INVESTIGATING NATURAL TRANSITION METAL COORDINATION ANTHOCYANIN COMPLEX IN JAVA PLUM (Syzygium cumini) FRUIT AS FREE RADICAL SCAVENGING

Abstract

This research aimed to prove the existing natural transition of Metal Coordination Anthocyanin complex (MAC) from the freeze-dried of Java plum (Syzygium cumini) fruit and its role as free radical scavenging. The Liquid Chromatography-Mass Spectrometry (LCMS) analysis showed the presence of anthocyanin in the fruit, while transition metals of Fe, Cu, and Ni were also found as detected by X-Ray Fluorescence (XRF). The Fourier Transform Infrared Spectroscopy (FTIR) spectra revealed the interaction of organic functional groups with transition metals. These facts were supported by X-Ray Powder Diffraction (XRD) and polarizing microscope images which displayed both amorphous and crystallite microstructures related to organic and bioinorganic content in the fruit. The analysis of complex compound indicated the transition metal as coordinator atom and a cyanidin acts as a chelator among anthocyanins existing in the fruit. The paramagnetic property of MAC in the fruit as a free radical scavenger was demonstrated by the reduction of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical intensity in the Electron Spin Resonance (ESR) spectrum. These findings suggested that S.cumini fruit contains naturally existing MAC.

Bioinorganic Chemistry and Computational Study of Herbal Medicine to Treatment of Tuberculosis

“…Sukmaningsih et al [14] proved that the fruit of java plum (Syzygium cumini) contains naturally occurring bioinorganic compounds. The X-ray fluorescence (XRF) characterization results indicate that the primary metal contents in java plum were K, Ca, P, Cu, Ni, and Fe.…”

“…When receiving electrons, the anthocyanin complex does not produce new radicals as well as single compound antioxidants. This is because the metal center in the flavonoid complex has paramagnetic properties (as giving electrons) that will become diamagnetic (as get electrons) when performing its functions as free radical scavengers [14]. The mechanism activity of radical scavenging is related with delocalized electron.…”

“…The active compound in herbal medicine that functions as a radical scavenger in bioinorganic chemistry is known as low molecular weight antioxidant (LMWA) complex. This LMWA contains metal ions such as Fe, Mn, Cu, and Zn [13,14]. The complex can overcome the impairing energy transfer system of the host because they can transfer electron when electron transfer system is impaired due to anemia in the case of TB infection.…”

Scavenging activity nano complex compounds of kelor (Moringa oleifera Lamk.) leaves and seeds

“…According to Sukmaningsih et al (2018), crystal is a material that has an organized three-dimension regular pattern with a directional atomic bonding and a tight order. Sukmaningsih et al (2018) investigated the existence of crystalline in the freezedried Java plum fruit. The freeze-dried java plum fruit powder process indicating a chemical reaction did not exist.…”

“…The unpaired electrons result in paramagnetic properties which urge to transform the molecule into a stable form. Electrons in every orbital must be paired and these paired electrons must rotate on the axis of the opposite direction (Sukmaningsih et al, 2018). Free radicals can be shed with antioxidant compounds.…”