Investigating arsenic toxicity in tropical soils: A cell cycle and DNA fragmentation approach

Alvarenga, Ingrid Fernanda Santana; Santos, Fabio Eduardo dos; Silveira, Graciele Lurdes; Andrade-Vieira, Larissa Fonseca; Martins, Gabriel Caixeta; Guilherme, Luiz Roberto Guimarães

doi:10.1016/j.scitotenv.2019.134272

Cited by 24 publications

(7 citation statements)

References 35 publications

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“…1 The International Agency of Research on Cancer has ranked arsenic as a class-1 carcinogen. 2 Food and water contaminated with arsenic is the major source of arsenic exposure in humans, resulting in acute poisoning and chronic illness. 3 Arsenic may disturb the balance of free radicals and lead to oxidative stress.…”

Section: ■ Introductionmentioning

confidence: 99%

Metallothionein Attenuated Arsenic-Induced Cytotoxicity: The Underlying Mechanism Reflected by Metabolomics and Lipidomics

Wang

et al. 2021

J. Agric. Food Chem.

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Arsenic ions (As3+) have been recognized as a hazard that threatens the health of humans. Metallothionein (MT) rich in cysteine may provide favorable binding sites for chelation of As3+. However, the influence of MT on As3+-induced toxicity and the underlying mechanism are poorly understood, especially at the metabolic level. Herein, the effects of MT on As3+-induced toxicity were evaluated. Cell viability analysis suggested that MT alleviated As3+-induced cytotoxicity. The metabolic response of PC12 cells to As3+ investigated by lipidomics and metabolomics indicated that the presence of As3+ disrupted phospholipids metabolism and induced cell membrane damage. Moreover, energy and amino acid metabolism were perturbed by As3+. The perturbation of As3+ on metabolism was further illustrated by the decrease of the mitochondrial membrane potential and the rise of cellular reactive oxygen species (ROS). On the contrary, MT rescued As3+-induced metabolic disorder and suppressed ROS accumulation. In addition, the binding process between As3+ and MT was characterized. The results proved that the As3+–MT complex was formed and chelated As3+-scavenged ROS, thus alleviating the toxic effects of As3+. These results revealed that MT would be a potential agent to reduce As3+-induced cytotoxicity.

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Section: ■ Introductionmentioning

confidence: 99%

Metallothionein Attenuated Arsenic-Induced Cytotoxicity: The Underlying Mechanism Reflected by Metabolomics and Lipidomics

Wang

et al. 2021

J. Agric. Food Chem.

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“…Once inside plant cells, As affects growth and development of numerous physiological, biochemical and molecular systems (Farooq et al 2016). At cellular level, As toxicity disrupts the cell cycle, causes chromosomal abnormalities and damages DNA in onion (Allium cepa) cells (Alvarenga et al 2020). Arsenic also alters plant anatomy as it reduces the cortex area, damages cells in outer layers leading to dark deposits in cortex cells, xylem vessel elements and phloem cell walls in soybean (Glycine max) (Armendariz et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Phosphorus supplementation modulates nitric oxide biosynthesis and stabilizes the defence system to improve arsenic stress tolerance in mustard

Khan

Jahan

et al. 2021

Plant Biol J

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The interaction of mineral nutrients with metals/metalloids and signalling molecules is well known. In the present study, we investigated the effect of phosphorus (P) in mitigation of arsenic (As) stress in mustard (Brassica juncea L.).• The study was conducted to investigate potential of 30 mg PÁkg À1 soil P supplement (diammonium phosphate) to cope up with the adverse effects of As stress (24 mg AsÁkg À1 soil) in mustard plants • Supplementation of P influenced nitric oxide (NO) generation, which up-regulated proline metabolism, ascorbate-glutathione system and glyoxalase system and alleviated the effects of on photosynthesis and growth. Arsenic stress generated ROS and methylglyoxal content was scavenged through P-mediated NO, and reduced As translocation from roots to leaves. The involvement of NO under P-mediated alleviation of As stress was substantiated with the use of cPTIO (NO biosynthesis inhibitor) and SNP (NO inducer). The reversal of P effects on photosynthesis under As stress with the use of cPTIO emphasized the role of P-mediated NO in mitigation of As stress and protection of photosynthesis • The results suggested that P reversed As-induced oxidative stress by modulation of NO formation, which regulated antioxidant machinery. Thus, P-induced regulatory interaction between NO and reversal of As-induced oxidative stress for the protection of photosynthesis may be suggested for sustainable crops.

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“…Nowadays, the interactions of nanoparticles with plants have become a new field in micronuclei assays [7][8][9]. Currently, the testing is mainly performed in Allium, Nicotiana, and Vicia [10][11][12][13] and other model plants [7,[14][15][16][17].…”

Section: The Importance Of the Micronucleus Assay In Plantsmentioning

confidence: 99%

Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives

Kwaśniewska

Bara

2022

IJMS

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Cytogenetic approaches play an essential role as a quick evaluation of the first genetic effects after mutagenic treatment. Although labor-intensive and time-consuming, they are essential for the analyses of cytotoxic and genotoxic effects in mutagenesis and environmental monitoring. Over the years, conventional cytogenetic analyses were a part of routine laboratory testing in plant genotoxicity. Among the methods that are used to study genotoxicity in plants, the micronucleus test particularly represents a significant force. Currently, cytogenetic techniques go beyond the simple detection of chromosome aberrations. The intensive development of molecular biology and the significantly improved microscopic visualization and evaluation methods constituted significant support to traditional cytogenetics. Over the past years, distinct approaches have allowed an understanding the mechanisms of formation, structure, and genetic activity of the micronuclei. Although there are many studies on this topic in humans and animals, knowledge in plants is significantly limited. This article provides a comprehensive overview of the current knowledge on micronuclei characteristics in plants. We pay particular attention to how the recent contemporary achievements have influenced the understanding of micronuclei in plant cells. Together with the current progress, we present the latest applications of the micronucleus test in mutagenesis and assess the state of the environment.

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Investigating arsenic toxicity in tropical soils: A cell cycle and DNA fragmentation approach

Cited by 24 publications

References 35 publications

Metallothionein Attenuated Arsenic-Induced Cytotoxicity: The Underlying Mechanism Reflected by Metabolomics and Lipidomics

Metallothionein Attenuated Arsenic-Induced Cytotoxicity: The Underlying Mechanism Reflected by Metabolomics and Lipidomics

Phosphorus supplementation modulates nitric oxide biosynthesis and stabilizes the defence system to improve arsenic stress tolerance in mustard

Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives

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