InvasiveMycoleptodiscusfungal cellulitis and myositis

Abstract

We report progressive necrotizing fungal cellulitis and myositis in the leg of a patient with glioblastoma multiforme treated with temozolomide and corticosteroids. While the morphologic appearance of the isolate and its ability to grow at temperatures greater than 32°C were suggestive of Mycoleptodiscus indicus, some of the conidia were atypical for this species in that they had single septa and occasional lateral appendages. Furthermore, the isolate was different from M. indicus based on the sequencing analysis of two rDNA regions. This is the first case of Mycoleptodiscus invasive fungal disease in which the causative agent could not be resolved at the species level because of inconsistencies between morphological and molecular data.

Inhibition of quorum sensing–associated virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1 by Mycoleptodiscus indicus PUTY1

“…Mycoleptodiscus indicus, a dematiaceous hyphomycete, has occasionally been reported as pathogenic because it rarely infects human beings. Until now, only 3 cases have been reported and all probably initiated by direct transmission of M. indicus from the floral part[47]. M. indicus PUTY1 has been isolated for the first time from Tridax procumbens.…”

Re-evaluation of Mycoleptodiscus species and morphologically similar fungi

“…Habitat — On leaves of monocotyledons plants such as Alloteropsis semialata , Chlorophytum capense , and Dianella congesta (Sutton & Alcorn 1990, Alcorn 1994), endophyte from Banksia verticillata (Andrioli et al 2012, as M. indicus ) and Opuntia ficus-indica (Bezerra et al 2012), fresh water foam (Ramesh & Vijaykumar 2005), Homo sapiens (Padhye et al 1995, Garrison et al 2008, Dewar & Sigler 2010, Koo et al 2012, as M. indicus ), Canis lupus subsp. familiaris (Metry et al 2010, as M. indicus ), and Felis catus (Hull et al 1997).…”

“…Additionally, some of those strains showed conidia with lateral appendages (Dewar & Sigler 2010, Metry et al 2010). Besides M. indicus , only one case of phaeohyphomycosis was reported as caused by M. lateralis in a cat (Hull et al 1997), while Koo et al (2012) were unable to identify at the species level one strain isolated from a man with glioblastoma multiforme, due to inconsistencies between the morphological and molecular data.…”

“…Furthermore, analyses of sequences deposited in the GenBank database as M. indicus or Mycoleptodiscus sp. (Dewar & Sigler 2010, Metry et al 2010, Koo et al 2012) showed that they are related to Muyocopron ( Muyocopronales ). Muyocopron was proposed by Spegazzini (1881), re-described by Saccardo (1883), and its taxonomical placement treated initially in Microthyriaceae (Von Arx & Müller 1975, Lumbsch & Huhndorf 2007) and later in Muyocopronaceae (Hyde et al 2013, Pang et al 2013, Mapook et al 2016a).…”

“…The infection caused by Mycoleptodiscus was also reported from a mycotic arthritis of the knee in a healthy Canadian male (Dewar & Sigler 2010). In New Zealand, Koo et al (2012) reported an association with Mycoleptodiscus from progressive necrotizing fungal cellulitis and myositis in the leg of a patient with glioblastoma multiforme. Domesticated animals were also reported with infections associated with Mycoleptodiscus species, such as a subcutaneous infection in a cat in Australia (Hull et al 1997), and in an immunosuppressed dog in the USA (Metry et al 2010).…”