Investigating the human immunodeficiency virus type 1-infected monocyte-derived macrophage secretome

Ciborowski, Paweł; Kadiu, Irena; Rozek, Wojciech; Smith, Lynette; Bernhardt, Kristen; Fladseth, Melissa; Ricardo-Dukelow, Mary; Gendelman, Howard E.

doi:10.1016/j.virol.2007.01.013

Cited by 76 publications

(88 citation statements)

References 52 publications

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“…The identified cytoskeletal proteins, including actin and profilin 1, which met the selection criteria are shown in Table I. Other proteins identified were reported elsewhere (22). In agreement with the SELDI-TOF results, profilin 1 and actin were identified.…”

Section: Cytoskeletal Proteinssupporting

confidence: 77%

Cytoskeletal Protein Transformation in HIV-1-Infected Macrophage Giant Cells

Kadiu

Ricardo-Dukelow

Ciborowski

et al. 2007

The Journal of Immunology

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The mechanisms linking HIV-1 replication, macrophage biology, and multinucleated giant cell formation are incompletely understood. With the advent of functional proteomics, the characterization, regulation, and transformation of HIV-1-infected macrophage-secreted proteins can be ascertained. To these ends, we performed proteomic analyses of culture fluids derived from HIV-1 infected monocyte-derived macrophages. Robust reorganization, phosphorylation, and exosomal secretion of the cytoskeletal proteins profilin 1 and actin were observed in conjunction with productive viral replication and giant cell formation. Actin and profilin 1 recruitment to the macrophage plasma membrane paralleled virus-induced cytopathicity, podosome formation, and cellular fusion. Poly-l-proline, an inhibitor of profilin 1-mediated actin polymerization, inhibited cytoskeletal transformations and suppressed, in part, progeny virion production. These data support the idea that actin and profilin 1 rearrangement along with exosomal secretion affect viral replication and cytopathicity. Such events favor the virus over the host cell and provide insights into macrophage defense mechanisms used to contain viral growth and how they may be affected during progressive HIV-1 infection.

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Section: Cytoskeletal Proteinssupporting

confidence: 77%

Cytoskeletal Protein Transformation in HIV-1-Infected Macrophage Giant Cells

Kadiu

Ricardo-Dukelow

Ciborowski

et al. 2007

The Journal of Immunology

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“…A similar distribution was observed with the percentage of isotope groups (S1 ϭ 17,190; S2 ϭ 17,513) with intensity CVs of Ͻ 50% (81.1% and 90.2% for CSF procurements S1 and S2, respectively). These results are similar to that previously reported for label-free quantitative proteomics measurements (33,38,39). As a measure of peptide preparation reproducibility, equivalent amounts of an internal standard protein, BSA was added to each sample prior to endoprotease digestion.…”

Section: Knowledge-based Analysis Of Proteins Identified In Csfsupporting

confidence: 87%

Alterations in Protein Regulators of Neurodevelopment in the Cerebrospinal Fluid of Infants with Posthemorrhagic Hydrocephalus of Prematurity

Morales

Townsend²,

Malone³

et al. 2012

Molecular & Cellular Proteomics

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“…Interestingly, we recently showed that cystatin B expression is increased in HIV-1-infected MDM secretome. 48 Nevertheless, we have not identified this protein in any of 12 CSF samples we examined, making its link to clinical disease unknown at the present time.…”

Section: -D Dige With Saturated Csf Labeling From Had and Nd Hiv-infmentioning

confidence: 80%

Cerebrospinal Fluid Proteomic Profiling of HIV-1-Infected Patients with Cognitive Impairment

et al. 2007

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Advanced HIV-1 infection is commonly associated with progressive immune suppression and the development of cognitive, motor, and behavior disturbances. In its most severe form, it is diagnosed as HIV-1 associated dementia (HAD) and can progress to profound functional disability and death. Despite prodigious efforts to uncover biomarkers of HAD, none can adequately reflect disease onset or progression. Thus, we developed a proteomics platform for HAD biomarker discovery and used it to perform a pilot study on cerebrospinal fluid (CSF) from HIV-1-infected people with or without HAD. A 2-dimensional electrophoresis (2-DE) map of a HAD CSF proteome was focused on differentially expressed proteins. 2-DE difference gel electrophoresis (2-D DIGE) analysis showed >90 differences in protein spots of which 20 proteins were identified. Differential expression of 6 proteins was validated by Western blot tests and included vitamin D binding protein, clusterin, gelsolin, complement C3, procollagen C-endopeptidase enhancer 1, and cystatin C. We posit that these proteins, alone or together, are potential HAD biomarkers.

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Investigating the human immunodeficiency virus type 1-infected monocyte-derived macrophage secretome

Cited by 76 publications

References 52 publications

Cytoskeletal Protein Transformation in HIV-1-Infected Macrophage Giant Cells

Cytoskeletal Protein Transformation in HIV-1-Infected Macrophage Giant Cells

Alterations in Protein Regulators of Neurodevelopment in the Cerebrospinal Fluid of Infants with Posthemorrhagic Hydrocephalus of Prematurity

Cerebrospinal Fluid Proteomic Profiling of HIV-1-Infected Patients with Cognitive Impairment

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