Investigating glucosyltransferase inhibitory activities of polyphenols from kapur (Dryobalanops sp.) heartwood extracts

Kuspradini, Harlinda; Mitsunaga, Tohru; Mihara, Rie; Ohashi, Haruo

doi:10.1007/s11418-007-0176-1

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…Some procedures for the spectrophotometric determination of polyphenols in plant extracts are shown in Table − . It can be seen that according to the selected study, the final values were expressed as tannic, gallic, or caffeic acids and pirogallol concentrations, so the calibration curves were obtained using different phenolic compounds to estimate the total content of polyphenols.…”

Section: Resultsmentioning

confidence: 99%

Analysis of the Polyphenols Content in Medicinal Plants Based on the Reduction of Cu(II)/Bicinchoninic Complexes

Marino¹,

Sabino²,

Armando³

et al. 2009

J. Agric. Food Chem.

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A spectrophotometric method is proposed for the determination of the polyphenols content in aqueous extracts of plants. The method is based on the reduction of Cu(II) to Cu(I) by polyphenols, in the presence of bicinchoninic acid in a buffered medium (ammonium acetate, pH 7.0) with the formation of Cu(I)/BCA complexes. A calibration curve of absorbance (at 558 nm) vs tannic acid concentration is linear (r = 0.995; n = 7) with tannic acid from 0.1 to 0.7 micromol L(-1). The limit of detection and relative standard deviation were 40 nmol L(-1) (99% confidence level) and 3.8% (0.4 micromol L(-1) tannic acid, n = 7), respectively. For the aqueous extracts of Hamamelis virginiana L., Maytenus ilicifolia Mart. ex Reissek, Hydrocotyle bonariensis Lam, Annona muricata L., Myrciaria cauliflora (Mart.) O. Berg., Caesearia sylvestris Sw., Schinus terebinthifolia (Raddi), and Stryphnodendron adstringens (Mart.) Coville, the total polyphenol contents, expressed as tannic acid, were 3.5, 1.3, 2.0, 3.1, 15.4, 3.1, 9.1, and 6.9%, respectively.

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Section: Resultsmentioning

confidence: 99%

Analysis of the Polyphenols Content in Medicinal Plants Based on the Reduction of Cu(II)/Bicinchoninic Complexes

Marino¹,

Sabino²,

Armando³

et al. 2009

J. Agric. Food Chem.

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“…3 C). According to a previous study, ellagic acid inhibits the GTase activity of S. mutans [ 56 ] and S. sobrinus [ 57 ]. This study demonstrates that water is a superior extraction solvent for Rubi Fructus to inhibit biofilm formation and GTase activity of S. mutans and that ellagic acid is the active compound responsible for the extract’s inhibitory activity.…”

Section: Resultsmentioning

confidence: 99%

Synergistic inhibitory activity of Glycyrrhizae Radix and Rubi Fructus extracts on biofilm formation of Streptococcus mutans

Ham

Kim

2023

BMC Complement Med Ther

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Background Streptococcus mutans is a bacterium that causes oral diseases. Plaque, a biofilm produced by S. mutans and other bacteria, makes it difficult to remove cariogenic oral microorganisms, including biofilm producers. Glucan synthesis by glucosyltransferase is one of the mechanisms underlying plaque formation. This study demonstrates the effectiveness of inhibiting biofilm formation by interfering with the glucosyltransferase activity of S. mutans using edible herbal medicines. Methods This study investigated the inhibitory activity of Glycyrrhizae Radix extract, Rubi Fructus extract, glycyrrhizin from Glycyrrhizae Radix, and ellagic acid from Rubi Fructus against glucosyltransferase activity of S. mutans. Enzyme kinetic analysis identified the mechanism by which glycyrrhizin and ellagic acid inhibit enzyme activity. Results The conditions for synergistically inhibiting biofilm formation by combining Glycyrrhizae Radix and Rubi Fructus extracts were identified. Biofilm formation was also synergistically inhibited by mixing their respective active constituents, glycyrrhizin and ellagic acid. Glycyrrhizin and ellagic acid inhibited glucosyltransferase via noncompetitive and uncompetitive mechanisms, respectively, indicating that they inhibit it via distinct mechanisms. Conclusions This study presents an effective oral hygiene method using the synergistic activity of two natural plant extracts to inhibit biofilm formation through different inhibitory mechanisms against glucosyltransferase of S. mutans.

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“…Methanol extract of PC could inhibit tyrosinase activity with relative activity of 106 % [28]. Therefore, samples were dissolved in 2.5 mmol DMSO as much as 333 µl.…”

Section: Tyrosinase Inhibitory Activitymentioning

confidence: 99%

A Review of Sungkai (Peronema Canescens): Traditional Usage, Phytoconstituent, and Pharmacological Activities

et al. 2022

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This article review aims to evaluate the traditional usage, phytoconstituent, and pharmacological activities of Peronema canescens (PC) published between 1994 to 2021 and suggest directions for further in-depth research of PC as a medicinal resource. A literature review used relevant keywords to collect primary and secondary scientific papers from popular media databases such as Google Scholar, Scopus, PubMed, and Science Direct. The search keywords for papers included Peronema canescens, traditional usage, phytoconstituent, pharmacological activity, in vitro, in vivo, combined, or separated. Traditional usage of PC as medicine has been identified to treat various diseases such as toothache, malaria, fever, skin disorders, itching, bruising, and hypertension, as refreshing drinks, increasing stamina, and as food ingredients. Chemical constituents of PC were seven clerodane diterpenoid compounds, namely A2, A3, B1, B2, B3, C1, and D1 peronemin. Five of them are furanyl groups. Secondary metabolites contained in PC extract were phenols, triterpenoids, flavonoids, tannins, alkaloids, steroids, and saponins. In vitro, pharmacological activities of PC showed anti-plasmodium, anti-inflammatory, antioxidant, antidiabetic, cytotoxic, and antibacterial activities, had non-toxic effects, and did not cause teratogenic effects. In vivo studies of PC showed that PC could use as an immune booster, antiparasitic, anti-hyperuricemic, anti-plasmodium, antidiabetic, and antipyretic. Many phytoconstituent and pharmacological reports indicated that PC was an essential medicinal herb resource, and some of its traditional uses, including the treatment of fever, antiparasitic, anti-hypertension, malaria, and tonic drink, have been partially confirmed through modern pharmacological studies. Diterpenoids were the main active constituents. However, these crude extracts and isolated chemicals of PC required additional research to identify the effects, optimal dosage, mechanisms of action, long-term safety, and potential side effects. In addition, clinical research was necessary to support the therapeutic potential of PC.

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Investigating glucosyltransferase inhibitory activities of polyphenols from kapur (Dryobalanops sp.) heartwood extracts

Cited by 7 publications

References 18 publications

Analysis of the Polyphenols Content in Medicinal Plants Based on the Reduction of Cu(II)/Bicinchoninic Complexes

Analysis of the Polyphenols Content in Medicinal Plants Based on the Reduction of Cu(II)/Bicinchoninic Complexes

Synergistic inhibitory activity of Glycyrrhizae Radix and Rubi Fructus extracts on biofilm formation of Streptococcus mutans

A Review of Sungkai (Peronema Canescens): Traditional Usage, Phytoconstituent, and Pharmacological Activities

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