Introducing of a New Experimental Method in Semen Preparation: Supernatant Product of Adipose Tissue: Derived Mesenchymal Stem Cells (SPAS)

Fazaeli, Hoda; Faeze, Davoodi

doi:10.4172/2375-4508.1000178

Cited by 2 publications

(7 citation statements)

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“…The rich content in growth factors of SPAS may be responsible for the mentioned parameters improvement. This result is in agreement with the findings of who found that this preparation method helped the isolation of spermatozoa ( 14 ). There are a number of studies investigating the growth factors role in spermatogenesis as following: In 2012 it is reported that development, maturation, and motility of the spermatozoa will be affected by variations in IGF-1 levels of seminal plasma ( 8 ).…”

Section: Discussionsupporting

confidence: 93%

“…Isolation of ATMSCs was carried out as described previously by Fazaeli et al ( 14 ). ATMSCs in 3 rd -9 th passage were used for the experiment.…”

Section: Methodsmentioning

confidence: 99%

“…In the day of IUI, defreezing of SPAS was done in a water bath at 37 o C. The total sample was divided in to 1 mL volumes in test tubes and 1.5-2 mL of SPAS was slowly added to the tube. Then the tubes were placed in the 37 o C incubator at an angel of 45 degrees for 40 min ( 14 ). Using a sterile pipette the supernatant was gently collected and finally, it was centrifuged with 5 mL of Hams F10+ 2.5% HSA at 2500g for 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, use of the supernatant product of adipose tissue derived mesenchymal stem cells (SPAS) method has been reported as a highly effective semen preparation technique improving sperm parameters including total and progressive motility as well as DNA fragmentation ( 14 ). The cause of selecting adipose tissue as MSCs source is higher secretion of some proteins involved in improving semen parameters such as basic fibroblast growth factor, interferon gamma and IGF-1 rather than bone marrow derived mesenchymal stem cells ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

“…The cause of selecting adipose tissue as MSCs source is higher secretion of some proteins involved in improving semen parameters such as basic fibroblast growth factor, interferon gamma and IGF-1 rather than bone marrow derived mesenchymal stem cells ( 15 ). Furthermore, there are some other biological advantages for adipose tissue derived mesenchymal stem cells (ATMSCs) in comparison to bone marrow derived mesenchymal stem cells including better proliferative capacity, higher immune-modulatory effects, easier accessibility to adipose source and subsequently higher number of recovered MSCs and noninvasive cell isolation of adipose tissue ( 14 , 15 ).…”

Section: Introductionmentioning

confidence: 99%

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The effect of supernatant product of adipose tissue derived mesenchymal stem cells and density gradient centrifugation preparation methods on pregnancy in intrauterine insemination cycles: An RCT

Fazaeli

Davoodi

Kalhor

et al. 2018

IJRM

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Background: One of the most important involved factors in pregnancy occurrence following intrauterine insemination (IUI) is semen sample preparation. Recently, supernatant product of adipose tissue derived mesenchymal stem cells (SPAS) method has been shown to improve semen parameters. Objective: To compare the effect of preparation methods in order to IUI, SPAS and density gradient centrifugation (DGC). Materials and Methods:This trial was done on 80 couples with male factor infertility who attend jihad daneshgahi infertility treatment center of Qom province, undergoing ovarian stimulation and IUI cycle. Various semen parameters including motility, count, DNA fragmentation and capacitation were evaluated before and after preparation. The effect of semen preparation methods and influence of various semen parameters on pregnancy occurrence were examined. Results: The overall clinical pregnancy rate was 17.5% per patient with no miscarriage. The pregnancy rate for DGC and SPAS were 5% (2 of 40) and 30% (12 of 40) respectively. Since there is no significant difference in improving motion parameters between two groups (except recovery of total number of motile spermatozoa), it seems that these parameters alone are not sufficient to predict IUI pregnancy outcome whereas in samples with >25 million motile spermatozoa in inseminate, there was a clear trend for a higher pregnancy rate for the sample processed using SPAS. Conclusion: Considering SPAS as a new and effective method leading to provide a combination of various improved semen parameters, is expected in near future.

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Section: Discussionsupporting

confidence: 93%

“…Isolation of ATMSCs was carried out as described previously by Fazaeli et al ( 14 ). ATMSCs in 3 rd -9 th passage were used for the experiment.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The effect of supernatant product of adipose tissue derived mesenchymal stem cells and density gradient centrifugation preparation methods on pregnancy in intrauterine insemination cycles: An RCT

Fazaeli

Davoodi

Kalhor

et al. 2018

IJRM

Self Cite

View full text Add to dashboard Cite

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Improvement of Human Sperm Vacuolization and DNA Fragmentation Co-Cultured with Adipose-Derived Mesenchymal Stem Cell Secretome: In Vitro Effect

Bader

Ibrahim

Mourad

et al. 2019

IJSC

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Background and ObjectivesOxidative stress (OS) is known to be an important factor of male infertility. Adipose-derived mesenchymal stem cells (AD-MSCs) are known to have immune-modulatory and anti-oxidant effects through their secretions, hence raising the idea of their potential benefit to improve sperm parameters. This study aims at investigating the effect of AD-MSCs conditioned medium (CM) on human sperm parameters in the presence and absence of H2O2-induced OS.Methods and ResultsSperm samples were collected from 30 healthy men and divided into two groups: non-stressed and H2O2-stressed. Isolated AD-MSCs from healthy donors undergoing liposuction were cultured and CM was collected at 24, 48 and 72 h. Both sperm groups were cultured with CM and a time course was performed followed by an evaluation of sperm parameters. The incubation of non-stressed and stressed sperm samples with AD-MSCs-CM for 24 h was found to have the optimum impact on sperm vacuolization, DNA fragmentation and OS levels in comparison to other incubation timings, while preserving motility, viability and morphology of cells. Incubation with CM improved all sperm parameters except morphology in comparison to the non-treated group, with the best effect noted with CM collected at 24 h rather than 48 or 72 h for sperm vacuolization and DNA fragmentation. When compared to fresh semen parameters (T0), samples cultured with CM 24 h showed a significant decrease in sperm vacuolization and DNA fragmentation while keeping other parameters stable.ConclusionsAD-MSCSs-CM improves sperm quality, and hence can be used in treating infertility and subsequently enhancing IVF outcomes.

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Introducing of a New Experimental Method in Semen Preparation: Supernatant Product of Adipose Tissue: Derived Mesenchymal Stem Cells (SPAS)

Cited by 2 publications

References 0 publications

The effect of supernatant product of adipose tissue derived mesenchymal stem cells and density gradient centrifugation preparation methods on pregnancy in intrauterine insemination cycles: An RCT

The effect of supernatant product of adipose tissue derived mesenchymal stem cells and density gradient centrifugation preparation methods on pregnancy in intrauterine insemination cycles: An RCT

Improvement of Human Sperm Vacuolization and DNA Fragmentation Co-Cultured with Adipose-Derived Mesenchymal Stem Cell Secretome: In Vitro Effect

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