Inversion bridges and meiotic behavior in species hybrids of soybeans

Ahmad, Q. N.; Britten, E. J.; Byth, D. E.

doi:10.1093/oxfordjournals.jhered.a108860

Cited by 39 publications

(19 citation statements)

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“…9), thereby suggesting that the anaphase I bridge has not been broken when the centromeres moved to the opposite poles and thus resulted in the persistance of anaphase I bridge upto anaphase II/telophase II. Such a persistance of anaphase I bridges has also been reported in Trillium (Smith 1935), Tulipa (Upcott 1937), Clematis (Meurman and Therman 1939), Paeonia (Walters 1942), Rumex (Singh 1968), Vicia faba (Sjodin 1971) and soyabeans (Ahmad et al 1977). In some of the cells, 1-2 micronuclei were observed at telo phase I and telophase II (Figs.…”

Section: J M Bahl and B R Tyagi Cytologia 53supporting

confidence: 63%

A case of paracentric inversion in Papaver dubium L.

Bahl

Tyagi

1988

CYTOLOGIA

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Section: J M Bahl and B R Tyagi Cytologia 53supporting

confidence: 63%

A case of paracentric inversion in Papaver dubium L.

Bahl

Tyagi

1988

CYTOLOGIA

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“…On the basis of this evidence, a large number of plants were reported to have inversions, such as Aesculus (Upcott, 1936), Tulipa (Upcott, 1937), Fritillaria (Frankel, 1937), Zea mays (Morgan, 1950;Ting 1965), barley (Kreft, 1968), Agave (Brandham, 1969), soybeans (Ahmad et al, 1977), and Gasteria (Brandham, 1977). However, cytological evidence from paired chromosomes suggests that inversion is not responsible for all bridge-fragment configurations.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic studies on meiotic chromosome behaviors in sterile Oriental x Trumpet lily

Luo¹,

Tuyl²,

Arens³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. In order to determine the reasons for pollen sterility in lily hybrids, four diploid sterile Oriental x Trumpet (OT) lily cultivars ('Nymph', 'Gluhwein', 'Yelloween', and 'Shocking') were used to investigate the meiotic chromosome behaviors in pollen mother cells (PMCs), using genomic in situ hybridization and conventional cytological methods. At metaphase I, chromosome associations were quite variable, not only among different genotypes but also in different PMCs of the same genotype. In addition to bivalents, a certain amount of univalent, trivalents, and quadrivalents were observed in all of the investigated genotypes. In addition, ring octavalents and ring hexavalents were observed in 'Nymph'. Even dodecavalents were observed in 'Nymph'. These abnormal chromosome associations at metaphase I implied the occurrence of chromosome interchanges (translocation) in these intersectional hybrids. At anaphase-telophase, a large number of laggard chromosomes and different kinds of chromosome bridge configurations were observed. At the tetrad stage, micronuclei and polyads were also found in many PMCs. All of these abnormal chromosome behaviors in PMCs were responsible for the pollen sterility in lily hybrids.

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“…soja by Ahmad et al (1977), who found that extent of abnormalities was influenced by environmental conditions. The same abnormalities was reported by Ahmad et al (1984), who concluded that chromosome behavior and fertility depend on at least three factors-genotype, temperature and genotype temperature interaction.…”

Section: Discussionmentioning

confidence: 99%

Seed Yield is not Impaired by Chromosome Stickiness in Sodium Azide Treated Trigonella foenum-graecum

Srivastava

Kapoor

2008

cytologia

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Summary Partial or complete stickiness of chromosomes and disorientation of spindle were the major anomalies observed in meiocytes of Trigonella plants raised from seeds treated with different concentration of sodium azide for three durations. Other aberrations were unequal separation, non synchronous division, bridge, laggard, stray etc. The pollen fertility and yield showed an increase over control despite the high frequency of stickiness and spindle disorientation. Yield was positively correlated with stickiness, indicating a balancing/regulatory effect of stickiness at the time of gamete formation. Meiosis, an event of high evolutionary stability, plays a dual role as it halves the chromosome number in order to maintain ploidy level after fertilization and also ensures viability of gametes. It is a major event in the life cycle of any individual and a normal and harmonious completion of the cytological action is controlled by large number of genes, which act from pre-meiotic to post-meiotic stage. Mutation in these genes causes abnormalities that may or may not impair pollen fertility. Key wordsCytological studies constitute an important component in a breeding program involving the development of new original forms with the aid of experimental mutagenesis. The normal and harmonious course of meiosis ensures gamete viability, which if altered has a significant affect on the yield. The objective of this work was to study the meiotic behavior in pollen mother cells of sodium azide treated Trigonella foenum-graecum plants. The different concentrations of sodium azide caused meiotic abnormalities in high numbers, but surprisingly did not affect the pollen fertility and yield. The yield was significantly increased at some doses, contrary to the belief that aberration leads to abnormal gametes and hence low yield. Sodium azide has been reported to induce large variability for several characters viz. number of capsules per plant, number of seeds per capsule, size of the seeds and weight of 100 seeds in Capsicum annuum and mutants also exhibited high germination percentage and vigour over parental variety (Umalkar et al. 1981). The suitability of sodium azide, as a mutagen for T. foenum-graecum was investigated by assessing the type of cytological anomalies and correlating it with the seed yield per plant. Materials and methodsDuring our investigation, uniform sized seeds were treated with 0.10%, 0.15%, 0.20% and 0.25% freshly prepared sodium azide solution (pHϭ4), each for different durations (2 h, 4 h and 6 h) and the treated seeds after proper washing were sown along with control in pots. For meiotic studies, flower buds were taken from plants of each treatment. Appropriate sized flower buds were fixed in 1 : 3 iron saturated acetic acid and absolute alcohol for 24 h and there after transferred to 70% alcohol. Slides were prepared by squashing the anthers in Acetocarmine stain, gently heated to

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Inversion bridges and meiotic behavior in species hybrids of soybeans

Cited by 39 publications

References 0 publications

A case of paracentric inversion in Papaver dubium L.

A case of paracentric inversion in Papaver dubium L.

Cytogenetic studies on meiotic chromosome behaviors in sterile Oriental x Trumpet lily

Seed Yield is not Impaired by Chromosome Stickiness in Sodium Azide Treated Trigonella foenum-graecum

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