Intravital imaging of liposome behavior upon repeated administration: A step towards the development of liposomal companion diagnostic for cancer nanotherapy

Naumenko, Victor; Vodopyanov, Stepan S.; Vlasova, Kseniya Yu.; Potashnikova, Daria; Melnikov, Pavel; Vishnevskiy, Daniil A.; Garanina, Anastasiia S.; Valikhov, Marat P.; Lipatova, Anastasiya V.; Чехонин, В. П.; Majouga, Alexander G.; Abakumov, Maxim A.

doi:10.1016/j.jconrel.2020.12.014

Cited by 13 publications

(16 citation statements)

References 65 publications

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“…The intravital microscopy imaging results supported our 3D imaging analysis. Other researchers investigating nanoparticle interactions with tumor blood vessels show similar nanoparticle clusters along the vessels with liposomes ,− and polymeric nanoparticles, suggesting that our findings could be universal for different particle designs, sizes, and tumor types.…”

Section: Resultssupporting

confidence: 67%

Specific Endothelial Cells Govern Nanoparticle Entry into Solid Tumors

et al. 2021

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The successful delivery of nanoparticles to solid tumors depends on their ability to pass through blood vessels and into the tumor microenvironment. Here, we discovered a subset of tumor endothelial cells that facilitate nanoparticle transport into solid tumors. We named these cells nanoparticle transport endothelial cells (N-TECs). We show that only 21% of tumor endothelial cells located on a small number of vessels are involved in transporting nanoparticles into the tumor microenvironment. N-TECs have an increased expression of genes related to nanoparticle transport and vessel permeability compared to other tumor endothelial cells. The N-TECs act as gatekeepers that determine the entry point, distribution, cell accessibility, and number of nanoparticles that enter the tumor microenvironment.

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Section: Resultssupporting

confidence: 67%

Specific Endothelial Cells Govern Nanoparticle Entry into Solid Tumors

et al. 2021

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“…In this regard, the use of specific antibodies can be used for the staining of specific cells and further elucidate interactions between NPs and immune cells. [ 27 ]…”

Section: Resultsmentioning

confidence: 99%

“…In this regard, the use of specific antibodies can be used for the staining of specific cells and further elucidate interactions between NPs and immune cells. [27] Several recent preclinical studies have uncovered pivotal roles for cells of the immune system in nanodrug delivery and pharmacodynamics as well. "NP hitchhiking" with neutrophils from the circulation into inflamed and malignant lesions has been observed and allowed for treatment of these conditions.…”

Section: Resultsmentioning

confidence: 99%

Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes

et al. 2021

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Active-targeting nanomedicine formulations have an intricate in vivo behavior. Nanomedicines developed to target endothelial v 3 -integrin are recently demonstrated to display extensive uptake by circulating phagocytes. These phagocytes show inherent tumor-homing capacities and therefore are capable of actively delivering the endocytosed nanomaterial in lesions. Here, the targeting kinetics and mechanisms of cyclic arginine-glycine-aspartate (cRGD)-decorated lipid nanoparticles (NPs) toward activated vasculature in inflamed lesions during wound healing are studied. The cRGD-NP targeting toward inflamed lesions is identified to be mechanistically similar to the NP accumulation in cancerous lesions. Through a complementary experimental approach, it is observed that circulating phagocytes engage cRGD-NPs and are subsequently homed to the inflamed endothelium. The inflammation-associated phagocytes remain static among endothelial cells upon targeting, resulting in the extensive presence of cRGD-NP-positive phagocytes in the angiogenic vessels. Hence, phagocytic immune cells contribute to cRGD-NP targeting toward angiogenesis. This mechanistic study underlines the need for detailed investigations of NP in vivo behavior. This is critically important for the realization of NPs potential as advanced (immunological) therapeutic agents.

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“…HT-29 metastasis liver cancer cells and HT-29 cells do exhibit significant levels of the folate receptor. It is well-known that magnetic nanocomposites have enormous promise in the domains of medication delivery, cancer diagnosis and therapy, and cancer diagnostics and treatment (Ding et al., 2020 ; Kim et al., 2020 ; Naumenko et al., 2021 ). According to a recent study, iron oxide magnetite nanocomposites (MNPs) have been broadly used in pharmaceutical fields because of their high biocompatibility and low toxicity.…”

Section: Introductionmentioning

confidence: 99%

Synergic fabrication of multifunctional liposomes nanocomposites for improved radiofrequency ablation combination for liver metastasis cancer therapy

Zhang

et al. 2022

Drug Delivery

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The field of biomedical research has recently been interested in nanoplatforms with various functionalities, such as cancer drug carriers and MRI and optical imaging, as well as thermal treatment, among other things. As a result of the present investigation, a unique multifunctional liposome (MFL) was established in this investigation. Using radiofrequency-induced imaging and drug release based on magnetic field impact, a dual drug delivery targeted with tumor multi-mechanism treatment was made more effective. The C60 (fullerene) surface was coated with iron nanocomposites to establish the proposed nanosystems, and PEGylation was used (Fe 3 O 4 -C60-PEG 2000 ). For fullerene radiofrequency-triggered drug release, thermosensitive DPPC liposomes with folate-DSPE-PEG 2000 enveloped the binary nanosystems and doxorubicin (DOX). The in vitro cytotoxicity of the nanocomposites was confirmed by the liver metastasis in HT-29 colon cancer cells using radiofrequency. The flow cytometry analysis confirmed the apoptosis cell death mechanism. The thermal treatment combined chemotherapeutic MFL nano framework transformed radiofrequency radiation from thermoresponsive liposomes, which was noticed both in vivo and in vitro . Due to their superior active tumor targeting and magnetic targeting characteristics, the MFL could also selectively destroy cancerous liver cells in highly co-localized targets.

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Intravital imaging of liposome behavior upon repeated administration: A step towards the development of liposomal companion diagnostic for cancer nanotherapy

Cited by 13 publications

References 65 publications

Specific Endothelial Cells Govern Nanoparticle Entry into Solid Tumors

Specific Endothelial Cells Govern Nanoparticle Entry into Solid Tumors

Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes

Synergic fabrication of multifunctional liposomes nanocomposites for improved radiofrequency ablation combination for liver metastasis cancer therapy

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