Morphometric characteristics of preantral and antral follicles and expression of factors involved in folliculogenesis in ovaries of adult baboons (Papio anubis)

Amorim, Christiani Andrade; Moya, Cristina Fortuno; Donnez, Jacques; Dolmans, Marie-Madeleine

doi:10.1007/s10815-016-0681-9

Cited by 4 publications

(9 citation statements)

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“…For primary follicles, the number of granulosa cells that surrounded the oocyte was very variable (10–30 cells), being again similar to observation in human (Gougeon & Chainy, 1987), S. sciureus (Almeida et al , 2012), Alouatta caraya (Lopes et al , 2006), and Papio anubis (Amorim et al , 2016). As expected, the number of granulosa cells that surrounded oocytes in secondary follicles was variable (28–67 cells), similar to Alouatta caraya (Lopes et al , 2006), but much lower than found in secondary follicles from S. sciureus (Almeida et al , 2012) and Papio anubis (Amorim et al , 2016). Considering that secondary follicles are surrounded by two or more layers of granulosa cells, it is very probable that, in the present study, we measured the secondary follicles with less layers than those measured by Almeida et al (2012) & Amorim et al (2016).…”

Section: Discussionsupporting

confidence: 81%

“…As expected, the number of granulosa cells that surrounded oocytes in secondary follicles was variable (28–67 cells), similar to Alouatta caraya (Lopes et al , 2006), but much lower than found in secondary follicles from S. sciureus (Almeida et al , 2012) and Papio anubis (Amorim et al , 2016). Considering that secondary follicles are surrounded by two or more layers of granulosa cells, it is very probable that, in the present study, we measured the secondary follicles with less layers than those measured by Almeida et al (2012) & Amorim et al (2016). Therefore, it is more difficult to compare the data within this follicle class.…”

Section: Discussionsupporting

confidence: 68%

“…Dimensions of primordial, transition and primary follicles were also similar to those of Saimiri cassiquiarensis, S. macrodon and S. vanzolinii (Lopes et al, 2017), except for the oocyte nucleus, which was larger in S. apella. Moreover, preantral follicles from P. anubis (Amorim et al, 2016) were twice as big as those from S. apella.…”

Section: Discussionmentioning

confidence: 90%

“…Moreover, preantral follicles from P . anubis (Amorim et al , 2016) were twice as big as those from S . apella .…”

Section: Discussionmentioning

confidence: 95%

“…The number of granulosa cells surrounding the primordial follicles (6–11) did not differ from that observed in human (Gougeon & Chainy, 1987), S . sciureus (Almeida et al , 2012) and Alouatta caraya (Lopes et al , 2006), but it was much lower than the number of granulosa cells that surrounded the primordial follicles from Papio anubis , c. 25 cells (Amorim et al , 2016). No other authors evaluated the morphometry of transition follicles from primates.…”

Section: Discussionmentioning

confidence: 99%

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Morphology and morphometry of preantral follicles, and immunolocalization of angiogenic factors in ovarian tissue from the neotropical primateSapajus apella

Brito

Silva³

et al. 2018

Zygote

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SummaryOvarian biopsies from five health adult monkeys were collected by exploratory laparotomy. Preantral follicles (primordial, primary, and secondary) were classified as normal or degenerated and submitted to morphometric analysis in which granulosa cell counts and the areas of follicles, oocytes, and oocyte nuclei were measured. Ovarian fragments were also immunolabelled for the quantitative analysis of VEGFA and CD31 protein expression in the ovarian tissue and in the preantral follicles. In total, 213 preantral follicles was examined for morphometry and morphological classification. From this total, 20 (9.4%) were follicles enclosing two or more oocytes, i.e. multi-oocyte follicles (MOFs). From the 193 follicles enclosing only one oocyte, 46.3% were classified as primordial, 24,1% as transition, 23.3% as primary, and 6.3% as secondary follicles. The mean number of granulosa cells surrounding primordial, transition, primary, and secondary follicles was 9.2, 12.1, 18.7, and 45.3, respectively. Increase in oocyte diameter was observed from primary to secondary follicles, while the oocyte nucleus increased only when follicles reached the secondary stage. The expression of CD31 was strong in vessels, corpus luteum, and in normal oocytes and granulosa cells from preantral follicles at all developmental stages. Likewise, VEGFA expression was observed in vessels and preantral follicles (granulosa cells, the oocyte and the oocyte nucleus). We characterized the morphology, and morphometry and expression of angiogenic factors in normal and atretic preantral follicles fromSapajus apella. This description can support the analysis of follicular quality and survival after procedures such as transplantation and cryopreservation.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 90%

“…Moreover, preantral follicles from P . anubis (Amorim et al , 2016) were twice as big as those from S . apella .…”

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Morphology and morphometry of preantral follicles, and immunolocalization of angiogenic factors in ovarian tissue from the neotropical primateSapajus apella

Brito

Silva³

et al. 2018

Zygote

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Heterotopic ovarian allotransplantation in goats: Preantral follicle viability and tissue remodeling

Pinto

Alves

et al. 2020

Animal Reproduction Science

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Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

Souza

Alves

et al. 2020

PLoS ONE

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Heterotopic and orthotopic ovarian tissue autotransplantation techniques, currently used in humans, will become promising alternative methods for fertility preservation in domestic and wild animals. Thus, this study describes for the first time the efficiency of a heterotopic ovarian tissue autotransplantation technique in a large livestock species (i.e., horses) after ovarian fragments were exposed or not to a cooling process (4°C/24 h) and/or VEGF before grafting. Ovarian fragments were collected in vivo via an ultrasound-guided biopsy pick-up method and surgically autografted in a subcutaneous site in both sides of the neck in each mare. The blood flow perfusion at the transplantation site was monitored at days 2, 4, 6, and 7 post-grafting using color-Doppler ultrasonography. Ovarian grafts were recovered 7 days post-transplantation and subjected to histological analyses. The exposure of the ovarian fragments to VEGF before grafting was not beneficial to the quality of the tissue; however, the cooling process of the fragments reduced the acute hyperemia post-grafting. Cooled grafts compared with non-cooled grafts contained similar values for normal and developing preantral follicles, vessel density, and stromal cell apoptosis; lower collagen type III fibers and follicular density; and higher stromal cell density, AgNOR, and collagen type I fibers. In conclusion, VEGF exposure before autotransplantation did not improve the quality of grafted tissues. However, cooling ovarian tissue for at least 24 h before grafting can be beneficial because satisfactory rates of follicle survival and development, stromal cell survival and proliferation, as well as vessel density, were obtained.

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Morphometric characteristics of preantral and antral follicles and expression of factors involved in folliculogenesis in ovaries of adult baboons (Papio anubis)

Abstract: On the basis of these results, we can conclude that folliculogenesis in baboons appears to be similar to that in humans, and this animal therefore constitutes a valuable model.

Cited by 4 publications

References 47 publications

Morphology and morphometry of preantral follicles, and immunolocalization of angiogenic factors in ovarian tissue from the neotropical primateSapajus apella

Morphology and morphometry of preantral follicles, and immunolocalization of angiogenic factors in ovarian tissue from the neotropical primateSapajus apella

Heterotopic ovarian allotransplantation in goats: Preantral follicle viability and tissue remodeling

Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

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