Investigating the molecular structure of plasma in type 2 diabetes mellitus and diabetic nephropathy by synchrotron Fourier-transform infrared microspectroscopy

Nimer, Refat M.; Kamel, Gihan; Obeidat, Motaz; Dahabiyeh, Lina A.

doi:10.1016/j.saa.2021.120259

Cited by 7 publications

(3 citation statements)

References 58 publications

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“…This paper describes the use of a portable RS to detect the changes in skin biochemical composition in the presence of the CHF disease. Several research teams [39–47] have analyzed the possibility of optical methods to detect different diseases based on the study of human biochemicals mainly in urine, blood serum, plasma blood or whole blood. Among various optical methods, RS has demonstrated its prospects to track changes in chemical composition with the accuracy close to the current laboratory techniques.…”

Section: Resultsmentioning

confidence: 99%

Raman spectroscopy in chronic heart failure diagnosis based on human skin analysis

Khristoforova

Bratchenko

Skuratova

et al. 2023

Journal of Biophotonics

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This work aims at studying Raman spectroscopy in combination with chemometrics as an alternative fast noninvasive method to detect chronic heart failure (CHF) cases. Optical analysis is focused on the changes in the spectral features associated with the biochemical composition changes of skin tissues. A portable spectroscopy setup with the 785 nm excitation wavelength was used to record skin Raman features. In this in vivo study, 127 patients and 57 healthy volunteers were involved in measuring skin spectral features by Raman spectroscopy. The spectral data were analyzed with a projection on the latent structures and discriminant analysis. 202 skin spectra of patients with CHF and 90 skin spectra of healthy volunteers were classified with 0.888 ROC AUC for the 10-fold cross validated algorithm. To identify CHF cases, the performance of the proposed classifier was verified by means of a new test set that is equal to 0.917 ROC AUC.

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Section: Resultsmentioning

confidence: 99%

Raman spectroscopy in chronic heart failure diagnosis based on human skin analysis

Khristoforova

Bratchenko

Skuratova

et al. 2023

Journal of Biophotonics

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“…The collected raw spectra were processed using the multivariate statistical analysis software the Unscrambler X 10.4 (CAMO Analytics, Norway), which was used as previously described [ 48 , 49 , 50 ]. Briefly, the absorption spectra in the two identified spectral regions, lipid CH stretching (2800–3050 cm −1 ) and the protein (amide I and II)-carbonyl vibrations (1485–1855 cm −1 ), were baseline-corrected and smoothed with a 7-point window using the Savitzky–Golay smoothing algorithm (2 nd polynomial order).…”

Section: Methodsmentioning

confidence: 99%

Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants

et al. 2022

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Small colony variants (SCVs) are clinically significant and linked to persistent infections. In this study, synchrotron-radiation-based Fourier transform infrared (SR-FTIR) is used to investigate the microspectroscopic differences between the SCVs of Staphylococcus aureus (S. aureus) and diabetic foot Staphylococcus epidermidis (S. epidermidis) in two main IR spectral regions: (3050–2800 cm−1), corresponding to the distribution of lipids, and (1855–1500 cm−1), corresponding to the distribution of protein amide I and amide II and carbonyl vibrations. SR-FTIR successfully discriminated between the two staphylococcal species and between the SCV and the non-SCV strains within the two IR spectral regions. Combined S. aureus SCVs (SCVhMu) showed a higher protein content relative to the non-SCV wild type. Complemented S. aureus SCV showed distinguishable differences from the SCVhMu and the wild type, including a higher content of unsaturated fatty acids. An increase in the CH2/CH3 ratio was detected in S. epidermidis SCV samples compared to the standard control. Protein secondary structure in standard S. epidermidis and SCVs consisted mainly of an α-helix; however, a new shoulder at 1635 cm−1, assigned to β-sheets, was evident in the SCV. In conclusion, SR-FTIR is a powerful method that can discriminate between staphylococci species and to differentiate between SCVs and their corresponding natural strains.

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“…The information about the principle of the synchrotron radiation-based Fourier Transform InfraRed analytical technique, how this approach is used in geochemical and in other studies, and also the preparation ways of the samples, has been reported [35][36][37][38][39][40][41][42][43][44][45][46]. Attenuated total reflection (ATR), also called internal reflection spectroscopy (IRS) [22] is often used for analysis as easily and nondestructive technique and has been used mainly for surface analysis and analysis of bulk materials [47].…”

Section: Sampling Techniques According To the Research Papermentioning

confidence: 99%

FTIR as a Method for Qualitative Assessment of Solid Samples in Geochemical Research: A Review

Tkachenko

Niedzielski

2022

Molecules

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This study aims to collect information about soil investigation by FTIR. As we know, the FTIR technique is most often used in organic and bioorganic chemistry, while in geochemistry FTIR spectroscopy is not used very often. Therefore, there is a problem with the identification and interpretation of the IR spectra of minerals contained in sediments and soils. The reason for this is a deficiency of data about characteristic wavenumbers for minerals. Therefore, this study reviews and sums up, in one place, published articles that are connected to an investigation of minerals from 2002 to 2021 (based on the Scopus database). Additionally, the present review highlights various analytical techniques (ATR-FTIR, DRIFT, 2D-IR, and SR-FTIR) and discusses some of them for geochemical study. Additionally, the study describes helpful tools in the data pre-processing of IR spectra (normalization, baseline correction, and spectral derivatives).

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Investigating the molecular structure of plasma in type 2 diabetes mellitus and diabetic nephropathy by synchrotron Fourier-transform infrared microspectroscopy

Cited by 7 publications

References 58 publications

Raman spectroscopy in chronic heart failure diagnosis based on human skin analysis

Raman spectroscopy in chronic heart failure diagnosis based on human skin analysis

Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants

FTIR as a Method for Qualitative Assessment of Solid Samples in Geochemical Research: A Review

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