Investigating the Adipogenic Effects of Different Tissue-Derived Decellularized Matrices

Tang, Weiya; Qi, Jun; Wang, Qian; Qu, Yaping; Fu, Su

doi:10.3389/fbioe.2022.872897

Cited by 7 publications

(8 citation statements)

References 62 publications

(67 reference statements)

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“…However, the term "matching" cannot only refer to the numerical proximity of the modulus. Tang et al [8] compared the elastic modulus of poorly-adipose-induced acellular dermal matrix (ADM) and well-adipose-induced DAM with natural adipose tissue. The elastic modulus of either ADM or DAM is significantly different from that of adipose tissue.…”

Section: Discussionmentioning

confidence: 99%

“…The production method of the DAM was improved based on the original enzyme‐free decellularized method. [ 8 ] Briefly, residual adipose tissue was collected from healthy female patients after liposuction and transported to the laboratory at a low temperature within 2 h. Fresh adipose tissue was stratified after standing for 15 min. After removing as much blood and swelling fluid as possible, the coarse fibers in the adipose tissue were picked out by sharp forceps.…”

Section: Methodsmentioning

confidence: 99%

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Effect of radiation sterilization on the ability to induce adipose regeneration in vivo in decellularized adipose‐derived matrix

Feng

2023

Biotechnology Journal

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BackgroundDecellularized adipose‐derived matrix (DAM), a biological scaffold that can induce adipose regeneration. The balance between its sterilization efficiency and its ability to maintain in situ adipose regeneration should be considered in terminal sterilization. The purpose of this study was to investigate the effects of radiation sterilization of cobalt‐60 (60Co)with different doses on adipogenesis induced by different forms of DAM, so as to reduce radiation dose under the premise of safe and effective sterilization and ensure adipogenesis induced by DAM in vivo.MethodsHigh dose (25 kGy) and low dose (5 kGy) radiation were used to sterilize freeze‐dried and wet DAM, respectively. The sterilization efficiency, macro and micro characteristics, mechanical and mechanical properties of DAM were compared, and then implanted into the immunocompromised mice to evaluate the adipose regeneration.ResultsUnder the two radiation doses, no microbial growth was found in the freeze‐dried and wet DAM sterility tests, and no significant changes were observed in the macro and micro structures. In terms of mechanical properties, the elastic modulus of high dose freeze‐dried DAM decreased significantly (p < 0.001). In vivo animal experiments, the freeze‐dried DAM irradiated with high dose almost completely lost its function of adipogenesis in vivo. Although the wet DAM irradiated with high dose could induce fat regeneration in the early stage, the adipocyte deformation and atrophy appeared in the later stage. The freeze‐dried and wet DAM after low dose irradiation was similar to the wet DAM without irradiation in the blank control, which could maintain excellent adipogenic and angiogenic functions in vivo.ConclusionHigh dose 60Co irradiation can completely destroy the ability of freeze‐dried DAM to induce adipose regeneration in situ, while low dose irradiation (5 kGy) can effectively sterilize the DAM without damaging in vivo induced adipose regeneration. Radiation has more damage to freeze‐dried DAM than wet DAM in adipogenesis properties.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of radiation sterilization on the ability to induce adipose regeneration in vivo in decellularized adipose‐derived matrix

Feng

2023

Biotechnology Journal

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“…The procedure of decellularizing adipose tissues was accomplished following our previous protocols ( Tang et al, 2022 ). All protocols reported in this study complied with ethical regulations for work with human subjects, and the study protocol was approved by the Plastic Surgery Hospital Ethics Committee (No.…”

Section: Methodsmentioning

confidence: 99%

Multi-functional gene ZNF281 identified as a molecular biomarker in soft tissue regeneration and pan-cancer progression

Hou

Luan²,

Fu³

2023

Front. Genet.

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Regeneration and tumorigenesis are indicated as related processes, while regeneration leads to life and the outcome of tumorigenesis is death. Here, we show the upregulation of zfp281 (zinc finger 281) in our adipose de novo regeneration model through RNA-seq analysis. Then, we validated the upregulation of zfp281 in adipose regeneration via immunofluorescence. Following that, we found that ZNF281 (the human homolog of Zfp281) was upregulated in most types of cancer and related to worse prognosis in 10 tumors. We further investigated the role of ZNF281 in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), pancreatic adenocarcinoma (PAAD), and stomach adenocarcinoma (STAD) and confirmed the high accuracy in the clinical diagnostic feature. Beyond that, based on these three types of cancers, we analyzed the ZNF281-related tumor immune infiltration and DNA methylation sites and finally built risk prediction models for future disease diagnosis. Taken together, our findings provide new insights into the dual role of ZNF281, and we found that it was a potential biomarker for regeneration and tumor prognosis.

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“…Moreover, the fabricated tissues exhibit well-aligned morphological structures. Tang et al investigated the adipogenic capacity of decellularized adipose tissue compared to dermal tissue [ 174 ]. Interestingly, they found higher adipogenic differentiation using dermal ECM compared to adipose ECM in in vitro as well as in vivo experiments.…”

Section: Influence Of Materials Properties On Adipose-derived Stem Ce...mentioning

confidence: 99%

How Mechanical and Physicochemical Material Characteristics Influence Adipose-Derived Stem Cell Fate

Nellinger

Kluger

2023

IJMS

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Adipose-derived stem cells (ASCs) are a subpopulation of mesenchymal stem cells. Compared to bone marrow-derived stem cells, they can be harvested with minimal invasiveness. ASCs can be easily expanded and were shown to be able to differentiate into several clinically relevant cell types. Therefore, this cell type represents a promising component in various tissue engineering and medical approaches (e.g., cell therapy). In vivo cells are surrounded by the extracellular matrix (ECM) that provides a wide range of tissue-specific physical and chemical cues, such as stiffness, topography, and chemical composition. Cells can sense the characteristics of their ECM and respond to them in a specific cellular behavior (e.g., proliferation or differentiation). Thus, in vitro biomaterial properties represent an important tool to control ASCs behavior. In this review, we give an overview of the current research in the mechanosensing of ASCs and current studies investigating the impact of material stiffens, topography, and chemical modification on ASC behavior. Additionally, we outline the use of natural ECM as a biomaterial and its interaction with ASCs regarding cellular behavior.

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Investigating the Adipogenic Effects of Different Tissue-Derived Decellularized Matrices

Cited by 7 publications

References 62 publications

Effect of radiation sterilization on the ability to induce adipose regeneration in vivo in decellularized adipose‐derived matrix

Effect of radiation sterilization on the ability to induce adipose regeneration in vivo in decellularized adipose‐derived matrix

Multi-functional gene ZNF281 identified as a molecular biomarker in soft tissue regeneration and pan-cancer progression

How Mechanical and Physicochemical Material Characteristics Influence Adipose-Derived Stem Cell Fate

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