Intrinsic Neuroprotection in Traumatic Brain Injury

Shohami, Esther; Horowitz, Michal

doi:10.1007/978-1-4419-9695-4_23

Cited by 1 publication

(1 citation statement)

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“…Upregulation of muscarinic receptors and regulate the fatty acid environment of these receptors and other proteins yield a key means in protecting their integrity during thermal stress, and these pathways are further involved in other regulatory mechanisms (Shmeeda et al, 2002). These lipid mediators activate their intracellular signaling cascades that promote cell survival through their specific receptors and coupling mechanisms (Shohami and Horowitz, 2013). Therefore, regulation of lipid/lipoprotein metabolism is an important pathway for the regulation of HA.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats

Fan

Wang

et al. 2019

Front. Physiol.

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Circular RNAs (circRNAs) have vital roles in great variety of biological processes. However, expression levels and functions of circRNAs related to heat acclimation (HA) are poorly understood. This study is the first time an in-depth circRNA expression profiling were used to investigate circRNA–miRNA interactions in HA rats in order to further comprehend the mechanisms underlying HA. CircRNA expression profile was performed in rats’ hypothalamus of HA and control group with microarray assays and their functions were predicted by using Bioinformatics analysis. Differential circRNAs and their regulated downstream miRNAs and mRNAs were quantitatively validated by means of quantitative polymerase chain reaction in real-time (RT-qPCR). Enzyme-linked immunosorbent assay (ELISA) was then applied to predict the expression of proteins. In total, 53 circRNAs were expressed distinctively between the HA and Control; up- and down-regulation of circRNAs were 28 and 25, respectively, in HA (fold change > 1.5, P < 0.05). Three circRNAs and two miRNAs and three predicted mRNAs were obviously regulated after validated by RT-qPCR in HA rats. Two proteins expression were proportional to their mRNA changes. Further analysis demonstrates that circRNAs closest to HA can be categorized into three signal pathways: including rno_circRNA_014301-vs-rno-miR-3575-vs- Hif-1 α, rno_circRNA_014301-vs-rno-miR-3575-vs- Lppr4 , and rno_circRNA_010393-vs-rno-miR-20b-3p-vs- Mfap4 in hypoxia response pathways, substance/energy metabolism, and inflammatory response pathways. Our findings implicate that many circRNAs regulate expressions of genes that interact with each other to exert their functions during HA.

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Section: Discussionmentioning

confidence: 99%