Invasion process of induced deep nodular endometriosis in an experimental baboon model: similarities with collective cell migration?

Donnez, Olivier; Orellana, Renán; Kerk, Olivier Van; Dehoux, Jean‐Paul; Donnez, Jacques; Dolmans, Marie-Madeleine

doi:10.1016/j.fertnstert.2015.05.011

Cited by 44 publications

(44 citation statements)

References 33 publications

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“…Membrane localization of E-cadherin as well as ZO-1 was maintained in clusters of TbRII KO tumors24, whereas neither was maintained in TbRIIfl/fl tumors at the tumor-stromal interface24. A previous experimental study in a baboon model of endometriosis suggested that collective migration may be involved in pathophysiological processes of DIE28. In addition, cancer cells with high levels of TGF-β1 signaling failed to promote lung metastasis, caused by failure of cells to proliferate in the lungs23.…”

Section: Discussionmentioning

confidence: 97%

Effects of matrix stiffness on epithelial to mesenchymal transition-like processes of endometrial epithelial cells: Implications for the pathogenesis of endometriosis

Matsuzaki

Darcha

Pouly

et al. 2017

Sci Rep

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Endometriosis is defined as the presence of endometrial glands and stroma within extrauterine sites. Our previous study revealed an epithelial to mesenchymal transition (EMT)-like process in red peritoneal endometriosis, whereas membrane localization of E-cadherin was well maintained in epithelial cells of deep infiltrating endometriosis (DIE). Here we show that endometrial epithelial cells (EEE) grown on polyacrylamide gel substrates (PGS) of 2 kilopascal (kPa), a soft matrix, initiate a partial EMT-like process with transforming growth factor-β1 (TGF-β1) stimulation. Increasing matrix stiffness with TGF-β1 stimulation reduced the number of cell-cell contacts. Cells that retained cell-cell contacts showed decreased expression of E-cadherin and zonula occludens 1 (ZO-1) to cell-cell junctions. Few deep endometriotic epithelial cells (DEE) grown on 30-kPa PGS, which may mimic in vivo tissue compliance of DIE, retained localization of E-cadherin to cell-cell junctions with TGF-β1 treatment. Immunohistochemical analysis showed no phosphorylated Smad 2/3 nuclear localization in E-cadherin+ epithelial cells of DIE. We hypothesize that EEE may undergo an EMT-like process after attachment of endometrium to peritoneum in a TGF-β1–rich microenvironment. However, TGF-β1 signaling may be absent in DIE, resulting in a more epithelial cell-like phenotype in a rigid microenvironment.

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Section: Discussionmentioning

confidence: 97%

Effects of matrix stiffness on epithelial to mesenchymal transition-like processes of endometrial epithelial cells: Implications for the pathogenesis of endometriosis

Matsuzaki

Darcha

Pouly

et al. 2017

Sci Rep

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“…Mechanisms involved in gametogenesis, hormone patterns during estrous cycles, fertilization, embryo implantation, and maintenance of early pregnancy are common to these species, but not other mammals. Like humans, some non-human primates also menstruate (menstrual cycles of 28 days in macaques and 33 days in baboons) and undergo menopause [1,2], hence have proved vital to studies on infertility, contraception, pregnancy [2], and endometriosis [3,4] that can be extrapolated to humans.…”

Section: Introductionmentioning

confidence: 99%

“…More recently, baboons have also been used as a model to study follicle recruitment and development in vitro [5,6], ovarian tissue cryopreservation [7,8] and transplantation [9], and endometriosis [3,4], since they are similar to humans in terms of ovarian function, histology, and anatomy [9]. However, there are only a few existing studies on folliculogenesis in these animals, notably on follicle CA Amorim and CF Moya are joint first authors.…”

Section: Introductionmentioning

confidence: 99%

Morphometric characteristics of preantral and antral follicles and expression of factors involved in folliculogenesis in ovaries of adult baboons (Papio anubis)

Amorim

Moya

Donnez³

et al. 2016

J Assist Reprod Genet

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“…In a baboon model, induced deep endometriotic nodules were larger and more invasive when grafting specimens containing the junctional zone (19). Along the invasion front, increased mitotic activity, fewer adhesion molecules (20), and higher nerve fiber density were observed (21). The progressively increasing density with time suggests a potential role of nerve fibers in the development of deep endometriotic lesions (22).…”

Section: Histologic and Biological Basis Of Medical Therapy For Deep mentioning

confidence: 99%

Role of medical therapy in the management of deep rectovaginal endometriosis

Vercellini

Buggio

Somigliana

2017

Fertility and Sterility

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Defining whether medical therapy is effective in women with deep rectovaginal endometriosis and in which circumstances it can be considered an alternative to surgery is important for patients and physicians. Numerous observational and some randomized controlled studies demonstrated that different hormonal drugs improved pain and other symptoms in approximately two-thirds of women with deep rectovaginal endometriosis. Because major differences in the effect size of various compounds were not observed, much importance should be given to safety, tolerability, and cost of medications when counseling patients. Progestins seem to offer the best therapeutic balance when long-term treatments are planned. Women should be informed that hormonal drugs control but do not cure endometriosis and that, to avoid surgery, they should be used for years. Medical therapy is not an alternative to surgery in women with hydronephrosis, severe subocclusive bowel symptoms, and in those wishing a natural conception. A progestin should systematically be chosen as a comparator in future randomized trials on novel medications for deep endometriosis. In the meantime, the use of existing drugs should be optimized, and medical and surgical treatments could be viewed as subsequent stages of a stepwise approach. In general, there is no absolute "best" choice, and women must be thoroughly informed of potential benefits, potential harms, and costs of different therapeutic options and allowed to choose what they deem is better for them.

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Invasion process of induced deep nodular endometriosis in an experimental baboon model: similarities with collective cell migration?

Cited by 44 publications

References 33 publications

Effects of matrix stiffness on epithelial to mesenchymal transition-like processes of endometrial epithelial cells: Implications for the pathogenesis of endometriosis

Effects of matrix stiffness on epithelial to mesenchymal transition-like processes of endometrial epithelial cells: Implications for the pathogenesis of endometriosis

Morphometric characteristics of preantral and antral follicles and expression of factors involved in folliculogenesis in ovaries of adult baboons (Papio anubis)

Role of medical therapy in the management of deep rectovaginal endometriosis

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