“…Our previous efforts produced a SERS-based aptasensor on the solid substrate with an LoD of 5 10 –4 HAU/ml that corresponds to 8 10 4 VP/ml ( Kukushkin et al, 2019 ) and a SERS-based aptasensor on the aggregated colloid silver nanoparticles with an LoD of 2 10 5 VP/ml ( Gribanev et al, 2021 ; Gribanyov et al, 2021 ; Zavyalova et al, 2021 ; Zhdanov et al, 2022 ); both were based on the RHA0385 aptamer to hemagglutinin of the influenza A virus ( Shiratori et al, 2014 ). Despite being less sensitive and inferior in a range of virus determination, the latter is preferred because colloid nanoparticles are much cheaper and easier in the production not requiring sophisticated equipment and silicon substrates.…”