Intrauterine exosomes are required for bovine conceptus implantation

Kusama, Kaoru; Nakamura, Keigo; Bai, Rulan; Nagaoka, Kentaro; Sakurai, ﻿Toshihiro; Imakawa, Kazuhiko

doi:10.1016/j.bbrc.2017.11.176

Cited by 83 publications

(106 citation statements)

References 35 publications

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“…In addition to that, endometrial cells treated with EVs from the post-implantation induced and increase in vascular cell adhesion molecule 1 (VCAM) transcript, indicating the modulation of adhesion molecules [70]. Furthermore, exosomes isolated from uterine flushing obtained from pregnant cows on days 17, 20, and 22 were used to treat trophoblast CT-1 cells and did not induce changes in IFNT and CDX2 mRNA expression, suggesting that the pregnancy period may influence EVs' contents [70]. Together, this information highlights the EVs biological role during the period of maternal recognition of pregnancy, which may enhance embryo-maternal communication and consequently contribute to the maintenance of pregnancy.…”

Section: Embryo-maternal Interactions Between Uterus and Embryo Mediamentioning

confidence: 95%

“…Besides that, EVs isolated from uterine flushing in the pre-implantation period increase the expression of apoptotic genes (BAX, CASP3, TNFA, and TP53 transcripts) in endometrial cells [70]. In addition to that, endometrial cells treated with EVs from the post-implantation induced and increase in vascular cell adhesion molecule 1 (VCAM) transcript, indicating the modulation of adhesion molecules [70]. Furthermore, exosomes isolated from uterine flushing obtained from pregnant cows on days 17, 20, and 22 were used to treat trophoblast CT-1 cells and did not induce changes in IFNT and CDX2 mRNA expression, suggesting that the pregnancy period may influence EVs' contents [70].…”

Section: Embryo-maternal Interactions Between Uterus and Embryo Mediamentioning

confidence: 99%

“…In addition to that, sheep endometrial epithelium can secrete exosomes containing ovine endogenous jaagsiekte retroviruses (enJSRV) mRNA, which acts on trophectoderm via toll-like receptors (TLR) to induce IFNT production [69]. Trophoblast cells from the conceptus at day 15 and 17 secrete EVs containing IFNT that are able to stimulate ISGs' expression in endometrial cell culture [22,70]. Furthermore, macrophage-capping protein (CAPG) and aldo-keto reductase family 1, member B1 protein (AKR1B1) proteins are present in EVs isolated from the uterine flushing of pregnant cows on days 15 and 17 of gestation [22].…”

Section: Embryo-maternal Interactions Between Uterus and Embryo Mediamentioning

confidence: 99%

“…Furthermore, macrophage-capping protein (CAPG) and aldo-keto reductase family 1, member B1 protein (AKR1B1) proteins are present in EVs isolated from the uterine flushing of pregnant cows on days 15 and 17 of gestation [22]. Besides that, EVs isolated from uterine flushing in the pre-implantation period increase the expression of apoptotic genes (BAX, CASP3, TNFA, and TP53 transcripts) in endometrial cells [70]. In addition to that, endometrial cells treated with EVs from the post-implantation induced and increase in vascular cell adhesion molecule 1 (VCAM) transcript, indicating the modulation of adhesion molecules [70].…”

Section: Embryo-maternal Interactions Between Uterus and Embryo Mediamentioning

confidence: 99%

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Extracellular Vesicles Mediated Early Embryo–Maternal Interactions

Bridi

Perecin

Silveira

2020

IJMS

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Embryo-maternal crosstalk is an important event that involves many biological processes, which must occur perfectly for pregnancy success. This complex communication starts from the zygote stage within the oviduct and continues in the uterus up to the end of pregnancy. Small extracellular vesicles (EVs) are part of this communication and carry bioactive molecules such as proteins, lipids, mRNA, and miRNA. Small EVs are present in the oviductal and uterine fluid and have important functions during fertilization and early embryonic development. Embryonic cells are able to uptake oviductal and endometrium-derived small EVs. Conversely, embryo-derived EVs might modulate oviductal and uterine function. In this review, our aim is to demonstrate the role of extracellular vesicles modulating embryo-maternal interactions during early pregnancy.2 of 15 luteotrophic and anti-luteolytic actions, guaranteeing the corpus luteum (CL)'s capability to secrete the progesterone necessary to establish and sustain pregnancy [15,16]. Additionally, different biological molecules are secreted by the embryos of several mammalian species during MRP as interferon-tau (IFNT) in ruminant [17], estradiol in pigs [18], and chorionic gonadotropin in humans [19]. However, recent reports indicate that the embryo is also able to secrete EVs [20][21][22], but their role in MRP is still elusive.Therefore, in mammals, the embryos and the female tract (oviduct and endometrium) are able to secrete extracellular vesicles during the pre-implantation period. Extracellular vesicles are cell-secreted vesicles that are classified according the their size, biogenesis, and secretion, as exosomes, microvesicles, and apoptotic bodies [23]. Several cellular types can secrete EVs including follicular [24,25], oviductal, and endometrial cells [13,21,26], as well as in vitro and in vivo produced embryos [20][21][22]27]. Extracellular vesicles carry bioactive molecule as miRNAs, mRNAs [28], proteins [29], and lipids [30], which modulate various reproductive processes such as ovarian follicular development, oocyte maturation, embryonic development, maternal-embryonic communication, and the establishment of mammalian gestation. There is experimental evidence that EVs participate in intercellular communication in essential reproductive events related to the establishment of pregnancy, such as cell proliferation, crosstalk between the maternal organism and conceptus, as well as during embryonic implantation [31]. In early stages of pregnancy, the communication between the conceptus and maternal organism is necessary and the evidence of the participation of EVs of maternal or embryonic origin is increasing [14].Herein, we will review the current knowledge on embryo-maternal interactions, with special emphasis on the roles of EVs during the early crosstalk between embryonic and maternal tissues.Int. J. Mol. Sci. 2020, 21, 1163 3 of 15 (reviewed by [47]). PGE2 secreted by equine viable embryos during early embryonic development is involved with the initial oviductal transpo...

