Abstract:The aim of research: to synthesize the gene, coding the non-toxic variant of diphtheria toxin CRM197, to explore its expression in Escherichia coli cells, to construct bacterial strain - producer of recombinant CRM197 and to develop the method for purification of this CRM197 protein from the biomass of bacteria. Materials and methods of research. the gene of CRM197 was synthesized by chemical and enzymatic methods. Expression vector pColdII-CRM197 construction and assembly were performed by standard genetic en… Show more
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