Invariance and sex‐specific variations of the glomerular organization in the antennal lobes of a moth, <i>Mamestra brassicae</i>, and a butterfly, <i>Pieris brassicae</i>

Rospars, Jean-Pierre

doi:10.1002/cne.902200108

Cited by 114 publications

(122 citation statements)

References 39 publications

Supporting

Mentioning

109

Contrasting

Order By: Relevance

“…Within the scope of this study, we have found significant glomerular size increases in two of three sex-specific glomeruli in adult M. sexta. We have also found evidence for a higher variability in malespecific glomerular shape between individuals than has previously been reported (Rospars andHildebrand 1992, 2000). Some of this study has been published before in abstract form (Huetteroth and Schachtner 2003).…”

Section: Introductioncontrasting

confidence: 38%

“…The horseshoe featured the highest range of shape and volume plastici- ty: in 20 of the 37 preparations, the horseshoe comprised two halves; in six cases, we even found that three to four parts had different sizes at the corresponding position of the horseshoe (Table 1). This high variation could explain why the horseshoe neuropil was initially assigned to three different glomeruli (Rospars andHildebrand 1992, 2000). Normally, its form exhibits the shape and orientation of the toroid, but we found variations ranging from "U"-shapes to kidney shapes.…”

Section: Organization Of Al Neuropilmentioning

confidence: 99%

“…2, 3). Four of them could be referred to by the nomenclature of Rospars andHildebrand (1992, 2000). These were the largest of the "ordinary" glomeruli: G20 (light blue glomerulus in Figs.…”

Section: Reconstruction and Identification Of Glomerulimentioning

confidence: 99%

“…Several authors have outlined the advantages of a 3D map not only of vertebrate brains (Ruffins et al 2002; also reviewed in Van Essen et al 2002), but also of insect brain structures (Rohlfing et al 2004;Zöckler et al 2001;Chiang et al 2001;Rein et al 1999Rein et al , 2002Haddad et al 2004), especially in the AL (Drosophila melanogaster: Laissue et al 1999; Apis mellifera: Flanagan and Mercer 1989;Galizia et al 1999; Blaberus craniifer: Chambille and Rospars 1981; Mamestra brassicae : Rospars 1983; Helicoverpa assulta and Heliothis virescens: Berg et al 2002;Spodoptera littoralis: Sadek et al 2002; Agrotis ipsilon: Greiner et al 2004; Cotesia glomerata and rubecula: Smid et al 2003). The identification and 3D representation of a set of "ordinary" glomeruli in an atlas allows for their repeatedly reliable identification irrespective of the original data (Galizia et al 1999).…”

Section: Introductionmentioning

confidence: 99%

“…The identification and 3D representation of a set of "ordinary" glomeruli in an atlas allows for their repeatedly reliable identification irrespective of the original data (Galizia et al 1999). Rospars andHildebrand (1992, 2000) reconstructed ALs of adult M. sexta based on paraffin sections, but, although they provided the first map of M. sexta AL glomeruli, their work supplied only restricted information about glomerular volumes or shape. Because of the immense improvements in confocal laser scan microscopy (CLSM), computer power, and 3D reconstruction software during last few years, 3D maps have been constructed of various insect brains.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Standard three-dimensional glomeruli of the Manduca sexta antennal lobe: a tool to study both developmental and adult neuronal plasticity

Huetteroth

Schachtner

2005

Cell Tissue Res

View full text Add to dashboard Cite

The metamorphosing antennal lobe (AL) of the sphinx moth Manduca sexta serves as an established model system for studying neuronal development. To improve our understanding of mechanisms involved in neuronal plasticity, we have analyzed the size, shape, and localization of ten identified glomeruli at three different time points during development and in the adult, viz., (1) 13 days after pupal eclosion (P13), which reflects a time when the basic glomerular map has formed, (2) immediately after adult eclosion (A0), which represents a time when the newly formed glomeruli are uninfluenced by external odors, and (3) 4 days after adult eclosion (A4), which reflects a time when the animals have been exposed to surrounding odors. Our data from normally developing ALs of male M. sexta from P13 to A0 revealed an increase in size of all examined glomeruli of between 40% and 130%, with the strongest increases occurring in two of the three sex-specific glomeruli (cumulus, toroid). From A0 to A4, the cumulus and toroid increased significantly when correlated to AL volume, whereas the other glomeruli reached the sizes gained after A0. This study was based on antibody staining against the ubiquitous synaptic vesicle protein synaptotagmin, confocal laser scan microscopy, and the three-dimensional (3D) analysis tool AMIRA. Tissue permeability and therefore reliability of the staining quality was enhanced by using formalin/methanol fixation. The standard 3D glomeruli introduced in this study can now be used as basic tools for further examination of neuronal plasticity at the level of the identified neuropil structures, viz., the glomeruli of the AL of M. sexta.

