Investigating Aptamer-Functionalized Silver Nanoparticles as SERS Substrates for Selective Protein Detection

Gribanev, D. A.; Zavyalova, Elena; Gambaryan, Alexandra; Кукушкин, В. И.; Рудакова, Е. В.; Ambartsumyan, Oganes

doi:10.3103/s1062873821020106

Cited by 3 publications

(4 citation statements)

References 21 publications

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“…Influenza A virus can adsorb unmodified silver nanoparticles [ 20 ]. It was shown in [ 21 ] that modifying nanoparticles with an aptamer to a certain protein results in both specific and nonspecific sorption. As this work shows, carefully selecting the necessary conditions allows us to separate specific and nonspecific sorption on nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

Using a Biosensor Based on Surface-Enhanced Raman Scattering to Identify Influenza Viruses in Biological Fluids

Zhdanov

Gribanyov

Gambaryan

et al. 2022

Bull. Russ. Acad. Sci. Phys.

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Biosensors based on the effect of surface-enhanced Raman scattering obtained on silver nanoclusters modified with DNA aptamers allow viruses to be detected with high sensitivity. However, measurements in biological media are complicated by the nonspecific sorption of biomolecules on silver. Conditions for preparing samples of biological fluids that allow the nonspecific sorption of biomolecules to be nullified are studied.

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Section: Resultsmentioning

confidence: 99%

Using a Biosensor Based on Surface-Enhanced Raman Scattering to Identify Influenza Viruses in Biological Fluids

Zhdanov

Gribanyov

Gambaryan

et al. 2022

Bull. Russ. Acad. Sci. Phys.

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“…Recently, we have demonstrated the efficiency of several sensors based on the RHA0385 aptamer ( Kukushkin et al, 2019 ; Gribanev et al, 2021 ; Gribanyov et al, 2021 ; Zhdanov et al, 2022 ). Here, we provided data about the selectivity of the aptamer toward influenza A virus and assembling of sandwich-like structures to verify the hypothetic model of the sensor.…”

Section: Resultsmentioning

confidence: 99%

“…SERS-based techniques provide high sensitivity with a short time of analysis. We aimed to minimize the time of analysis to 15 min in this work and our previous works ( Kukushkin et al, 2019 ; Gribanev et al, 2021 ; Gribanyov et al, 2021 ; Zavyalova et al, 2022 ; Zhdanov et al, 2022 ), which places the techniques in the raw of rapid point-of-care tests. SERS equipment is of rather high cost; here, we used a portable device with the price 15,000 USD with a user-friendly interface (RaPort instrument from Enhanced Spectrometry, United States).…”

Section: Discussionmentioning

confidence: 99%

“…Our previous efforts produced a SERS-based aptasensor on the solid substrate with an LoD of 5 10 –4 HAU/ml that corresponds to 8 10 4 VP/ml ( Kukushkin et al, 2019 ) and a SERS-based aptasensor on the aggregated colloid silver nanoparticles with an LoD of 2 10 5 VP/ml ( Gribanev et al, 2021 ; Gribanyov et al, 2021 ; Zavyalova et al, 2021 ; Zhdanov et al, 2022 ); both were based on the RHA0385 aptamer to hemagglutinin of the influenza A virus ( Shiratori et al, 2014 ). Despite being less sensitive and inferior in a range of virus determination, the latter is preferred because colloid nanoparticles are much cheaper and easier in the production not requiring sophisticated equipment and silicon substrates.…”

Section: Introductionmentioning

confidence: 99%

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A Combination of Membrane Filtration and Raman-Active DNA Ligand Greatly Enhances Sensitivity of SERS-Based Aptasensors for Influenza A Virus

et al. 2022

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Biosensors combining the ultrahigh sensitivity of surface-enhanced Raman scattering (SERS) and the specificity of nucleic acid aptamers have recently drawn attention in the detection of respiratory viruses. The most sensitive SERS-based aptasensors allow determining as low as 104 virus particles per mL that is 100-fold lower than any antibody-based lateral flow tests but 10–100-times higher than a routine polymerase chain reaction with reversed transcription (RT-PCR). Sensitivity of RT-PCR has not been achieved in SERS-based aptasensors despite the usage of sophisticated SERS-active substrates. Here, we proposed a novel design of a SERS-based aptasensor with the limit of detection of just 103 particles per ml of the influenza A virus that approaches closely to RT-PCR sensitivity. The sensor utilizes silver nanoparticles with the simplest preparation instead of sophisticated SERS-active surfaces. The analytical signal is provided by a unique Raman-active dye that competes with the virus for the binding to the G-quadruplex core of the aptamer. The aptasensor functions even with aliquots of the biological fluids due to separation of the off-target molecules by pre-filtration through a polymeric membrane. The aptasensor detects influenza viruses in the range of 1·103–5·1010 virus particles per ml.

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Surface-enhanced Raman spectroscopy substrate based on silver/titanium dioxide nanocomposites and demonstration on rhodamine 6G and severe acute respiratory syndrome coronavirus 2 spike-1 protein

Bai

Niu

Yang

et al. 2022

Spectroscopy Letters

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Investigating Aptamer-Functionalized Silver Nanoparticles as SERS Substrates for Selective Protein Detection

Cited by 3 publications

References 21 publications

Using a Biosensor Based on Surface-Enhanced Raman Scattering to Identify Influenza Viruses in Biological Fluids

Using a Biosensor Based on Surface-Enhanced Raman Scattering to Identify Influenza Viruses in Biological Fluids

A Combination of Membrane Filtration and Raman-Active DNA Ligand Greatly Enhances Sensitivity of SERS-Based Aptasensors for Influenza A Virus

Surface-enhanced Raman spectroscopy substrate based on silver/titanium dioxide nanocomposites and demonstration on rhodamine 6G and severe acute respiratory syndrome coronavirus 2 spike-1 protein

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