Inverse relationship between apoptosis and Bcl-2 expression in syncytiotrophoblast and fibrin-type fibrinoid in early gestation

Toki, Toshihiko

doi:10.1093/molehr/5.3.246

Cited by 29 publications

(9 citation statements)

References 36 publications

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“…Reduced fibrin concentration in the midsecretory phase endometrium is considered beneficial for embryo implantation (28). An association has also been reported between fibrin deposits and trophoblast apoptosis in pathologic pregnancies (29,30).…”

Section: Figurementioning

confidence: 72%

Protein profiling of human endometrial tissues in the midsecretory and proliferative phases of the menstrual cycle

Parmar

Gadkar-Sable

Savardekar

et al. 2009

Fertility and Sterility

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Section: Figurementioning

confidence: 72%

Protein profiling of human endometrial tissues in the midsecretory and proliferative phases of the menstrual cycle

Parmar

Gadkar-Sable

Savardekar

et al. 2009

Fertility and Sterility

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“…The attenuation of apoptosis in trophoblast by EGF may result from the restoration of trophoblast proliferation and subsequent fusion with syncytiotrophoblast replenishing anti-apoptotic proteins such as: Bcl-2, inhibitors of apoptosis proteins (IAPs) and Mdm2 all of which are present in the syncytiotrophoblast [46][47][48]. There are no reports of effects of EGF on isolated syncytiotrophoblast fragments; further study is therefore required to determine whether EGF up-regulates the expression of these anti-apoptotic proteins.…”

Section: Discussionmentioning

confidence: 99%

Epidermal growth factor rescues trophoblast apoptosis induced by reactive oxygen species

et al. 2007

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Pre-eclampsia and intrauterine growth restriction are associated with increased apoptosis of placental villous trophoblast. This may result from placental hypoperfusion, leading to the generation of reactive oxygen species (ROS). Apoptosis can be induced in villous trophoblast following exposure to oxidative stress. Epidermal growth factor (EGF) reduces trophoblast apoptosis resulting from exposure to hypoxia. We hypothesised that exposure to hydrogen peroxide, a potent generator of ROS, would induce apoptosis in term placental villous explants and that this could be reduced by treatment with EGF. Placental explants were taken from normal term pregnancies and exposed to increasing doses of hydrogen peroxide (0-1,000 microM) or to a combination of increasing doses of hydrogen peroxide and EGF (0-100 ng/ml) for either 6 or 48 h. Apoptosis was assessed by TUNEL, proliferation by Ki-67 immunostaining, necrosis by lactate dehydrogenase activity and trophoblast differentiation by human chorionic gonadotrophin (hCG) secretion in conditioned culture media. Immunoperoxidase staining was performed to identify phosphorylated-AKT (p-AKT) and phosphorylated-PI3 kinase (p-PI3k). Exposure to 1,000 microM hydrogen peroxide for 48 h induced apoptosis in placental explants. The increase in TUNEL positive nuclei predominantly localised to syncytiotrophoblast. The amount of apoptosis was reduced to control levels by treatment with 10 and 100 ng/ml EGF. Proliferation of cytotrophoblasts within villous explants was significantly reduced following exposure to 1,000 microM hydrogen peroxide, this was restored to control levels by simultaneous treatment with 10 or 100 ng/ml EGF. Neither exposure to hydrogen peroxide or EGF altered the amount of necrosis. There was increased immunostaining for pPI3K following treatment with EGF. This study shows that apoptosis may be induced in villous trophoblast following exposure to ROS, and demonstrates the anti-apoptotic effect of EGF in trophoblast, the maintenance of which is essential for normal pregnancy.

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“…An apoptotic process is also mediated via mitochondria-independent pathways that converge to the proteolytic activation of Caspase 3 (20). Antiapoptotic Bcl-2 plays an important role for preventing apoptosis in the syncytiotrophoblasts of placenta (21).…”

mentioning

confidence: 99%

Proteomic Analysis of the Extraembryonic Tissue from Cloned Porcine Embryos

Chae

Cho

Seo

et al. 2006

Molecular & Cellular Proteomics

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Cloned animals developed from somatic cell nuclear transfer (SCNT) embryos are useful resources for agricultural and medical applications. However, the birth rate in the cloned animals is very low, and the cloned animals that have survived show various developmental defects. In this report, we present the morphology and differentially regulated proteins in the extraembryonic tissue from SCNT embryos to understand the molecular nature of the tissue. We examined 26-day-old SCNT porcine embryos at which the sonogram can first detect pregnancy. The extraembryonic tissue from SCNT embryos was abnormally small compared with the control. In the proteomic analysis with the SCNT extraembryonic tissue, 39 proteins were identified as differentially regulated proteins. Among up-regulated proteins, Annexins and Hsp27 were found. They are closely related to the processes of apoptosis. Among down-regulated proteins, Peroxiredoxins and anaerobic glycolytic enzymes were identified. In the Western blot analysis, antioxidant enzymes and the antiapoptotic Bcl-2 protein were down-regulated, and caspases were up-regulated. In the terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) assay with the placenta from SCNT embryos, apoptotic trophoblasts were observed. These results demonstrate that a major reason for the low birth rate of cloned animals is due to abnormal apoptosis in the extraembryonic tissue during early pregnancy. Molecular & Cellular Proteomics 5:1559 -1566, 2006.

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Inverse relationship between apoptosis and Bcl-2 expression in syncytiotrophoblast and fibrin-type fibrinoid in early gestation

Cited by 29 publications

References 36 publications

Protein profiling of human endometrial tissues in the midsecretory and proliferative phases of the menstrual cycle

Protein profiling of human endometrial tissues in the midsecretory and proliferative phases of the menstrual cycle

Epidermal growth factor rescues trophoblast apoptosis induced by reactive oxygen species

Proteomic Analysis of the Extraembryonic Tissue from Cloned Porcine Embryos

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