Investigating the interaction of mitoxantrone with anionic surfactants by spectrofluorimetry and its application for the feasible analysis of pharmaceutical preparation and biological fluids

Abstract

The behaviour of mitoxantrone (MTX), an anthracenedione antineoplastic agent, in different types of organized medium was explored using molecular spectrofluorometry. The original fluorescence and quantum yield of MTX were augmented by about five‐fold in the aqueous buffered solution (Britton–Robinson, pH 3.0) by the addition of sodium dodecyl sulfate. Enhancement in the fluorescence intensity did not come from the boost in the ultraviolet (UV) light absorbance of the drug in the presence of micelles but due to shielding of the lowest excited singlet state of the drug from a radiationless process inside the cavity of the micelle. Accordingly, a versatile, sensitive, and feasible spectrofluorimetric method was constructed and evaluated for MTX determination. Fluorescence measurements were performed at 675 nm (λex 610 nm). A linear relationship was shown between fluorescence intensity and drug concentration within the range 0.01–2.0 μg ml−1 of MTX with a correlation coefficient of 0.9999 and a detection limit of 2 ng ml−1. The developed method was effectively used for analysis of MTX in biological samples and dosage forms. In addition, the method was expanded to study the stability of MTX exposed to different drastic degradations and the kinetic parameters of the degradation were calculated.

A simple single jar “on–off fluorescence” designed system for the determination of mitoxantrone using an eosin Y dye in raw powder, vial, and human biofluids

“…Many dispersing solvents were investigated, including ethyl alcohol, methyl alcohol, 2-propanol, acetone, dioxane, and distilled water (ESI, 6.1 † ). For the designed spectroscopic approach, the greatest fluorescence quenching values were gained when distilled water (the best green solvent 38 ) was the dispersion medium. The low response obtained with organic solvents in the present study may be due to the destructive effect of these solvents on the formed complex.…”

“…The recommended spectroscopic methodology was employed to assess MTX in its pharmaceutical prescription form (Santrone® vial). The reported spectrofluorimetric technique 38 was also used to examine the same dosage form. Using two statistical tests ( t -test and F -test), the percentage recovery values of the reported spectrophotometric technique were statistically rated against those obtained from the developed system.…”

“…A suitable volume of this mixture was adjusted to the operating pH value with 0.2 M NaOH contained in another pot. 28,30,38 A Teorell-Stenhagen controlling solution with a wide pH range (2.0-12.0). 22 A McIlvaine buffer solution (pH 2.2-8.0) is composed of two separate solutions; 0.2 M citric acid and 0.1 M Na 2 HPO 4 .…”

“…The tubes were shaken vigorously, and the protein was extracted by adding an adequate amount of acetonitrile. 27,30,38 The tubes were spun for about two minutes before being centrifuged at 4000 rpm for about 10 minutes. Then, 1.0 mL of the translucent supernatant was quantitatively transferred to 5 mL volumetric asks and monitored as described above.…”

“…The treated ltrate aliquots were then accurately shied to a 5 mL standardized volumetric ask, and the evaluation steps were followed. 30,35,38…”