Intrauterine stress induces bone loss in adult offspring of C3H/HeJ mice having high bone mass phenotype but not C57BL/6J mice with low bone mass phenotype

Raygorodskaya, Maria; Gabet, Yankel; Shochat, Chen; Kobyliansky, Eugene; Torchinsky, Arkady; Karasik, David

doi:10.1016/j.bone.2016.04.004

Cited by 3 publications

(11 citation statements)

References 20 publications

(38 reference statements)

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“…We previously reported that the skeletal response to intrauterine stress in the 3- and 6-mo-old offspring's femora differed by strain ( Raygorodskaya et al, 2016 ). In-utero exposure to 5-AZA, resulted in loss of bone quality in 6-mo-old C3H offspring.…”

Section: Discussionmentioning

confidence: 99%

“…Eight-week-old C57BL/6JRccHsd (C57) and C3H/HeJ (C3H) mice were obtained from Envigo (formerly Harlan Laboratories), Jerusalem, Israel ( Raygorodskaya et al, 2016 ). Standard procedures were used for animal maintenance, feeding and breeding.…”

Section: Methodsmentioning

confidence: 99%

“…At 3 and 6 mo of age, male and female offspring of treated and control mice were selected randomly for morphometric analysis using ex vivo μCT. Analyses of 3- and 6-mo old mice were reported ( Raygorodskaya et al, 2016 ). The rest of the offspring were maintained until 12 months of age and then sacrificed.…”

Section: Methodsmentioning

confidence: 99%

“…μCT analysis was performed using μCT50 system (Scanco Medical AG, Brüttisellen, Switzerland) as described in ( Raygorodskaya et al, 2016 ). In the entire intact femur we measured volumetric bone mineral density (vBMD) and axial length using the calibrated μCT software (Scanco, uct_evaluation v6.5-3).…”

Section: Methodsmentioning

confidence: 99%

“…In response to environmental stresses, early life compensatory mechanisms are critical for establishing skeletal strength ( Jepsen, 2009 ). We previously reported that strain genetics influences the response to 5-deoxy-2′-cytidine (5-AZA) in C3H/HeJ (C3H) and C57Bl/6J (C57) mice and contemplated about the potential mechanisms of strain and sex effects observed in young/adult animals ( Raygorodskaya et al, 2016 ). Our choice of 5-AZA was dictated by the fact that teratological studies in mice have revealed that 5-AZA injected on day 10 of gestation induces phocomelia (absence of the long bones of the limb) in a dose-dependent fashion ( Torchinsky et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the long-term skeletal effect induced by teratogen 5-aza-2′deoxycytidine on offspring of high (C3H/HeJ) and low (C57BL/6J) bone mass phenotype mice

et al. 2018

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The long term skeletal effects of antenatal exposure to teratogen 5-deoxy-2′-cytidine (5-AZA) were studied using two inbred strains, C3H/HeJ (C3H, with inherently stronger bones) and C57Bl/6J (C57, with weaker bones). We previously reported that in-utero exposure to 5-AZA resulted in loss of bone quality in 3- and 6-mo-old C3H offspring. In this study, we further examined whether the long-term effects of an acute teratogenic exposure are still evident in older mice. Bone phenotypes of 12 mo-old mice exposed to a single injection of 5-AZA on day 10 of their mother's pregnancy were evaluated by micro-computed tomography and compared to the untreated controls.The main observation of this study is that 5-AZA-induced loss of bone length was registered in 12-mo-old C57 and C3H males. As expected, we did not find differences in the 3rd lumbar vertebra since in-utero exposure to 5-AZA was shown to affect the limb buds but not the axial skeleton. Trajectory of changes in bone phenotypes from ages 3 mo through 6 mo to 12 mo was also compared; 5-AZA-exposed C57 males had consistently lower femoral length and trabecular BMD than age-matched controls. In summary, by characterizing teratogen-exposed C57 and C3H mice, we further confirmed that the adaptive response to antenatal insults continue into mid-life of the mice as well as there is a sex-specificity of these responses.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%