Intravenous administration of human neural stem cells induces functional recovery in Huntington's disease rat model

Brain Transplantation of Immortalized Human Neural Stem Cells Promotes Functional Recovery in Mouse Intracerebral Hemorrhage Stroke Model

“…Transplantation of renewable, homogenous, multipotent, and well-characterized NSCs into the damaged nervous system tissues should replace lost cells and restore damaged function. Previous studies have shown that the F3 human NSCs injected intravenously or transplanted in cerebrum significantly reduced motor and neurological deficits in animal models of Parkinson's disease [19], Huntington's disease [20,21], brain ischemia/ICH [14 -16], and lysosomal storage disease [22]. The stable immortalized human NSC lines described here can be expanded readily and provide a renewable and homogeneous population of neuronal and glial cells, and they will provide most valuable means for future studies of fundamental questions in developmental neurobiology, cell and gene therapies, and research and development of new drugs and new treatment.…”

“…Previously, we have isolated a clonal human neural stem cell line (HB1.F3) that had been immortalized by a retroviral vector encoding the v-myc oncogene [5,8], and this stable human cell line shows multipotent capacity to differentiate into neurons and glial cells [8,18] and ameliorate neurological deficits in animal models of stroke [14 -16], Parkinson's disease [19], Huntington's disease [20,21], and lysosomal storage disease [22] following their transplantation into the brain. In the present study, after transplantation into the brain of ICH mice, transplanted human NSCs were found to migrate extensively from the site of implantation into other anatomical sites and to differentiate into neurons and glial cells and improve functional deficits.…”

Adult human mesenchymal cells proliferate and migrate in response to chemokines expressed in demyelination

“…Intravenous delivery of bone marrow-derived cells including MSCs 1 is of functional benefit in animal models of neurological disease, including demyelination. [2][3][4][5][6] The mechanisms by which these cells affect lesion repair are unknown, as are the means by which circulating MSCs enter lesions.…”

Noninvasive method of immortalized neural stem-like cell transplantation in an experimental model of Huntington's disease

“…Therapeutic benefits have been reported after the intravenous injection of human umbilical cord blood or bone marrow stroma cells in experimental stroke models, and contralateral grafts of conditionally immortalized neuroepithelial stem cells resolved stroke deficits (Veizovic et al, 2001). In the quinolinic acidinduced HD model, those rats with intravenous NSC transplantation reported better functional improvement in apomorphineinduced rotation test (Lee et al, 2005). Moreover, the intraventricular transplantation of fetal human brain cells was integrated into embryonic rat brain (Brüstle et al, 1998), suggesting that in addition to direct brain parenchymal transplantation, other routes of administration are possible to facilitate recovery from functional deficit in the experimental rat HD model.…”