Cytogenetic studies on meiotic chromosome behaviors in sterile Oriental x Trumpet lily

Luo, Jian; Tuyl, J.M. van; Arens, Paul; Niu, Lixin

doi:10.4238/2013.december.13.1

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…DiscussionKinetochore and chromosome separation during abnormal meiosisThe present study and previous reports show that all the abnormal meiosis have interesting features although each have some its own characteristics in distant F 1 hybrids(Zhou et al 2008;Luo et al 2013), allotriploid(Cui et al 2022) and odd-allotetraploid lilies(Xiao et al 2022). 1) opposite poles at anaphase I.…”

supporting

confidence: 65%

Revealing the abnormal meiosis and the variation of the functional female gametes of aneuploid lily (Lilium) using genomic in situ hybridization (GISH)

Xiao,

Sun,

Zhou

2023

Euphytica

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Aneuploid lilies (Lilium) could be obtained from the LAA/LAAA × AA/AAAA hybridization; however, the characteristics of their meiosis and fertility has not been reported. In this study, an aneuploid lily, J1614, was extensively investigated for its microsporogenesis, fertility and functional eggs using conventional and modern cytogenetic methods. The results indicated that J1614 was an aneuploid Longi orum-Asiatic (LA) lily (2n = 48 = 7L + 39A + 2L/A) while 'Pearl Jason' was an autotetraploid Asiatic lily (2n = 48A); L-chromosomes of J1614 usually formed univalent while A-chromosomes associated not only predominantly trivalents, but also tetravalents, bivalents, and even univalent at metaphase I as well; clearly, both univalents and other associated chromosomes were separated and moved to opposite poles at anaphase I; besides, lagging chromosomes and micronuclei were observed during microsporogenesis. Hybridization showed that J1614, regardless of its male sterility, had better partial female fertility when tetraploid a lily was used as male than when a diploid as male. The nine seedlings of J1614 × AAAA were all aneuploid with variable total chromosomes ranging from 46 to 53, meaning that the functional eggs produced by J1614 contained variable chromosomes ranging from 22 to 29. Based on the present results, we concluded that once good lines are selected from aneuploid lilies, they may not only become cultivars through vegetative propagation, but also become parents to breed new aneuploids and realize introgression breeding. In addition, the mechanism of abnormal meiosis of aneuploid lilies was hypothesized.

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confidence: 65%

Revealing the abnormal meiosis and the variation of the functional female gametes of aneuploid lily (Lilium) using genomic in situ hybridization (GISH)

Xiao,

Sun,

Zhou

2023

Euphytica

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“…Scale bars, 50 µm (Panels A,B), 100 µm (Panels C,D), and 10 mm (Panels E,F). (Luo et al, 2013), were higher than in 'Yelloween' and 'Shocking', which have fewer bivalents (Luo et al, 2013).…”

Section: Discussionmentioning

confidence: 70%

Induction of viable 2n pollen in sterile Oriental × TrumpetLiliumhybrids

Luo

Arens

Niu

et al. 2016

The Journal of Horticultural Science and Biotechnology

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In order to induce viable 2n pollen from highly sterile diploid Oriental × Trumpet (OT) (Lilium), N 2 O was used to treat flower buds of four sterile diploid OT cultivars ('Nymph', 'Gluhwein', 'Yelloween', and 'Shocking') at different stages of meiosis. There was no pollen germination in the controls. However, after N 2 O treatment at 600 kPa, three of the OT hybrids ('Nymph', 'Gluhwein', and 'Yelloween') not only exhibited fertile flower percentages (4.3-16%), but also higher rates of pollen germination (18.8-72.5%). In addition, both the fertile flower percentages and rates of pollen germination were higher following 48 h N 2 O treatment at 600 kPa than the 24 h treatment. Following 72 h N 2 O treatment at 600 kPa, most flower buds or plants were damaged and any undamaged flowers showed abnormal anthers at the time of flowering. This indicated that 48 h of N 2 O treatment at 600 kPa was optimal to induce viable 2n pollen in three out of four sterile hybrid OT lily cultivars. These results also showed that prophase Imetaphase I was the optimum stage of meiosis at which to induce 2n pollen by N 2 O treatment in lily hybrids.

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“…Meiotic studies revealed irregularities such as bridge and the laggard formation and chromosome exclusion attributable to the erratic behavior of the extra pair in D. gigantea. Similarly, Luo et al (2013) reported the formation of bridges, paracentric inversion and other chromosome irregular behaviors in lily which was likely due to the presence of extra chromosome bodies. The extra pair of chromosomes in D. gigantea was similar to other pairs in structure and behavior, thus they merely increased chromosome number which is favored by the environment.…”

Section: Discussionmentioning

confidence: 87%

“…A handful of previous studies used cytogenetics to resolve taxonomic problems in higher plants (Naik, 1976;Lackey, 1980;Oyewole, 1987;Speta, 1998). More than a decade ago, Marasek et al (2005) and recently, Luo et al (2013) employed cytogenetics to distinguish some species of lily. Cytogenetic studies on the genus Drimia in Africa have been largely confined to plants of Eastern and Southern African origin (Oyewole, 1975b).…”

Section: Introductionmentioning

confidence: 99%

Karyo-Morphological analyses of Drimia gigantea and Drimia Viridula; Two of the three species in Altissima complex of the family hyacinthaceae

Omokanye

Animasaun

Mustapha

et al. 2023

Sri Lankan J. Biol.

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Drimia is a heterogeneous, poorly understood genus that requires major rearrangement. Chromosome behavior of Drimia gigantea and Drimia viridula; two species in D. altissima complex was studied for karyotypes transmission of extra chromosomes pair and pollen viability. Meristematic cells at the root tips of D. gigantea and D. viridula were studied by conventional cytogenetic and karyotype analysis. Chromosome numbers were 2n = 22 and 2n = 20 for D. gigantea and D. viridula, respectively. Extra chromosome pair in D. gigantea resulted in 2n = 22. Chromosome length was 55.45 mm for D. viridula and 55.50 mm for D. gigantea. Bridge formation, laggards, chromosome exclusion and formation of 9 (3.26%), 10 (90.93%) and 11(5.81%) chromosome bodies were observed in D. gigantea, D. viridula consistently formed 10 bivalents. Pollen viability was 90.63% in D. gigantea and 94.95% in D. viridula. Karyotypic evidence suggests that an extra chromosome pair in D. gigantea evolved from fragmentation of the second-largest chromosome, the extra chromosome is not a B-chromosome because it participated in cell division. High pollen viability and frequent occurrence of ten bivalents, indicated that D. viridula is closer to the progenitors of a common genetic system within D. altissima complex. Furthermore, the karyo-morphological differences support the separation of the two taxa as distinct species.

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Cytogenetic studies on meiotic chromosome behaviors in sterile Oriental x Trumpet lily

Cited by 8 publications

References 31 publications

Revealing the abnormal meiosis and the variation of the functional female gametes of aneuploid lily (Lilium) using genomic in situ hybridization (GISH)

Revealing the abnormal meiosis and the variation of the functional female gametes of aneuploid lily (Lilium) using genomic in situ hybridization (GISH)

Induction of viable 2n pollen in sterile Oriental × TrumpetLiliumhybrids

Karyo-Morphological analyses of <em>Drimia gigantea</em> and<em> Drimia Viridula</em>; Two of the three species in <em>Altissima</em> complex of the family hyacinthaceae

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