Get the Balance Right: ROS Homeostasis and Redox Signalling in Fruit

Decros, Guillaume; Baldet, Piérre; Beauvoit, Bertrand; Stevens, Rebecca; Flandin, Amélie; Colombié, Sophie; Gibon, Yves; Pétriacq, Pierre

doi:10.3389/fpls.2019.01091

Cited by 118 publications

(69 citation statements)

References 165 publications

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“…During fruit ripening, oxidative stress was increased and might result in some changes in fruit, such as changes in skin color or fruit softening. Due to the presence of ROS, fruit antioxidants might act to balance the reduction-oxidation homeostasis [42]. One of the most known fruit antioxidants is ascorbic acid.…”

Section: Discussionmentioning

confidence: 99%

GC-MS Based Metabolite Profiling to Monitor Ripening-Specific Metabolites in Pineapple (Ananas comosus)

et al. 2020

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Pineapple is one of the most cultivated tropical, non-climacteric fruits in the world due to its high market value and production volume. Since non-climacteric fruits do not ripen after harvest, the ripening stage at the time of harvest is an important factor that determines sensory quality and shelf life. The objective of this research was to investigate metabolite changes in the pineapple ripening process by metabolite profiling approach. Pineapple (Queen variety) samples from Indonesia were subjected to GC-MS analysis. A total of 56, 47, and 54 metabolites were annotated from the crown, flesh, and peel parts, respectively. From the principal component analysis (PCA) plot, separation of samples based on ripening stages from C0–C2 (early ripening stages) and C3–C4 (late ripening stages) was observed for flesh and peel parts, whereas no clear separation was seen for the crown part. Furthermore, orthogonal projection to latent structures (OPLS) analysis suggested metabolites that were associated with the ripening stages in flesh and peel parts of pineapple. This study indicated potentially important metabolites that are correlated to the ripening of pineapple that would provide a basis for further study on pineapple ripening process.

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Section: Discussionmentioning

confidence: 99%

GC-MS Based Metabolite Profiling to Monitor Ripening-Specific Metabolites in Pineapple (Ananas comosus)

et al. 2020

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“…In cucumber, brassinosteroids were reported to induce ethylene responses and ROS, the collective activities of which resulted in stimulation of AOX and activation of downstream abiotic stress responses (Wei et al, 2015). ROS, which are produced in the mitochondria during respiration and accumulate in the apoplast as a result of abiotic stimulation of respiratory burst oxidase (NADPH oxidase) homologs, were historically thought of in terms of their toxicity to plants in high concentrations; however, their critical roles in response to perturbations in cellular redox state have become clearer in recent years (Jimenez et al, 2002;Marino et al, 2012;Vaahtera et al, 2013;El-Maarouf-Bouteau et al, 2015;Kumar et al, 2016;Noctor et al, 2018;Decros et al, 2019). During fruit maturation, ROS accumulation peaks once at the start of ripening (presumably the start of the respiratory climacteric) and again at overripening, around the time of harvest maturity (Muñoz and Munné-Bosch, 2018).…”

Section: Molecular and Metabolic Links Between Respiration And Ethylenementioning

confidence: 99%

Beyond Ethylene: New Insights Regarding the Role of Alternative Oxidase in the Respiratory Climacteric

Hewitt

Dhingra

2020

Front. Plant Sci.

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Climacteric fruits are characterized by a dramatic increase in autocatalytic ethylene production that is accompanied by a spike in respiration at the onset of ripening. The change in the mode of ethylene production from autoinhibitory to autostimulatory is known as the System 1 (S1) to System 2 (S2) transition. Existing physiological models explain the basic and overarching genetic, hormonal, and transcriptional regulatory mechanisms governing the S1 to S2 transition of climacteric fruit. However, the links between ethylene and respiration, the two main factors that characterize the respiratory climacteric, have not been examined in detail at the molecular level. Results of recent studies indicate that the alternative oxidase (AOX) respiratory pathway may play an essential role in mediating cross-talk between ethylene response, carbon metabolism, ATP production, and ROS signaling during climacteric ripening. New genomic, metabolic, and epigenetic information sheds light on the interconnectedness of ripening metabolic pathways, necessitating an expansion of the current, ethylene-centric physiological models. Understanding points at which ripening responses can be manipulated may reveal key, species-and cultivarspecific targets for regulation of ripening, enabling superior strategies for reducing postharvest wastage.

