Investigating the Effects of Tissue-Specific Extracellular Matrix on the Adipogenic and Osteogenic Differentiation of Human Adipose-Derived Stromal Cells Within Composite Hydrogel Scaffolds

Shridhar, Arthi; Amsden, Brian G.; Gillies, Elizabeth R.; Flynn, Lauren E.

doi:10.3389/fbioe.2019.00402

Cited by 25 publications

(22 citation statements)

References 64 publications

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“…In addition, histological staining is the fastest the most commonly used method to evaluate the efficiency of cell material removal [109][110][111] , and allows direct testing of non-nucleic components. Other alternative stains include Masson's Trichome stain and Picrosirius red stain 112 , to characterize the collagen structure that gives resistance to ECM. Alcian blue staining detects GAG and Oil Red identifies the lipid content of dECM 111 .…”

Section: Characterization Of Decmmentioning

confidence: 99%

The biomimetic extracellular matrix: a therapeutic tool for breast cancer research

Tamayo-Angorrilla

Andrés

Jiménez

et al. 2022

Translational Research

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Characterization Of Decmmentioning

confidence: 99%

The biomimetic extracellular matrix: a therapeutic tool for breast cancer research

Tamayo-Angorrilla

Andrés

Jiménez

et al. 2022

Translational Research

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“…Several studies have been conducted to investigate the influence of scaffold design parameters on the cell’s mechanical and biological responses [ 74 , 80 , 81 , 82 , 83 , 84 , 85 ]. Depending on the specific tissue regeneration, the biomaterial features and geometries of the scaffold were optimized, suggesting sophisticated micro-architectures for unit cell scaffolds [ 86 ].…”

Section: Geometriesmentioning

confidence: 99%

Advances in Tissue Engineering and Innovative Fabrication Techniques for 3-D-Structures: Translational Applications in Neurodegenerative Diseases

Rey

Barzaghini

Nardini

et al. 2020

Cells

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In the field of regenerative medicine applied to neurodegenerative diseases, one of the most important challenges is the obtainment of innovative scaffolds aimed at improving the development of new frontiers in stem-cell therapy. In recent years, additive manufacturing techniques have gained more and more relevance proving the great potential of the fabrication of precision 3-D scaffolds. In this review, recent advances in additive manufacturing techniques are presented and discussed, with an overview on stimulus-triggered approaches, such as 3-D Printing and laser-based techniques, and deposition-based approaches. Innovative 3-D bioprinting techniques, which allow the production of cell/molecule-laden scaffolds, are becoming a promising frontier in disease modelling and therapy. In this context, the specific biomaterial, stiffness, precise geometrical patterns, and structural properties are to be considered of great relevance for their subsequent translational applications. Moreover, this work reports numerous recent advances in neural diseases modelling and specifically focuses on pre-clinical and clinical translation for scaffolding technology in multiple neurodegenerative diseases.

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“…In addition to DAM, DTB can also promote osteogenic differentiation of stem cells without induction factors ( Hung et al, 2016 ; Paduano et al, 2017 ). Researchers found that DAM showed enhanced adipogenic induction of ADSCs in the adipogenic induction medium, while in the osteogenic induction medium, DTB showed enhanced osteogenic induction ( Shridhar et al, 2019 ). It is important to be noted that ADSCs exhibit differentiation consistent with the induced differentiation medium even in different decellularized matrices.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have concluded that decellularized matrix of homologous tissue origin can maintain a tissue-specific cell phenotype and induce tissue-specific differentiation. When adipose mesenchymal stem cells (ADSCs) were seeded on DAM and decellularized trabecular bone (DTB)respectively, DAM could enhance adipogenic differentiation of ADSCs, while osteogenetic differentiation of ADSCs was more pronounced in DTB ( Shridhar et al, 2019 ). A study compared the cellular response to esophageal extracellular matrix (eECM) versus small intestinal submucosa extracellular matrix (SIS-ECM) and urinary bladder matrix (UBM), and found eECM retains tissue-specific characteristics that enhance the migration of esophageal stem cells and supports the formation of 3D organoids ( Keane et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Investigating the Adipogenic Effects of Different Tissue-Derived Decellularized Matrices

Tang

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Objective: Decellularized adipose-derived matrix (DAM) can promote adipogenic differentiation and adipose tissue remodeling, but the biological impact of tissue origin on DAM remains unknown. The present study aimed to investigate the effects of tissue origins on the adipogenic capacity of the decellularized matrix by comparing the cellular and tissue responses of DAM versus acellular dermal matrix (ADM).Methods: The in vitro response of adipose-derived stem/stromal cells (ADSCs) to DAM and ADM was characterized by proliferation and differentiation. The in vivo remodeling response was evaluated in the subcutaneous injection model of immunocompromised mice, using histology, protein expression, and transcriptome analysis.Results: Both DAM and ADM exhibited excellent decellularization effects and cytocompatibility. In the absence of exogenous stimuli, DAM could induce adipogenic differentiation of ADSCs compared with ADM. In the animal model, the levels of PDGF, VEGF, and ACRP30 were higher in the DAM groups than in the ADM group, and more neovascularization and extensive adipose tissue remodeling were observed. The mRNA-seq analysis indicated that the DAM implant regulated tissue remodeling by modulating Lat1/2 expression along with Hippo Signaling pathway in the early stage.Conclusion: Tissue origin can influence the biological response of the decellularized matrix. DAM can retain favorable tissue-specific characteristics after the decellularization process and have unique adipogenic effects in vitro and vivo, which can be fully utilized for soft tissue repair and regeneration.

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Investigating the Effects of Tissue-Specific Extracellular Matrix on the Adipogenic and Osteogenic Differentiation of Human Adipose-Derived Stromal Cells Within Composite Hydrogel Scaffolds

Cited by 25 publications

References 64 publications

The biomimetic extracellular matrix: a therapeutic tool for breast cancer research

The biomimetic extracellular matrix: a therapeutic tool for breast cancer research

Advances in Tissue Engineering and Innovative Fabrication Techniques for 3-D-Structures: Translational Applications in Neurodegenerative Diseases

Investigating the Adipogenic Effects of Different Tissue-Derived Decellularized Matrices

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