Intravital microscopy of localized stem cell delivery using microbubbles and acoustic radiation force

Kokhuis, Tom J. A.; Skachkov, Ilya; Naaijkens, B.A.; Juffermans, Lynda J.M.; Kamp, Otto; Kooiman, Klazina; Steen, Antonius F.W. van der; Versluis, Michel; Jong, Nico de

doi:10.1002/bit.25337

Cited by 34 publications

(27 citation statements)

References 52 publications

(54 reference statements)

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“…This technique was also successfully used to improve the delivery of stem cells to the vessel wall which can be used for the repair of damaged tissue ( Figure 1C) by developing echogenic complexes by conjugating tMBs to stem cells (StemBells) [62].…”

Section: Techniques To Enhance Bindingmentioning

confidence: 99%

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Rooij

Daeichin

Skachkov

et al. 2015

International Journal of Hyperthermia

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Ultrasound contrast agents (UCAs) are used routinely in the clinic to enhance contrast in ultrasonography. More recently, UCAs have been functionalised by conjugating ligands to their surface to target specific biomarkers of a disease or a disease process. These targeted UCAs (tUCAs) are used for a wide range of pre-clinical applications including diagnosis, monitoring of drug treatment, and therapy. In this review, recent achievements with tUCAs in the field of molecular imaging, evaluation of therapy, drug delivery, and therapeutic applications are discussed. We present the different coating materials and aspects that have to be considered when manufacturing tUCAs. Next to tUCA design and the choice of ligands for specific biomarkers, additional techniques are discussed that are applied to improve binding of the tUCAs to their target and to quantify the strength of this bond. As imaging techniques rely on the specific behaviour of tUCAs in an ultrasound field, it is crucial to understand the characteristics of both free and adhered tUCAs. To image and quantify the adhered tUCAs, the state-of-the-art techniques used for ultrasound molecular imaging and quantification are presented. This review concludes with the potential of tUCAs for drug delivery and therapeutic applications.

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Section: Techniques To Enhance Bindingmentioning

confidence: 99%

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Rooij

Daeichin

Skachkov

et al. 2015

International Journal of Hyperthermia

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show abstract

“…Difficulties of this technique are the required particle size homogeneity for reliable results, agglomeration issues, and high system noise (Pablico-Lansigan et al 2013). The greatest advantages of this image modality is the use of non-ionizing radiation, better sensitivity than MRI, possible single-cell detection, and faster imaging speed for real-time imaging (Knopp and Buzug 2012).…”

Section: Mpimentioning

confidence: 99%

“…Only nanoparticles located in the FFP are not magnetically saturated and can be detected. Through scanning the target tissue, a spatial distribution of magnetic particles can be created (Borgert et al 2012;Gleich and Weizenecker 2005;Goodwill and Conolly 2010;Goodwill et al 2009;Knopp and Buzug 2012;Sattel et al 2009;Weizenecker et al 2009). This technique is limited to magnetic tracers and is mostly used with SPIO nanoparticles as labeling reagents (Ittrich et al 2013;Saritas et al 2013).…”

Section: Mpimentioning

confidence: 99%

Lost signature: progress and failures in in vivo tracking of implanted stem cells

Haar

Lavrentieva

Stahl

et al. 2015

Appl Microbiol Biotechnol

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Stem cell therapy as a part of regenerative medicine provides promising approaches for the treatment of injuries and diseases. The increasing use of mesenchymal stem cells in various medical treatments created the demand for long-term in vivo cell tracking methods. Therefore, it is necessary to analyze post-transplantational survival, biodistribution, and engraftment of cells. Furthermore, stem cell treatment has been discussed controversially due to possible association with tumor formation in the recipient. For therapeutic purpose, stem cells must undergo substantial manipulation such as differentiation and in vitro expansion, and this can lead to the occurrence of genetic aberrations and altered expression of both tumor suppression and carcinogenic factors. To control therapy, it is necessary to find a reliable and general method to track and identify implanted cells in the recipient. This is especially challenging for autologous transplantations, as standard fingerprinting methods cannot be applied. An optimal technique for in vivo cell monitoring does not yet exist, and its development holds several challenges: small numbers of transplanted cells, possibility of cell number quantification, minimal transfer of the contrast agent to non-transplanted cells, and no genetic modification. This review discusses most of the proposed solutions, including magnetic resonance imaging, magnetic particle imaging, positron emission tomography, single-photon emission computed tomography, and optical imaging methods. Additionally, the recent research on cell labeling for stem cell monitoring after transplantation including in vitro, ex vivo, and in vivo imaging studies is described. Promising future imaging modalities for stem cell monitoring after transplantation are shown.

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“…Radiation force was also applied to the non-invasive manipulation of stem cell-bubbles clusters in vitro and in an in vivo CAM (chick embryo chorioallantoic membrane) model to increase their attachment to the vessel wall at the desired location. 165 These complexes could be pushed without damage at velocities up to 0.25 mm/s using a pressure of 450 kPa with an off-resonance 1 MHz acoustic wave.…”

Section: Acoustic Excitationmentioning

confidence: 99%

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

et al. 2016

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Besides their use as contrast agents for ultrasound imaging, microbubbles are increasingly studied for a wide range of therapeutic applications. In particular, their ability to enhance the uptake of drugs through the permeabilization of tissues and cell membranes shows great promise. In order to fully understand the numerous paths by which bubbles can interact with cells and the even larger number of possible biological responses from the cells, thorough and extensive work is necessary. In this review, we consider the range of experimental techniques implemented in in vitro studies with the aim of elucidating these microbubble-cell interactions. First of all, the variety of cell types and cell models available are discussed, emphasizing the need for more and more complex models replicating in vivo conditions together with experimental challenges associated with this increased complexity. Second, the different types of stabilized microbubbles and more recently developed droplets and particles are presented, followed by their acoustic or optical excitation methods. Finally, the techniques exploited to study the microbubble-cell interactions are reviewed. These techniques operate over a wide range of timescales, or even off-line, revealing particular aspects or subsequent effects of these interactions. Therefore, knowledge obtained from several techniques must be combined to elucidate the underlying processes. V C 2016 AIP Publishing LLC.

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Intravital microscopy of localized stem cell delivery using microbubbles and acoustic radiation force

Cited by 34 publications

References 52 publications

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Lost signature: progress and failures in in vivo tracking of implanted stem cells

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

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