Inverting microwell array chip for the cultivation of human induced pluripotent stem cells with controlled aggregate size and geometrical arrangement

Abstract

We present a novel cell culture chip, namely, "inverting microwell array chip," for cultivation of human induced pluripotent stem cells. The chip comprises a lower hydrogel microwell array and an upper polystyrene culture surface. We demonstrate the formation of uniform cellular aggregates in the microwell array, and after inversion, a culture with controlled aggregate size and geometrical arrangement on the polystyrene surface. Here, we report effects of cell concentrations on a cultivation sequence in the chip. V C 2014 AIP Publishing LLC.

Acylhydrazone-based supramolecular assemblies undergoing a converse sol-to-gel transition on trans → cis photoisomerization

“…In situ sol-to-gel transition generated valves Microuidic technologies have been recently regarded as an advanced tool to investigate non-equilibrium assemblies and controlled reactive processes in self-assembly research. [61][62][63][64] Introducing stimuli-responsive materials as a valve would realize the non-contact microuidic ow control, [65][66][67][68] probably producing simplied and versatile microuidic devices. Nevertheless, most reported valves were based on temperature-and electro-responsive systems, 69,70 which are not easy to achieve ow control in situ and are comparatively complicated.…”