Introducing a novel chemotherapeutic drug formulated with anthraflavic acid for treating human breast carcinoma and type 2 diabetes mellitus

Abstract

IntroductionMolecular docking as a versatile theoretical method was used to investigate the biological activities of anthraflavic acid in the presence of alpha amylase. The outcomes revealed that anthraflavic acid has a considerable binding affinity to the enzyme with a docking score of -7.913 kcal/mol.Material and methodsThese outcomes were further evaluated with free binding energy calculations, and it was concluded that anthraflavic acid could be a potential inhibitor for alpha amylase. The as Anthraflavic acid was explored in the anti-human breast carcinoma tests. The in vitro cytotoxic and anti-breast carcinoma effects of biologically synthesized Anthraflavic acid against MCF-7, CAMA-1, SK-BR-3, MDA-MB-231, AU565 [AU-565], and Hs 281.T cancer cell lines were assessed.ResultsThe anti-breast carcinoma properties of the Anthraflavic acid could significantly remove MCF-7, CAMA-1, SK-BR-3, MDA-MB-231, AU565 [AU-565], and Hs 281.T cancer cell lines in a time and concentration-dependent manner by MTT assay.ConclusionsThe IC50 of the Anthraflavic acid were 159, 193, 253, 156, 241, and 218 µg/mL against MCF-7, CAMA-1, SK-BR-3, MDA-MB-231, AU565 [AU-565], and Hs 281.T cancer cell lines. It seems that the anti-human breast carcinoma effect of recent nanoparticles is due to their antioxidant effects.After clinical study, Anthraflavic acid can be utilized as an efficient drug in the treatment of breast carcinoma in humans.

One‐step synthesis of silver nanostructures using Heracleum persicum fruit extract, their cytotoxic activity, anti‐cancer and anti‐oxidant activities

“…After centrifuging the sample at 5000 rpm for 5 min, trypsin culture medium was added to decrypt the cell precipitate. Subsequently, trypan blue dye was added, the cell suspension was counted on a Neobar slide and cytotoxicity was conducted by MTT method [19,20].…”

“…Hs 281.T, MDA-MB-468, AU565 [AU-565], MCF7, CAMA-1, SK-BR-3, NMU, and RBA is because of their antiox- idant impacts. Whereas tumor advancement is firmly related to inflammation and OS, compounds with antioxidant or antiinflammatory properties have the potential to be anti-cancer agents [20,29,30,[33][34][35][36][37][38][39][40]50].…”