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“…There were no clues for MnP inactivation via the abstraction of its stabilizing Ca 2+ cations [80,81] by the oxalate chelant. As its catalytic cycle includes the reduction of H 2 O 2 + 2 H + to 2H 2 O [1][2][3], MnP may act in analogy to plant peroxidase in the control of this stress-induced active oxygen species [93] and reduce the acidity of the substrate. Peroxide itself is primarily seen as a signaling agent rather than a toxicant [94].…”

Section: Dynamics Of Interactionmentioning

confidence: 99%

Aspects Determining the Dominance of Fomitopsis pinicola in the Colonization of Deadwood and the Role of the Pathogenicity Factor Oxalate

Gramss

2020

Forests

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Carbon and mineral cycling in sustainable forest systems depends on a microbiome of basidiomycetes, ascomycetes, litter-degrading saprobes, ectomycorrhizal, and mycoparasitic fungi that constitute a deadwood degrading consortium. The brown rot basidiomycete Fomitopsis pinicola (Swartz: Fr.) P. Karsten (Fp), as an oxalate-producing facultative pathogen, is an early colonizer of wounded trees and fresh deadwood. It replaces basidiomycetous white rot fungi and non-basidiomycetous fungal phyla in the presence of its volatilome, but poorly in its absence. With the goal of determining its dominance over the most competitive basidiomycetes and its role in fungal successions within the forest microbiome in general, Fp was exposed to the white rot fungus Kuehneromyces mutabilis (Schaeff.: Fr.) Singer & Smith (Km) in aseptic dual culture established on fertilized 100 mm-long wood dust columns in glass tubes with the inclusion of their volatilomes. For the mycelia approaching from the opposite ends of the wood dust columns, the energy-generating systems of laccase and manganese peroxidase (MnP), the virulence factor oxalate, and the exhalation of terpenes were determined by spectrophotometry, High Pressure Liquid Chromatography (HPLC), and Gas Chromatography-Mass Spectrometry (GC-MS). Km mycelia perceived the approaching Fp over 20 mm of non-colonized wood dust, reduced the laccase activity to 25%, and raised MnP to 275%–500% by gaining energy and presumably by controlling oxalate, H2O2, and the dropping substrate pH caused by Fp. On mycelial contact, Km stopped Fp, secured its substrate sector with 4 mm of an impermeable barrier region during an eruption of antimicrobial bisabolenes, and dropped from the invasion mode of substrate colonization into the steady state mode of low metabolic and defensive activity. The approaching Fp raised the oxalate production throughout to >20 g kg−1 to inactivate laccase and caused, with pH 1.4–1.7, lethal conditions in its substrate sector whose physiological effects on Km could be reproduced with acidity conditions incited by HCl. After a mean lag phase of 11 days, Fp persisting in a state of high metabolic activity overgrew and digested the debilitated Km thallus and terminated the production of oxalate. It is concluded that the factors contributing to the competitive advantage of F. pinicola in the colonization of wounded trees and pre-infected deadwood are the drastic long-term acidification of the timber substrate, its own insensitivity to extremely low pH conditions, its efficient control of the volatile mono- and sesquiterpenes of timber and microbial origin, and the action of a undefined blend of terpenes and allelopathic substances.

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Get the Balance Right: ROS Homeostasis and Redox Signalling in Fruit

Cited by 118 publications

References 165 publications

GC-MS Based Metabolite Profiling to Monitor Ripening-Specific Metabolites in Pineapple (Ananas comosus)

GC-MS Based Metabolite Profiling to Monitor Ripening-Specific Metabolites in Pineapple (Ananas comosus)

Beyond Ethylene: New Insights Regarding the Role of Alternative Oxidase in the Respiratory Climacteric

Aspects Determining the Dominance of Fomitopsis pinicola in the Colonization of Deadwood and the Role of the Pathogenicity Factor Oxalate

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