Inverse-Micelle Synthesis of Doxorubicin-Loaded Alginate/Chitosan Nanoparticles and In Vitro Assessment of Breast Cancer Cytotoxicity

Rosch, Justin G.; Winter, Hayden; DuRoss, Allison N.; Sahay, Gaurav; Sun, Conroy

doi:10.1016/j.colcom.2018.12.002

Cited by 30 publications

(16 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The amoxicillin encapsulation efficiency in Cs-Al NDs was calculated from a supernatant by triplicate, giving an efficiency of 74:98% ± 0:23. This result indicates that these nanodisks are a suitable material for a successful loading of amoxicillin and was consistent with the results reported in the literature of other chitosan/alginate nanoparticles [36,40,43]. The presence of the STPP crosslinking agent influences the encapsulation efficiency characteristic due to the formation of chitosan/alginate-TPP complex that produces an electrostatic interaction between carboxylic and amine functional groups of chitosan and amoxicillin, increasing the loading of amoxicillin in Cs-Al NDs [44].…”

Section: Encapsulation Efficacy Andsupporting

confidence: 90%

“…Accordingly, these results from the CellTiter 96® AQueous method are more reliable and the cytotoxicity pattern observed seems to be in agreement with other reports, in which higher concentration of nanoparticles is the main cause of inducing negative effects on the cell viability [58]. Among these reports, the use of alginate/chitosan and other types of nanocarriers against different cells lines was involved, and the results showed a similar trend in which a variation in the cell viability may be attributed to the selectivity with no discrimination toward the normal and cancerous cells [1,36,40,59].…”

Section: International Journal Of Polymer Sciencesupporting

confidence: 89%

“…The deformation of N-acetylglucosamine determines the possible interaction between the amine and phosphate groups in the Cs-Al NDs, which can be evidenced by the presence of the peaks around 1592 and 1529 cm -1 , as well as the peak at 1407 cm -1 indicating an antisymmetric deformation of methyl groups due to the electrostatic interaction with STTP [37][38][39]. The presence of alginate can be evidenced by the stretching vibration of carboxylic acid at 1382, 1214, and 1027 cm -1 [36,40]. The peak at 1306, 1129, 1065, and 891 cm -1 corresponded to the tertiary amide group, C-O-C glycosidic bond, C-O stretching vibration, and C-O-C stretching vibration [36,37].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

Patiño-Ruiz

Marrugo

Reyes

et al. 2020

International Journal of Polymer Science

View full text Add to dashboard Cite

We report on the synthesis of chitosan-alginate nanodisks (Cs-Al NDs) using a simple approach consisting of the ionotropic gelation method. Sodium tripolyphosphate (STPP) was used as crosslinking agent to promote the electrostatic interaction between amine groups the chitosan and hydroxyl and carboxyl groups of alginate. Scanning electron microscopy (SEM) images provided direct evidence of the morphology of the nanodisks where agglomeration was observed due to the electrostatic interaction between the functional groups. Furthermore, dynamic light scattering (DLS) showed that the hydrodynamic size of the Cs-Al NDs was 227 nm and 152 nm in pH 1.2 and pH 7.4, respectively, which is in agreement with the information observed in the SEM images. The chemical structure is presented mainly the amine and carboxyl groups due to the presence of chitosan and alginate in the nanodisks, respectively, which allow the electrostatic interaction through N-H linkages. According to the X-ray diffraction, we found that the Cs-Al NDs exhibited the typical structure of chitosan and alginate, which lead the formation of polyelectrolyte complexes. We also evaluated the encapsulation of amoxicillin in the nanodisk, obtaining a loading efficiency of 74.98%, as well as a maximum in vitro release amount of 63.2 and 52.3% at pH 1.2 and 7.4, respectively. Finally, the cytotoxicity effect of the Cs-Al nanodisks was performed in human prostatic epithelial PWR-1E and Caucasian prostate adenocarcinoma PC-3 cell lines, in which the cell viability was above 80% indicating low inhibition and determining the Cs-Al NDs as a promising technology for controlled delivery systems.

show abstract

Section: Encapsulation Efficacy Andsupporting

confidence: 90%

Section: International Journal Of Polymer Sciencesupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

Patiño-Ruiz

Marrugo

Reyes

et al. 2020

International Journal of Polymer Science

View full text Add to dashboard Cite

show abstract

“…However, due to the proliferation and metastasis of breast cancer cells (BCCs) remaining after chemotherapy, BC patients still face a relatively high recurrence rate and metastasis rate after being treated, which is the main death cause of them. 2,3 Therefore, it is still a great clinical challenge to deal with the recurrence and metastasis of BC, and it is of great clinical significance to find alternative therapies to replace traditional chemotherapy methods in solving the recurrence and metastasis caused by chemotherapy resistance. 4 A previous study reported that the disorder of histone lysine methylation intensified the invasiveness of BCCs.…”