show abstract

Section: Introductioncontrasting

confidence: 38%

Section: Organization Of Al Neuropilmentioning

confidence: 99%

“…2, 3). Four of them could be referred to by the nomenclature of Rospars andHildebrand (1992, 2000). These were the largest of the "ordinary" glomeruli: G20 (light blue glomerulus in Figs.…”

Section: Reconstruction and Identification Of Glomerulimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Standard three-dimensional glomeruli of the Manduca sexta antennal lobe: a tool to study both developmental and adult neuronal plasticity

Huetteroth

Schachtner

2005

Cell Tissue Res

View full text Add to dashboard Cite

show abstract

Influence of receptor axons on the formation of olfactory glomeruli in a hemimetabolous insect, the cockroachPeriplaneta americana

Salecker

Boeckh

1996

J. Comp. Neurol.

View full text Add to dashboard Cite

The embryonic development of the hemimetabolous insect Periplaneta americana requires approximately 31 days. Deafferentation experiments were used to investigate the role of ingrowing receptor axons during embryogenesis, specifically their influence 1) on the subdivision of the antennal lobe neuropil into glomeruli, 2) on the morphology and number of glial cells, and 3) on the arborization pattern of central neurons. The flagellum of one antenna was removed from embryos at different developmental stages starting with day 10. Subsequently, they were raised in culture until a total age of 26 days. At day 10, the deutocerebrum has received only a very small number (ca. 0.4%) of antennal receptor axons; deafferentation at this stage allowed us to deprive the deutocerebrum of approximately 99% of its normal antennal input. Deafferentation has marked effects on the organization of the antennal lobe neuropil. The deafferented lobe is reduced in volume compared to the control side; the characteristic glomeruli are missing. During normal development glomeruli are formed between day 19 and 22, first in dorsal and then in ventral antennal lobe regions. By removing the antenna before day 20, their formation is disturbed in all parts of the antennal lobe. If deafferentation is performed after stage 20, glomeruli persist in dorsal regions, but are missing in ventral regions. On day 24 or later, glomeruli in both dorsal and ventral regions are unaffected by deafferentation. Glial cells continue to extend fine processes into the neuropil in the absence of ingrowing receptor axons. The number of glial cells is reduced compared to control lobes. Multiglomerular local interneurons and other gamma-amino butyric acid-immunoreactive neurons, as well as projection neurons, fail to develop glomerular arborization patterns in antennal lobes deprived of sensory axons.

show abstract

Early formation of sexually dimorphic glomeruli in the developing olfactory lobe of the brain of the mothManduca sexta

1998

View full text Add to dashboard Cite

The antennal lobes (ALs), the primary olfactory centers, of the moth Manduca sexta are sexually dimorphic. Only ALs of males possess the macroglomerular complex (MGC), the site of primary processing of information about the female's sex pheromone. To understand the development of identified, odor-specific olfactory glomeruli, we investigated the cellular events involved in the morphogenesis of the MGC by means of various fluorescence staining techniques and laser-scanning confocal microscopy. The MGC lies near the entrance of the antennal nerve into the AL of the adult male and comprises three glomeruli, the globular cumulus and two toroidal structures. The MGC forms during early stages of metamorphic adult development through a stereotyped sequence of coordinated changes in MGC-specific receptor axons, glial cells, and early-ingrowing projection neurons of the medial group of AL neurons. The MGC divisions are the earliest glomeruli to form in the male AL, and their basic organization is established within about 3 days after ingrowth of the first sensory axons. Despite their special anatomical features, the MGC glomeruli develop in a manner similar to that of the ordinary glomeruli. Comparison of the ALs of males and females reveals that two relatively large and early-developing glomeruli that are situated dorsolaterally in the female AL appear to be female-specific. Development of the sexually dimorphic glomeruli diverges immediately after the ingrowth of the first olfactory receptor axons, resulting in the formation of these large glomeruli in females and the MGC in males.

show abstract

Invariance and sex‐specific variations of the glomerular organization in the antennal lobes of a moth, Mamestra brassicae, and a butterfly, Pieris brassicae

Cited by 114 publications

References 39 publications

Standard three-dimensional glomeruli of the Manduca sexta antennal lobe: a tool to study both developmental and adult neuronal plasticity

Standard three-dimensional glomeruli of the Manduca sexta antennal lobe: a tool to study both developmental and adult neuronal plasticity

Influence of receptor axons on the formation of olfactory glomeruli in a hemimetabolous insect, the cockroachPeriplaneta americana

Early formation of sexually dimorphic glomeruli in the developing olfactory lobe of the brain of the mothManduca sexta

Contact Info

Product

Resources

About