Advances in microfluidic platforms for analyzing and regulating human pluripotent stem cells

Qian, Tongcheng; Shusta, Eric V.; Palecek, Sean P.

doi:10.1016/j.gde.2015.07.007

Cited by 18 publications

(9 citation statements)

References 42 publications

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“…There are numerous sources of cell types, such as ex vivo tissue, immortalized cell lines, primary cells, embryonic stem cells, or hiPSCs ( Table 1 ). Different types of cells can be sustained viably over long periods to allow the development of normal tissue architecture or diseases and enable us to measure and monitor any pathological or physiological interactions when the cells are exposed to a drug.…”

Section: Cell Sourcesmentioning

confidence: 99%

Organ‐on‐a‐Chip Systems: Microengineering to Biomimic Living Systems

Zheng

Cheng

et al. 2016

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"Organ-on-a-chip" systems integrate microengineering, microfluidic technologies, and biomimetic principles to create key aspects of living organs faithfully, including critical microarchitecture, spatiotemporal cell-cell interactions, and extracellular microenvironments. This creative platform and its multiorgan integration recapitulating organ-level structures and functions can bring unprecedented benefits to a diversity of applications, such as developing human in vitro models for healthy or diseased organs, enabling the investigation of fundamental mechanisms in disease etiology and organogenesis, benefiting drug development in toxicity screening and target discovery, and potentially serving as replacements for animal testing. Recent advances in novel designs and examples for developing organ-on-a-chip platforms are reviewed. The potential for using this emerging technology in understanding human physiology including mechanical, chemical, and electrical signals with precise spatiotemporal controls are discussed. The current challenges and future directions that need to be pursued for these proof-of-concept studies are also be highlighted.

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Section: Cell Sourcesmentioning

confidence: 99%

Organ‐on‐a‐Chip Systems: Microengineering to Biomimic Living Systems

Zheng

Cheng

et al. 2016

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267

196

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“…8) [133]. Continued research into the methods by which stem cells differentiate into functional organ models on chips will contribute to improvements in stem cell methods and advances in OOAC technology [125,134].…”

Section: Stem Cell Engineeringmentioning

confidence: 99%

Organ-on-a-chip: recent breakthroughs and future prospects

et al. 2020

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BackgroundMicrofluidics is a science and technology that precisely manipulates and processes microscale fluids. It is commonly used to precisely control microfluidic (10 −9 to 10 −18 L) fluids using channels that range in size from tens to hundreds of microns and is known as a "lab-on-a-chip" [1][2][3][4]. The microchannel is small, but has a large surface area and high mass transfer, favoring its use in microfluidic technology applications including low regent usage, controllable volumes, fast mixing speeds, rapid responses, and precision control of physical and chemical properties [1,5,6]. Microfluidics integrate sample preparation, reactions, separation, detection, and basic operating units such as cell culture, sorting and cell lysis [7]. For these reasons, interest in OOAC has intensified [8]. OOAC combines a range of chemical, biological and material science disciplines [9] and was selected as one of the "Top Ten Emerging Technologies" in the World Economic Forum [10].OOAC is a biomimetic system that can mimic the environment of a physiological organ, with the ability to regulate key parameters including AbstractThe organ-on-a-chip (OOAC) is in the list of top 10 emerging technologies and refers to a physiological organ biomimetic system built on a microfluidic chip. Through a combination of cell biology, engineering, and biomaterial technology, the microenvironment of the chip simulates that of the organ in terms of tissue interfaces and mechanical stimulation. This reflects the structural and functional characteristics of human tissue and can predict response to an array of stimuli including drug responses and environmental effects. OOAC has broad applications in precision medicine and biological defense strategies. Here, we introduce the concepts of OOAC and review its application to the construction of physiological models, drug development, and toxicology from the perspective of different organs. We further discuss existing challenges and provide future perspectives for its application.

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“…Microfluidic devices provide dynamic mechanical and biochemical properties which not only support tissue differentiation and recapitulation of cell-cell and cell-ECM interactions, but also reproduce key aspects of native microenvironments in microengineered organ and tissue models. [38, 64, 65] These miniaturized devices usually consist of PDMS, polycarbonate, and off stoichiometry thiolene-epoxy (OSTE(+)). Their physical and chemical properties can be fine-tuned during fabrication using soft lithography or photolithography techniques.…”

Section: Microfluidic Devices To Mimic Stem Cell Microenvironmentmentioning

confidence: 99%

“…The dynamical stresses experienced by cells are responsible for washing away their autocrine and paracrine signals, thus affecting cell attachment, differentiation, and proliferation. [65, 67] These systems deliver chemical factors while removing cell-secreted signaling factors, allowing for precise regulation of cellular microenvironments, gene expression profiling, and cell fate. For instance, Villa-Diaz et al utilized a PDMS microfluidic system to culture hESCs colonies without affecting nutrient delivery, differentiation, and proliferation potential.…”

Section: Microfluidic Devices To Mimic Stem Cell Microenvironmentmentioning

confidence: 99%

In Vitro Microscale Models for Embryogenesis

Rico-Varela

Wan

2018

Adv. Biosys.

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Embryogenesis is a highly regulated developmental process requiring complex mechanical and biochemical microenvironments to give rise to a fully developed and functional embryo. Significant efforts have been taken to recapitulate specific features of embryogenesis by presenting the cells with developmentally relevant signals. The outcomes, however, are limited partly due to the complexity of this biological process. Microtechnologies such as micropatterned and microfluidic systems, along with new emerging embryonic stem cell-based models, could potentially serve as powerful tools to study embryogenesis. The aim of this article is to review major studies involving the culturing of pluripotent stem cells using different geometrical patterns, microfluidic platforms, and embryo/embryoid body-on-a-chip modalities. Indeed, new research opportunities have emerged for establishing in vitro culture for studying human embryogenesis and for high-throughput pharmacological testing platforms and disease models to prevent defects in early stages of human development.

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Advances in microfluidic platforms for analyzing and regulating human pluripotent stem cells

Cited by 18 publications

References 42 publications

Organ‐on‐a‐Chip Systems: Microengineering to Biomimic Living Systems

Organ‐on‐a‐Chip Systems: Microengineering to Biomimic Living Systems

Organ-on-a-chip: recent breakthroughs and future prospects

In Vitro Microscale Models for Embryogenesis

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