Protein profiling of human endometrial tissues in the midsecretory and proliferative phases of the menstrual cycle

Parmar, Tanu; Gadkar-Sable, Sushama; Savardekar, Lalita; Katkam, R.R.; Dharma, Shalmali; Meherji, Pervin; Puri, Chander P; Sachdeva, Geetanjali

doi:10.1016/j.fertnstert.2008.07.1734

Cited by 61 publications

(44 citation statements)

References 39 publications

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“…This is due to posttranscriptional regulatory mechanisms (mRNA export, surveillance, silencing and turnover) and posttranslational modifications, all of which can determine protein activity, localisation, turnover and interactions with other proteins (Mann & Jensen 2003). Several studies have previously sought to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles (DeSouza et al 2005, Chen et al 2009, Domínguez et al 2009, Parmar et al 2009, Rai et al 2010. There are also a number of studies describing endometrial phasespecific transcriptomic profiles (Kao et al 2002, Ace & Okulicz 2004, Evans et al 2012, Garrido-Gómez et al 2013.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy

Al-Gubory¹,

Arianmanesh²,

Garrel³

et al. 2014

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The expression and regulation of endometrial proteins are crucial for conceptus implantation and development. However, little is known about site-specific proteome profiles of the mammalian endometrium during the peri-implantation period. We utilised a two-dimensional gel electrophoresis/mass spectrometry-based proteomics approach to compare and identify differentially expressed proteins in sheep endometrium. Caruncular and intercaruncular endometrium were collected on days 12 (C12) and 16 (C16) of the oestrous cycle and at three stages of pregnancy corresponding to conceptus pre-attachment (P12), implantation (P16) and post-implantation (P20). Abundance and localisation changes in differentially expressed proteins were determined by western blot and immunohistochemistry. In caruncular endometrium, 45 protein spots (5% of total spots) altered between day 12 of pregnancy (P12) and P16 while 85 protein spots (10% of total spots) were differentially expressed between P16 and C16. In intercaruncular endometrium, 31 protein spots (2% of total spots) were different between P12 and P16 while 44 protein spots (4% of total spots) showed differential expression between C12 and C16. The pattern of protein changes between caruncle and intercaruncle sites was markedly different. Among the protein spots with implantation-related changes in volume, 11 proteins in the caruncular endometrium and six proteins in the intercaruncular endometrium, with different functions such as protein synthesis and degradation, antioxidant defence, cell structural integrity, adhesion and signal transduction, were identified. Our findings highlight the different but important roles of the caruncular and intercaruncular proteins during early pregnancy.

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Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy

Al-Gubory¹,

Arianmanesh²,

Garrel³

et al. 2014

Reproduction

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“…Interestingly, a number of proteins thought to be critical to implantation and present in uterine fluid were not detected, suggesting very low relative abundance of these proteins. Some of the same proteins including a1-antitrypsin and transferrin were also found to be increased in endometrial tissues, uterine fluid and secretions from mid-secretory phase tissues in another study using 2DE (Parmar et al 2009).…”

Section: Endometrial Receptivity and The Diagnosis Of Infertilitymentioning

confidence: 80%

Proteomics and the search for biomarkers of female reproductive diseases

Meehan¹,

Rainczuk²,

Salamonsen³

et al. 2010

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Over the past decade, high-throughput proteomics technologies have evolved considerably and have become increasingly more commonly applied to the investigation of female reproductive diseases. Proteomic approaches facilitate the identification of new disease biomarkers by comparing the abundance of hundreds of proteins simultaneously to find those specific to a particular clinical condition. Some of the best studied areas of female reproductive biology applying proteomics include gynaecological cancers, endometriosis and endometrial infertility. This review will discuss the progress that has been made in these areas and will highlight some of the emerging technologies that promise to contribute to better understanding of the female reproductive disease.

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“…Parmar et al analyzed endometrial tissue during the mid-secretory phase and compared the derived profiles with profiles of uterine fluids from the same period and endometrial biopsies of the proliferative phase and demonstrated increased concentrations of calreticulin, the beta chain of fibrinogen, adenylate kinase isoenzyme 5 and transferrin in the proliferative phase and of annexin V, alpha1-antitrypsin, creatinekinase, and peroxidoxin 6 in the midsecretory. HSP27, transferrin and alpha1-antitrypsin precursor were abundant in both endometrial tissues and uterine fluid (115). Garrido Gomez et al applied differential in-gel electrophoresis followed by MALDI-MS to investigate the proteomic differences between the receptive and non-receptive endometrium on the 5th day after progesterone administration during an IVF cycle and recognized 24 differentially expressed proteins, among which progesterone receptor membrane component 1 and annexin A6 seem to have a significant role in endometrial receptivity (116).…”

Section: Proteomics and Endometrial Receptivitymentioning

confidence: 99%