1969
DOI: 10.1002/bip.1969.360070511
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Intrinsic viscosity of native and single‐stranded T7 DNA and its relationship to sedimentation coefficient

Abstract: The intrinsic viscosity and sedimentation coefficient of native and single-stranded T7 DNA have been determined at 25⁰C as a function of ionic strength in neutral and alkaline NaCl. The relationship between [η] and S⁰(20,w), is well represented by the Mandelkern-Flory equation over the entire range of conditions between 0.0013 and 1M Na+. An apparent discrepancy between the two methods at moderate to high ionic strengths is probably due to a change in V with ionic strength. It appears that [η] is a more sensit… Show more

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Cited by 91 publications
(21 citation statements)
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“…The value obtained from the extrapolation to saturation of the electric orientation (2) is 30% higher (gl - A possible explanation for the comparatively high value found in fibers and films might be related to the difference in the internal field correction: from our definition of Aao, which incorporates the anisotropy of the internal field and which is the measured quantity via Eqs. (7) and (8), it follows that Lao = fallEl1 -aiEL)/Eo with a11 and a 1 being the intrinsic polarizabilities; Eo is the incident electromagnetic field, and Ell and E L the internal field for Eo directed along and perpendicular to the helix axis. It is known from the treatment of hypochromicity as a local field effect30 that in solution, E I < ,311, so that, since la11 > IqI,…”
Section: Optical Anisotropy Factor Aaomentioning
confidence: 99%
“…The value obtained from the extrapolation to saturation of the electric orientation (2) is 30% higher (gl - A possible explanation for the comparatively high value found in fibers and films might be related to the difference in the internal field correction: from our definition of Aao, which incorporates the anisotropy of the internal field and which is the measured quantity via Eqs. (7) and (8), it follows that Lao = fallEl1 -aiEL)/Eo with a11 and a 1 being the intrinsic polarizabilities; Eo is the incident electromagnetic field, and Ell and E L the internal field for Eo directed along and perpendicular to the helix axis. It is known from the treatment of hypochromicity as a local field effect30 that in solution, E I < ,311, so that, since la11 > IqI,…”
Section: Optical Anisotropy Factor Aaomentioning
confidence: 99%
“…Equation The electrostatic excluded volume Pel between effective Kuhn segments can be reasonably approximated by4 Pel = 2xL;d (6) Here d is the effective diameter of a Kuhn segment, and it has recently been calculated by Fixman and Skolnick'O: (7) d N K -' (- where [7]0 is the value of the intrinsic viscosity extrapolated to infinite salt concentration, (Lt/Lp)3'2 signifies the hypothetical theta-state expansion: and a,, is a complicated function of a s Weill and des Cloizeaux5 derive a relation for a? which seems to be in better agreement with experiments on uncharged polymers than other theories."…”
Section: On the Ionic-strength Dependence O F The Intrinsic Viscositymentioning
confidence: 99%
“…For example, due to the negative charge of the DNA, changes in the ionic strength of the solvent affect the conformation of the DNA molecule and therefore both its sedimentation coefficient (s) and its intrinsic viscosity (v). As s and 1 vary with ionic strength they remain related over a wide range of ionic strengths as described by the Mandelkern and Flory equation for both native and single-stranded T7 DNA in alkaline or neutral solutions (37). Therefore, the size determinations of mammalian DNA by alkaline sucrose gradients (10,25,26), neutral sucrose gradients (17)(18)(19), and viscoelastometry (20,24) are based on related parameters that can be similarly affected by changes in the hydrodynamic properties of the DNA molecule.…”
Section: Introductionmentioning
confidence: 99%