supporting the hypothesis of a stimulation in ASC synthesis by light (Gautier et al, 2009; Massot et al, 2012; Baldet et al, 2013; Smirnoff, 2018).…”

Section: The Importance Of the Redox Hub For Fruit Signallingsupporting

confidence: 75%

Get the Balance Right: ROS Homeostasis and Redox Signalling in Fruit

et al. 2019

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Plant central metabolism generates reactive oxygen species (ROS), which are key regulators that mediate signalling pathways involved in developmental processes and plant responses to environmental fluctuations. These highly reactive metabolites can lead to cellular damage when the reduction-oxidation (redox) homeostasis becomes unbalanced. Whilst decades of research have studied redox homeostasis in leaves, fundamental knowledge in fruit biology is still fragmentary. This is even more surprising when considering the natural profusion of fruit antioxidants that can process ROS and benefit human health. In this review, we explore redox biology in fruit and provide an overview of fruit antioxidants with recent examples. We further examine the central role of the redox hub in signalling during development and stress, with particular emphasis on ascorbate, also referred to as vitamin C. Progress in understanding the molecular mechanisms involved in the redox regulations that are linked to central metabolism and stress pathways will help to define novel strategies for optimising fruit nutritional quality, fruit production and storage.

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Section: The Importance Of the Redox Hub For Fruit Signallingsupporting

confidence: 75%

Get the Balance Right: ROS Homeostasis and Redox Signalling in Fruit

et al. 2019

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“…In addition, according to Gould (1992), cultivars could be considered a significant source of ascorbic acid with contents higher than 200 mgÁg -1 ; on this basis, five of the eight genotypes analyzed in this work [C H , LR H , O N , P N , and R N ] would be considered a significant source of ascorbic acid because they had values >200 mgÁg -1 . At physiological pH, vitamin C exists as monoanion form called ascorbate, which has proven roles in stress tolerance in tomato and fulfills numerous roles, mainly through its antioxidant potential (Baldet et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Balance and Regulation in Tomato Genotypes of Different Color

Vela-Hinojosa¹,

Escalona‐Buendía²,

Mendoza‐Espinoza³

et al. 2019

J. Amer. Soc. Hort. Sci.

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ADDITIONAL INDEX WORDS. anthocyanins, polyphenols, flavonoids, PSY ABSTRACT. Antioxidants, antioxidant capacity, and the expression of isoprenoid metabolism-related genes and two pigmentation-related transcription factors were studied in four native and four hybrid tomato (Solanum lycopersicum) genotypes with different-colored fruit. Red fruit genotypes were associated with greater lycopene, b-carotene, lipophilic antioxidant capacity, and greater chromoplast-specific lycopene b-cyclase (CYC-B) transcript levels. Orange fruit genotypes had greater concentrations of tocopherols and greater transcript levels of homogentisate phytyl transferase (VTE-2), 1-deoxy-D-xylulose phosphate synthase (DXS), and 4-hydroxyphenylpyruvate dioxygenase (HPPD). The yellow fruit genotype was greater in total polyphenol and hydrophilic antioxidant capacity with greater expression of geranylgeranyl reductase (GGDR), phytol kinase (VTE-5), phytoene synthase (PSY) 2, lycopene b-cyclase (LCY-B), SlNAC1, and SINAC4. Greater levels of individual antioxidants were associated with specific coloration of tomato fruit. Moreover, the negative correlations between the expression of PSY1 and VTE-5, and between lycopene and chlorophyll, suggest a balance between carotenoids, tocopherols, and chlorophylls. The results of this study support either the direct commercialization of tomatoes with different color fruit or use of their genotypes in breeding programs to increase antioxidant levels among existing cultivars.

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“…Among these were missense, truncation and splice junction mutations in three different genes involved in ascorbate biosynthesis, which resulted in reduced tolerance to high light intensity in the ascorbate-deficient mutants. These results are described in a companion paper (Baldet et al 2013).…”

Section: Reverse Genetics Approach Using Micro-tom Ems Mutant Collectmentioning

confidence: 87%

Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato

Just

Garcia

Fernández

et al. 2013

Plant Biotechnology

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Tomato is currently the model plant for fleshy fruit development and for Solanaceae species. Recent genomic approaches including transcriptome, proteome and metabolome analyses and genetic mapping have produced a wealth of candidate genes whose function needs to be assessed. The recent development in model and crop plants of TILLING (Targeting Induced Local Lesions IN Genomes), which reveals allelic series corresponding to several independent point mutations, and the current availability of deep sequencing tools further increase the interest of generating artificiallyinduced genetic diversity in tomato. We describe here the generation and use of EMS (ethyl methanesulfonate) tomato mutants in the miniature cultivar Micro-Tom and provide as example the identification of new fruit size and morphology mutants. We further propose new deep sequencing-based strategies for the discovery of mutations underlying phenotypic variations observed in mutant collections that will considerably increase the interest of exploiting Micro-Tom mutant collections for gene discovery in tomato.

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Investigating the role of vitamin C in tomato through TILLING identification of ascorbate-deficient tomato mutants

Cited by 29 publications

References 28 publications

Get the Balance Right: ROS Homeostasis and Redox Signalling in Fruit

Get the Balance Right: ROS Homeostasis and Redox Signalling in Fruit

Antioxidant Balance and Regulation in Tomato Genotypes of Different Color

Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato

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