Introduction of a 20 kHz Nd:YVO4 laser into a hybrid quadrupole time-of-flight mass spectrometer for MALDI-MS imaging

Trim, Paul J.; Djidja, Marie-Claude; Atkinson, Sally; Oakes, Keith; Cole, Laura; Anderson, David M.; Hart, Philippa J.; Francese, Simona; Clench, Malcolm R.

doi:10.1007/s00216-010-3874-6

Cited by 80 publications

(64 citation statements)

References 10 publications

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“…Compared with the pixel-to-pixel mode, the continuous raster mode was nearly 10 times faster and, therefore, a similar improvement as the 6-fold gain reported by Trim et al [12] was achieved. In this way, a whole coronal mouse brain section (about 6 mm × 9 mm wide) could be imaged using a pixel size of 20 μm within 4.5 h. Interestingly, despite of the lower number of 30 versus 100 laser pulses applied, the signal-tonoise (S/N) ratios of the ion images were generally higher in rastering mode.…”

Section: Comparison Of Pixel and Continuous-raster Imagingsupporting

confidence: 75%

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Influence of the Laser Spot Size, Focal Beam Profile, and Tissue Type on the Lipid Signals Obtained by MALDI-MS Imaging in Oversampling Mode

Wiegelmann

Dreisewerd

Soltwisch

2016

J. Am. Soc. Mass Spectrom.

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Abstract. To improve the lateral resolution in matrix-assisted laser desorption/ ionization mass spectrometry imaging (MALDI-MSI) beyond the dimensions of the focal laser spot oversampling techniques are employed. However, few data are available on the effect of the laser spot size and its focal beam profile on the ion signals recorded in oversampling mode. To investigate these dependencies, we produced 2 times six spots with dimensions between~30 and 200 μm. By optional use of a fundamental beam shaper, square flat-top and Gaussian beam profiles were compared. MALDI-MSI data were collected using a fixed pixel size of 20 μm and both pixel-by-pixel and continuous raster oversampling modes on a QSTAR mass spectrometer. Coronal mouse brain sections coated with 2,5-dihydroxybenzoic acid matrix were used as primary test systems. Sizably higher phospholipid ion signals were produced with laser spots exceeding a dimension of~100 μm, although the same amount of material was essentially ablated from the 20 μm-wide oversampling pixel at all spot size settings. Only on white matter areas of the brain these effects were less apparent to absent. Scanning electron microscopy images showed that these findings can presumably be attributed to different matrix morphologies depending on tissue type. We propose that a transition in the material ejection mechanisms from a molecular desorption at large to ablation at smaller spot sizes and a concomitant reduction in ion yields may be responsible for the observed spot size effects. The combined results indicate a complex interplay between tissue type, matrix crystallization, and laser-derived desorption/ablation and finally analyte ionization.

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Section: Comparison Of Pixel and Continuous-raster Imagingsupporting

confidence: 75%

“…In their work, a 10 kHz-Nd:YVO 4 laser was used with a pixel size of 150 μm [12]. The manufacturer of the QSTAR spectrometer (AB Sciex) reported similar time-saving values in a technical note, with a possible lateral resolution in the raster-direction of about 30 μm using a laser spot size of 150 μm [13].…”

Section: Introductionmentioning

confidence: 99%

Influence of the Laser Spot Size, Focal Beam Profile, and Tissue Type on the Lipid Signals Obtained by MALDI-MS Imaging in Oversampling Mode

Wiegelmann

Dreisewerd

Soltwisch

2016

J. Am. Soc. Mass Spectrom.

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“…This is reduced in most TOF/TOF mode cases, where laser rates start at 1 kHz and reaching 2 kHz in some specific instruments. Developments with regards to laser rates have seen levels of 20 kHz being achieved (Trim et al 2010) and overall acquisition rates achievements at 109 current commercially available acquisition rates (Spraggins and Caprioli 2011) which thus highlights the drive in developments to reduce the acquisition time spend per sample analysed.…”

Section: Instrumental Developmentsmentioning

confidence: 96%

The potential of mass spectrometry imaging in plant metabolomics: a review

Heyman

Dubery

2015

Phytochem Rev

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Imaging mass spectrometry (MSI) has contributed important information to several scientific fields and more recently to the biological sciences. The use of MSI as an investigative tool for plant metabolomics has recently been explored and initial results show a promising direction that plant metabolomics could be heading to with the use of these mass directed imaging techniques. Matrix assisted laser desorption ionisation (MALDI), secondary ion mass spectrometry and desorption electrospray ionisation mass spectrometry are the best-known MSI techniques used currently. MALDI-MSI is the most common used technique for molecular imaging. With new developments, spatial resolution capabilities have reached 5 lm which has resulted in a significant improvement in the data outputs for MSI analysis. Developments in the improvement of sample preparation techniques, instrumental improvements and more efficient and effective data analysis, have had a dramatic improvement in the obtained informational content. More recent developments on instrumentation and applications are seeing a change in direction towards the imaging of lower mass range metabolites. The unique feature that MSI brings to bio-analytical analysis is the possibility to analyse the distribution of hundreds of plant metabolites across a sample without any significant sample preparation. This has highlighted the potential of MSI techniques as ideal for metabolome analysis with the additional advantage of generating crucial spatial distribution information as well. The further development of MSI for the use in plant metabolomics is at an exciting time and the prospects for interesting new discoveries are very evident.

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“…To keep measuring times to an applicable level, it is necessary to develop new ways to increase the throughput of MALDI-IMS systems. To achieve this, there has been development to increase the frequency of the used lasers, which allowed lowering the measurement time of tissue samples by up to 90% (Trim et al 2010). In addition, an automated setup was developed to save time in between experiments, which included controlled sample storage, a sample loading robot and a MALDI-TOF/TOF mass spectrometer, all controlled by a single user interface.…”

Section: Measuring Speedmentioning

confidence: 98%

MALDI imaging mass spectrometry for direct tissue analysis: technological advancements and recent applications

Balluff

Schöne

Höfler

et al. 2011

Histochem Cell Biol

111

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Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a method that allows the investigation of the molecular content of tissues within its morphological context. Since it is able to measure the distribution of hundreds of analytes at once, while being label free, this method has great potential which has been increasingly recognized in the field of tissue-based research. In the last few years, MALDI-IMS has been successfully used for the molecular assessment of tissue samples mainly in biomedical research and also in other scientific fields. The present article will give an update on the application of MALDI-IMS in clinical and preclinical research. It will also give an overview of the multitude of technical advancements of this method in recent years. This includes developments in instrumentation, sample preparation, computational data analysis and protein identification. It will also highlight a number of emerging fields for application of MALDI-IMS like drug imaging where MALDI-IMS is used for studying the spatial distribution of drugs in tissues.

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Introduction of a 20 kHz Nd:YVO4 laser into a hybrid quadrupole time-of-flight mass spectrometer for MALDI-MS imaging

Cited by 80 publications

References 10 publications

Influence of the Laser Spot Size, Focal Beam Profile, and Tissue Type on the Lipid Signals Obtained by MALDI-MS Imaging in Oversampling Mode

Influence of the Laser Spot Size, Focal Beam Profile, and Tissue Type on the Lipid Signals Obtained by MALDI-MS Imaging in Oversampling Mode

The potential of mass spectrometry imaging in plant metabolomics: a review

MALDI imaging mass spectrometry for direct tissue analysis: technological advancements and recent applications

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