The effect of supernatant product of adipose tissue derived mesenchymal stem cells and density gradient centrifugation preparation methods on pregnancy in intrauterine insemination cycles: An RCT

Abstract

Background: One of the most important involved factors in pregnancy occurrence following intrauterine insemination (IUI) is semen sample preparation. Recently, supernatant product of adipose tissue derived mesenchymal stem cells (SPAS) method has been shown to improve semen parameters. Objective: To compare the effect of preparation methods in order to IUI, SPAS and density gradient centrifugation (DGC). Materials and Methods:This trial was done on 80 couples with male factor infertility who attend jihad daneshgahi infertility treatment center of Qom province, undergoing ovarian stimulation and IUI cycle. Various semen parameters including motility, count, DNA fragmentation and capacitation were evaluated before and after preparation. The effect of semen preparation methods and influence of various semen parameters on pregnancy occurrence were examined. Results: The overall clinical pregnancy rate was 17.5% per patient with no miscarriage. The pregnancy rate for DGC and SPAS were 5% (2 of 40) and 30% (12 of 40) respectively. Since there is no significant difference in improving motion parameters between two groups (except recovery of total number of motile spermatozoa), it seems that these parameters alone are not sufficient to predict IUI pregnancy outcome whereas in samples with >25 million motile spermatozoa in inseminate, there was a clear trend for a higher pregnancy rate for the sample processed using SPAS. Conclusion: Considering SPAS as a new and effective method leading to provide a combination of various improved semen parameters, is expected in near future.

Improvement of Human Sperm Vacuolization and DNA Fragmentation Co-Cultured with Adipose-Derived Mesenchymal Stem Cell Secretome: In Vitro Effect

“…The significant improvement in sperm parameters may be attributed to the numerous secreted bioactive factors mainly growth factors VEGF, granulocyte-colony stimulating factor (G-CSF), IGF-1, HGF, NGF, and FGF (13), anti- inflammatory cytokines IL-10 and transforming Growth Factor- β (TGF- β ) (13), and antioxidant defense constituents, more particularly superoxide dismutase (SOD) and catalase (20). Actually it was reported that VEGF, HGF, FGF2, NGF and IGF-1 were capable of maintaining and improving sperm motility by stabilizing sperm membrane proteins (28–31).…”

“…As a promising therapy that warrants further investigation, the biological effects of CM of AD-MSCs on sperm quality were recently evaluated. However, methods of isolation and culture of AD-MSCs, and procedures of preparation of CM were not standardized, thus leading sometimes to inconclusive results (12, 13). Therefore, the aim of this study was to investigate the effect of AD-MSCs-CM on human sperm parameters, where oxidative stress levels and sperm vacuolization are being investigated for the first time.…”