Investigating femtosecond-laser-induced two-photon photoacoustic generation

Urban, Ben E.; Yi, Ji; Yakovlev, Vladislav V.

doi:10.1117/1.jbo.19.8.085001

Cited by 11 publications

(11 citation statements)

References 24 publications

(29 reference statements)

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“…This nonlinear relationship, analogous to other nonlinear microscopies, such as two-photon fluorescent microscopy 18 and two-photon absorption-induced PAM, 19,20 can significantly improve the axial resolution of GR-PAM. In this work, an intensity-modulated CW laser, instead of a nanosecond pulsed laser as in Ref.…”

Section: Methodsmentioning

confidence: 77%

Grueneisen relaxation photoacoustic microscopyin vivo

Shi

Hai

et al. 2016

J. Biomed. Opt

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Abstract. Grueneisen relaxation photoacoustic microscopy (GR-PAM) can achieve optically defined axial resolution, but it has been limited to ex vivo demonstrations so far. Here, we present the first in vivo image of a mouse brain acquired with GR-PAM. To induce the GR effect, an intensity-modulated continuous-wave laser was employed to heat absorbing objects. In phantom experiments, an axial resolution of 12.5 μm was achieved, which is sixfold better than the value achieved by conventional optical-resolution PAM. This axial-resolution improvement was further demonstrated by imaging a mouse brain in vivo, where significantly narrower axial profiles of blood vessels were observed. The in vivo demonstration of GR-PAM shows the potential of this modality for label-free and high-resolution anatomical and functional imaging of biological tissues.

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Section: Methodsmentioning

confidence: 77%

Grueneisen relaxation photoacoustic microscopyin vivo

Shi

Hai

et al. 2016

J. Biomed. Opt

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“…With multimodal microscopy, we can obtain a more comprehensive insight into the tissue microarchitecture in living animals, including the vasculature, neurons, and extracellular matrix. A recent study has reported that an amplified femtosecond laser could excite two-photon photoacoustic signal 34 . So in future, we may try to use an amplified femtosecond laser to replace the two independent lasers in this system.…”

Section: Discussionmentioning

confidence: 99%

Fully integrated reflection-mode photoacoustic, two-photon and second harmonic generation microscopy in vivo

Song

Zhang

et al. 2016

Sci Rep

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The ability to obtain comprehensive structural and functional information from intact biological tissue in vivo is highly desirable for many important biomedical applications, including cancer and brain studies. Here, we developed a fully integrated multimodal microscopy that can provide photoacoustic (optical absorption), two-photon (fluorescence), and second harmonic generation (SHG) information from tissue in vivo, with intrinsically co-registered images. Moreover, using a delicately designed optical-acoustic coupling configuration, a high-frequency miniature ultrasonic transducer was integrated into a water-immersion optical objective, thus allowing all three imaging modalities to provide a high lateral resolution of ~290 nm with reflection-mode imaging capability, which is essential for studying intricate anatomy, such as that of the brain. Taking advantage of the complementary and comprehensive contrasts of the system, we demonstrated high-resolution imaging of various tissues in living mice, including microvasculature (by photoacoustics), epidermis cells, cortical neurons (by two-photon fluorescence), and extracellular collagen fibers (by SHG). The intrinsic image co-registration of the three modalities conveniently provided improved visualization and understanding of the tissue microarchitecture. The reported results suggest that, by revealing complementary tissue microstructures in vivo, this multimodal microscopy can potentially facilitate a broad range of biomedical studies, such as imaging of the tumor microenvironment and neurovascular coupling.

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“…Recently, OR-PAM has succeeded in suboptical-diffraction imaging, taking advantage of various nonlinear mechanisms such as two-photon absorption, 79-81 photobleaching, 82 absorption saturation, 83 and thermal relaxation. 83,84 Nonlinear OR-PAM has provided optically determined resolutions in all dimensions including the axial or depth direction.…”

Section: Optical-resolution Patmentioning

confidence: 99%

Multiscale Functional and Molecular Photoacoustic Tomography

2015

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Photoacoustic tomography (PAT) combines rich optical absorption contrast with the high spatial resolution of ultrasound at depths in tissue. The high scalability of PAT has enabled anatomical imaging of biological structures ranging from organelles to organs. The inherent functional and molecular imaging capabilities of PAT have further allowed it to measure important physiological parameters and track critical cellular activities. Integration of PAT with other imaging technologies provides complementary capabilities and can potentially accelerate the clinical translation of PAT.

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Investigating femtosecond-laser-induced two-photon photoacoustic generation

Cited by 11 publications

References 24 publications

Grueneisen relaxation photoacoustic microscopyin vivo

Grueneisen relaxation photoacoustic microscopyin vivo

Fully integrated reflection-mode photoacoustic, two-photon and second harmonic generation microscopy in vivo

Multiscale Functional and Molecular Photoacoustic Tomography

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