Investigating the effect of surfactants on lipase interfacial behaviour in the presence of bile salts

Effect of emulsifier type against the action of bile salts at oil–water interfaces

“…For this reason we only show bulk concentrations up to 3 mM. This feature suggests a rapid penetration of the bile salt into the interface (Torcello-Gómez, Maldonado-Valderrama & de Vicente et al, 2011) and possibly a synergistic effect with the remaining phospholipids, such as the formation of BS-phospholipids complexes. It is important to note here that the situation is slightly different in each case.…”

“…Moreover, by combining experiments and theory original insights have been gleaned into the role of interfacial structuring against the action of bile salts, relevant to physiological studies. Despite the fact that this system does not reflect realistic conditions in the gastrointestinal tract, the generic information obtained in this study can be extrapolated to duodenal conditions (Torcello-Gómez, Maldonado-Valderrama & de Vicente et al, 2011;. As this knowledge improves, rational strategies can be developed to improve the biological impact of foods and pharmaceuticals.…”

The role of bile salts in digestion

“…One of the key roles of BS is to prepare the surface of the fat to improve the access of lipolytic enzymes to the lipid substrates. It has been proven that addition of BS to previously covered interfaces promotes the lipase adsorption [34]. This involves the displacement of adsorbed material from the oil droplets, which will be discussed here, and the subsequent displacement of lipolysis products to facilitate the partitioning into the mixed micellar phase which will also be discussed in Section 6.…”

Effect of Gum Arabic, Gum Ghatti and Sugar Beet Pectin as Interfacial Layer on Lipid Digestibility in Oil-in-Water Emulsions

“…The adsorption properties of three types of gum at the oilwater interfaces were monitored by recording the change in interfacial tension and dilatational modulus with time using a Drop Tensiometer (Teclis, France) [13,26]. The oil drop with an area of 14.2 mm 2 was delivered using a micro syringe (Exmire) through a U-shaped stainless steel needle (internal diameter 0.56 mm) driven by a motor plunger within a thermostatically controlled glass cuvette set to 37°C, which contained gum solutions buffered with 10 mM Tris-HCl at pH 7.0.…”