Early formation of sexually dimorphic glomeruli in the developing olfactory lobe of the brain of the mothManduca sexta

R�ssler, Wolfgang; Tolbert, Leslie P.; Hildebrand, John G.

doi:10.1002/(sici)1096-9861(19980713)396:4<415::aid-cne1>3.0.co;2-4

Cited by 35 publications

(3 citation statements)

References 55 publications

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“…In the one female investigated here, of H. virescens, two enlarged compartments were shown at the entrance of the antennal nerve, in a position corresponding to the male-specific MGC, the largest residing centrally at the antennal nerve entrance and a somewhat smaller unit in the dorsomedial position. Large female glomeruli have previously been described for the silk moth Bombyx mori (Koontz and Schneider, 1987) as well as for M. sexta (Ro ¨ssler et al, 1998). It has recently been reported that one of the two units identified in the latter mediates information specifically about the plant volatile linalool (King et al, 2000).…”

Section: Discussionmentioning

confidence: 98%

Digital atlases of the antennal lobe in two species of tobacco budworm moths, the oriental Helicoverpa assulta (male) and the American Heliothis virescens (male and female)

Berg

Galizia

Brandt

et al. 2002

J of Comparative Neurology

135

159

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The antennal lobe of the moth brain is the primary olfactory center processing information about pheromones and plant odors. We present here a digital atlas of the glomerular antennal lobe structures in the male of Helicoverpa assulta and the male and female of Heliothis virescens, based on synaptic antibody staining combined with confocal microscopy. The numbers of the glomeruli in the three specimens were similar, 65, 66, and 62, respectively. Whereas the male antennal lobe has a macroglomerular complex consisting of three and four units in the two species, the female lobe has two enlarged glomeruli at a corresponding position, near the entrance of the antennal nerve. Another large glomerulus, showing homology in the three specimens, is ventrally located. The small size of the heliothine moths is advantageous for confocal microscopy because the entire brain can be visualized as a single image stack. The maps are freely accessible on the internet, and the digital form of the data allows each atlas to be rotated and sectioned at any angle, providing for the identification of glomeruli in different preparations.

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Section: Discussionmentioning

confidence: 98%

Digital atlases of the antennal lobe in two species of tobacco budworm moths, the oriental Helicoverpa assulta (male) and the American Heliothis virescens (male and female)

Berg

Galizia

Brandt

et al. 2002

J of Comparative Neurology

135

159

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“…1). In male Manduca , the MGC exists close to the entrance of the antennal nerve into the antennal lobe and comprises three glomeruli: the cumulus, the toroid-1, and the horseshoe-shaped toroid-2 (Heinbockel et al 1995; Homberg et al 1995; Rössler et al 1998). All three glomeruli of the MGC are targeted by axonal projections of olfactory receptor cells housed in the long trichoid sensilla in the antenna as revealed by specific anterograde labeling using cobalt lysine (Christensen et al 1995) or rhodamine dextrane staining techniques (Fig.…”

Section: Organization Of the Olfactory System In Male M Sextamentioning

confidence: 99%

“…Olfactory receptor cells in trichoid type-A sensilla project their axons mainly to two large, distinct, and sexually dimorphic glomeruli, the medial and lateral large female glomerulus (medLFG, latLFG) (Rössler et al 1998, 1999; Rospars and Hildebrand 2000). Other olfactory receptor cell afferents from these sensilla project to some of the 60 sexually isomorphic glomeruli in the antennal lobe (Shields and Hildebrand 2001a).…”

Section: Organization Of the Olfactory System In Female M Sextamentioning

confidence: 99%

Glomerular interactions in olfactory processing channels of the antennal lobes

2013

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An open question in olfactory coding is the extent of interglomerular connectivity: do olfactory glomeruli and their neurons regulate the odorant responses of neurons innervating other glomeruli? In the olfactory system of the moth Manduca sexta, the response properties of different types of antennal olfactory receptor cells are known. Likewise, a subset of antennal lobe glomeruli has been functionally characterized and the olfactory tuning of their innervating neurons identified. This provides a unique opportunity to determine functional interactions between glomeruli of known input, specifically, (1) glomeruli processing plant odors and (2) glomeruli activated by antennal stimulation with pheromone components of conspecific females. Several studies describe reciprocal inhibitory effects between different types of pheromone-responsive projection neurons suggesting lateral inhibitory interactions between pheromone component-selective glomerular neural circuits. Furthermore, antennal lobe projection neurons that respond to host plant volatiles and innervate single, ordinary glomeruli are inhibited during antennal stimulation with the female’s sex pheromone. The studies demonstrate the existence of lateral inhibitory effects in response to behaviorally significant odorant stimuli and irrespective of glomerular location in the antennal lobe. Inhibitory interactions are present within and between olfactory subsystems (pheromonal and non-pheromonal subsystems), potentially to enhance contrast and strengthen odorant discrimination.

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Targeted ingrowth and glial relationships of olfactory receptor axons in the primary olfactory pathway of an insect

et al. 1998

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Olfactory receptor axons in many species terminate centrally in an array of distinct glomeruli that are thought to encode the molecular features of odors. Particular molecular attributes are detected by receptor neurons widely distributed over the sensory epithelium, but these neurons then project to a small number of glomeruli in the olfactory bulb. This raises perplexing questions about olfactory axon guidance, especially how axons sort by odor specificity and how they find their appropriate targets in the brain. Taking advantage of the relative cellular simplicity of the moth antennal system, we have examined receptor axons in normally developing animals and also in preparations in which the nerve was experimentally misrouted. Just before they enter the antennal lobe, receptor axons undergo a dramatic reorganization in a discrete zone filled with glial cells. Here they shed neighbor relationships and become associated with axons that have common targets and presumably share common odor specificities. Electron microscopy revealed that the growth cones of early arriving axons travel preferentially next to glial processes. The growth cones of receptor axons were relatively simple except as they entered newly forming glomeruli. Misrouted nerves turned and ran along the surface of the brain until they reached the region of the antennal lobe. In only 6% of cases did misrouted axons enter the brain ectopically, never forming glomeruli. Our results suggest that olfactory receptor axons are attracted to the antennal lobe by soluble or surface-bound cues and sort by odor specificity by using a mechanism that may involve glial cells.

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Early formation of sexually dimorphic glomeruli in the developing olfactory lobe of the brain of the mothManduca sexta

Cited by 35 publications

References 55 publications

Digital atlases of the antennal lobe in two species of tobacco budworm moths, the oriental Helicoverpa assulta (male) and the American Heliothis virescens (male and female)

Digital atlases of the antennal lobe in two species of tobacco budworm moths, the oriental Helicoverpa assulta (male) and the American Heliothis virescens (male and female)

Glomerular interactions in olfactory processing channels of the antennal lobes

Targeted ingrowth and glial relationships of olfactory receptor axons in the primary olfactory pathway of an insect

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