Section: Introductionmentioning

confidence: 99%

<p>PRMT5 Promotes Aerobic Glycolysis and Invasion of Breast Cancer Cells by Regulating the LXRα/NF-κBp65 Pathway</p>

Han¹,

Wei²,

Wu³

2020

OTT

View full text Add to dashboard Cite

Objective: To explore the effects of protein arginine methyltransferase 5 (PRMT5) on the biological function of breast cancer cells (BCCs) by regulating the liver X receptor α (LXRα)/NF-κBp65 pathway. Methods: A total of 80 patients with breast cancer (BC) admitted to our hospital were collected, and 80 breast cancer tissue specimens and 80 corresponding tumor-adjacent tissue specimens were sampled from them for analysis. The reverse transcription-polymerase chain reaction (RT-PCR) was employed to determine the expression of PRMT5 mRNA in the sampled tissues, and the Western blot to determine the expression of LXRα and NF-κBp65 proteins in the tissues and cells. The patients were followed up to analyze their 3-year survival rate. Stable and transient overexpression vectors and inhibition vectors were constructed and transfected into BCCs. The cell counting kit-8 (CCK8), transwell, and flow cytometry were adopted to analyze the proliferation, invasion, and apoptosis of transfected cells, on which the effects of PRMT5 on LXRα and NF-κBp65 proteins were analyzed. Results: PRMT5 was highly expressed in BC patients, and LXRα was lowly expressed in them, which had a high diagnostic value. Patients with high expression of PRMT5 showed a poor prognosis, and the expression of PRMT5 was related to the tumor size, pathological stage, differentiation, and metastatic in BC patients. Overexpressed PRMT5 enhanced the cell proliferation, invasion, and glycolysis abilities, weakened apoptosis ability, further lowered expression of LXRα and increased expression of NF-κBp65, while inhibited PRMT5 caused opposite results in those aspects. Up-regulating the expression of LXRα suppressed the proliferation, invasion, and aerobic glycolysis of BCCs and promoted their apoptosis, while inhibiting it posed opposite effects. The rescue experiment revealed that down-regulating the expression of PRMT5 could counteract the promotion of downregulation of LXRα on proliferation, invasion and glycolysis of BCCs, and the nude mouse tumorigenesis test revealed that PRMT5 induced tumor on nude mice by mediating LXRα/NF-κBp65. Conclusion: Inhibition of the PRMT5 expression can accelerate apoptosis of BCCs and weaken their proliferation, invasion, and aerobic glycolysis through the LXRα/NF-κBp65 pathway.

show abstract

“…In vivo studies demonstrated that the formed complex nanoparticles (diameters below 200 nm) had superior sarcoma tumor inhibition rates at 83.17% and faint toxicity, compared with uncoated DOX nanoparticles and free DOX. Employing a water-in-oil emulsification technique, Rosch et al prepared DOX-loaded alginate/chitosan nanoparticles (~80 nm) [ 92 ]. It was shown that the nanoparticles were rapidly taken up by 4T1 murine breast cancer cells and produced high enough concentration to induce a therapeutic effect in vitro .…”

Section: Functionalization Strategies Of Alginate-based Platforms mentioning

confidence: 99%

Alginate-Based Platforms for Cancer-Targeted Drug Delivery

Shang

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

As an acidic, ocean colloid polysaccharide, alginate is both a biopolymer and a polyelectrolyte that is considered to be biocompatible, nontoxic, nonimmunogenic, and biodegradable. A significant number of studies have confirmed the potential use of alginate-based platforms as effective vehicles for drug delivery for cancer-targeted treatment. In this review, the focus is on the formation of alginate-based cancer-targeted delivery systems. Specifically, some general chemical and physical properties of alginate and different types of alginate-based delivery systems are discussed, and various kinds of alginate-based carriers are introduced. Finally, recent innovative strategies to functionalize alginate-based vehicles for cancer targeting are described to highlight research towards the optimization of alginate.

show abstract

Inverse-Micelle Synthesis of Doxorubicin-Loaded Alginate/Chitosan Nanoparticles and In Vitro Assessment of Breast Cancer Cytotoxicity

Cited by 30 publications

References 28 publications

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

<p>PRMT5 Promotes Aerobic Glycolysis and Invasion of Breast Cancer Cells by Regulating the LXRα/NF-κBp65 Pathway</p>

Alginate-Based Platforms for Cancer-Targeted Drug Delivery

Contact Info

Product

Resources

About