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Section: Embryo-maternal Interactions Between Uterus and Embryo Mediamentioning

confidence: 95%

Section: Embryo-maternal Interactions Between Uterus and Embryo Mediamentioning

confidence: 99%

Section: Embryo-maternal Interactions Between Uterus and Embryo Mediamentioning

confidence: 99%

Section: Embryo-maternal Interactions Between Uterus and Embryo Mediamentioning

confidence: 99%

See 2 more Smart Citations

Extracellular Vesicles Mediated Early Embryo–Maternal Interactions

Bridi

Perecin

Silveira

2020

IJMS

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“…The microvesicle-like particles are present in saliva, plasma, milk, urine, semen, uterine luminal fluid and other biological fluids [11][12][13][14]. Moreover, these microvesicles have been isolated and characterized from the ULF in human [15], mice [16], cattle [17,18], and sheep [19]. However, porcine ULF exosomes have not been isolated and identified so far.…”

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confidence: 99%

Small RNA-seq analysis of exosomes from porcine uterine luminal fluid during peri-implantation

Hua

Wang

Lian

et al. 2019

Preprint

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Background The exosomes of uterine luminal fluid (ULF) mediate intrauterine communication between conceptus and uterus in pigs. The small RNAs of ULF exosomes are widely recognized as important factors that influence embryonic implantation in humans, mice, cattle and sheep. Therefore, it can be hypothesized that small RNAs of ULF exosomes are essential for implantation in pigs. Results Cupshaped exosomes (diameters: 40-160 nm) of porcine ULF were isolated and characterized, and then PKH67 stained exosomes were taken up by endometrial epithelial cells and porcine trophectoderm cells. The expression of small RNAs in these exosomes on days 10 (D10), 13 (D13) and 18 (D18) of pregnancy was comprehensively profiled through sequencing. From nine libraries, a total of 152 known microRNAs (miRNAs), 43 novel miRNAs and 145 novel Piwi-interacting RNAs (piRNAs) were identified. Among these small RNAs, ssc-miR-21-5p, ssc-let-7a, ssc-let-7i-5p and ssc-let-7g were differentially and highly expressed during the three stages. Hierarchical cluster analysis and principal component analysis suggested that the expression profiles of small RNAs in D13 group were different from those in D10 and D18 groups. Bioinformatics analysis showed that the miRNAs differentially expressed in the comparisons between D10 and D13, D13 and D18, and D10 and D18 groups were involved in important molecular functions and pathways such as "G-protein coupled receptor activity" and "Insulin signaling pathway". Conclusions Our results reveal that porcine ULF exosomes contain various small RNAs with potentially vital effects on implantation. This research also provides resources for studies of miRNAs and piRNAs in the cross-talk between embryo and endometrium. BackgroundPrevious studies have indicated that the majority of pre-natal losses occur during the periimplantation period in pigs [1,2]. During this period, the porcine blastocyst expands from 0.5-1 mm in diameter at hatching to 2-mm spheres on day 10 of pregnancy (D10) and begins to rapidly elongate into tubular and filamentous shapes on day 11 or 12 [3,4]. The conceptus starts to attach to the surface of maternal endometrium at day 13 of pregnancy (D13) and the attachment is usually completed between days 18 (D18) and 24 of pregnancy [5,6]. The synchronous communication between conceptus and uterus exerts strong influence on embryo implantation [7]. Furthermore, the

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Exosomes as Theranostic Agents in Reproduction System

Izadpanah,

Yalameha,

Sani

et al. 2023

Advanced Biology

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Exosomes (Exos), belonging to extracellular vesicles, are cell‐derived nano‐sized vesicles with the potential to carry different kinds of biological molecules. Many studies have proved the impacts of exosomal cargo on several biological processes in female and male reproductive systems. It is also hypothesized that changes in exosomal cargo are integral to the promotion of certain pathological conditions, thus Exos can be used as valid biomarkers for the diagnosis of infertility and other abnormal conditions. Here, efforts are made to collect some recent data related to the physiological significance of Exos in the reproductive system, and their potential therapeutic effects. It is anticipated that the current review article will lay the groundwork for elucidating the source and mechanisms by which Exos control the reproductive system additionally supplying fresh methods and concepts for the detection and treatment of disorders associated with fertility for future studies.

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Intrauterine exosomes are required for bovine conceptus implantation

Cited by 83 publications

References 35 publications

Extracellular Vesicles Mediated Early Embryo–Maternal Interactions

Extracellular Vesicles Mediated Early Embryo–Maternal Interactions

Small RNA-seq analysis of exosomes from porcine uterine luminal fluid during peri-implantation

Exosomes as Theranostic Agents in Reproduction System

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