Intratumoural heterogeneity of nuclear DNA-content and proliferation in clear cell type carcinomas of the kidney

Baretton, Gustavo; Kuhlmann, Beatrice; Krech, Rainer; Löhrs, U.

doi:10.1007/bf02890405

Cited by 16 publications

(9 citation statements)

References 20 publications

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“…The results show that an elevated content of p53 is quite frequent in human RCCs, especially in components of clear/chromophilic RCCs and in chromophobic cell tumours. It is important to stress that there was a clear positive correlation between accumulation of p53 protein and the histopathological patterns reflecting relative aggressivity of the tumours [1,4,5,43]. Prognostically favourable oncocytomas and clear cell tumours were mostly p53-negative, whereas more aggressive clear/chromophilic and chromphobic cell tumours frequently displayed p53 immunoreactivity.…”

Section: Discussionmentioning

confidence: 99%

“…Prognostically favourable oncocytomas and clear cell tumours were mostly p53-negative, whereas more aggressive clear/chromophilic and chromphobic cell tumours frequently displayed p53 immunoreactivity. Furthermore, within tumours containing areas of clear and chromophilic cells only chromophilic (acidophilic or granular) areas shown to be more advanced towards malignancy [1,5,8,43] displayed p53-positive immunostaining. These clear-cut findings allow us to exclude any possibility of false-positive immunostaining, which is known to be a common danger in immunohistochemical detection of p53 [15].…”

Section: Discussionmentioning

confidence: 99%

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Elevated content of p53 protein in the absence of p53 gene mutations as a possible prognostic marker for human renal cell tumors

Chemeris

Rempel

Bannasch

et al. 1995

Vichows Archiv A Pathol Anat

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p53 tumour suppressor gene expression was estimated immunohistochemically using DO-1 monoclonal antibody (recognising both wild-type and mutant p53 in 88 human renal tumours. Single strand conformation polymorphism (SSCP) analysis of possible mutations within exons 4-8 of the p53 gene was performed in 29 of the tumours (mostly immunostaining-positive cases). Obviously elevated p53 content was detected with DO-1 antibody in chromophobic cell carcinomas and most clear/chromophilic cell tumours (in chromophilic cell populations). In contrast, clear cell carcinomas demonstrated either complete absence of p53 expression or the presence of single immunopositive nuclei. Oncocytomas were completely negative. Additional immunostaining of the positive samples with mutant p53-specific Pab240 monoclonal antibody failed to detect immunopositive material. No p53 mutation was found in any of the samples analysed by SSCP. Our results suggest that the elevated p53 content in human renal cell carcinomas does not result from gene mutation and the p53 gene alterations are probably not an important mechanism in the development of human renal cell carcinomas. Accumulation of the wild-type p53 protein may be a useful prognostic marker indicating neoplastic progression malignancy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Elevated content of p53 protein in the absence of p53 gene mutations as a possible prognostic marker for human renal cell tumors

Chemeris

Rempel

Bannasch

et al. 1995

Vichows Archiv A Pathol Anat

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“…In contrast, renal tumors with predominantly papillary architecture are characterized cytogenetically by chromosomal trisomies/tetrasomies, most often including chromosomes 7 and 17 and losses of chromosome Y [37,41,56]. Previous molecular studies of RCC have demonstrated a high degree of genetic heterogeneity [3,50,54].…”

Section: Introductionmentioning

confidence: 94%

Genetic progression of renal cell carcinoma

Moch

Mihatsch

2002

Virchows Archiv

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Initiation, progression, and metastasis of cancer are due to genetic alterations. The major challenges of cancer research include the identification of genes involved in metastasis and the evaluation of emerging candidate genes for a potential clinical significance. Renal cancer with its unpredictable metastatic behavior is particularly challenging. The combination of several new molecular technologies, including comparative genomic hybridization, fluorescence in situ hybridization, and cDNA and tissue microarrays have advanced our understanding of renal cancer. However, one usually obtains a limited view of the dynamic process of renal tumor development in a particular cancer patient because renal cell carcinoma is characterized by an accumulation of complex molecular alterations during tumor progression. Some early chromosomal alterations in the carcinogenesis of renal tumors are known, but the nature of subsequent events, their interrelationships, and sequence is poorly understood. To analyze and model cancer development processes, including the presence of multiple pathways, a mathematical method for comparative genomic hybridization data was developed to search for tree models of the oncogenesis process. Tree modeling of comparative genomic hybridization data has provided new information on the interrelationships of genetic changes in renal cancer, their possible order, and a clustering of these events. This review concentrates on the application of comparative genomic hybridization in the area of renal cancer research

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“…The most powerful prognostic parameter in renal cell carcinoma is tumour stage but DNA ploidy and cell kinetic parameters have also been found to be of predictive value (Baretton et al, 1991;Larsson et al, 1993;Ljungberg et al, 1994;Skinner et al, 1971). This tumour type shows a wide spectrum regarding clinical behaviour, from cases with early metastatic presentation to tumours with considerable local growth without invasion or distant manifestations for many years (Skinner et al, 1971.…”

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confidence: 99%

Heterogeneity in renal cell carcinoma and its impact on prognosis - a flow cytometric study

Ljungberg

Mehle²,

Stenling³

et al. 1996

Br J Cancer

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Summary In the process of tumour progression genetic instability is the basis for the evolution of tumour cell clones with various genotypic and phenotypic characteristics causing heterogeneity. Renal cell carcinoma has a long prediagnostic growth period, which increases the probability of clonal evolution. We have studied 200 consecutive renal cell carcinomas, addressing the interrelationship between intratumour heterogeneity and clinicopathological factors. DNA ploidy patterns were analysed in multiple samples from each tumour

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Intratumoural heterogeneity of nuclear DNA-content and proliferation in clear cell type carcinomas of the kidney

Cited by 16 publications

References 20 publications

Elevated content of p53 protein in the absence of p53 gene mutations as a possible prognostic marker for human renal cell tumors

Elevated content of p53 protein in the absence of p53 gene mutations as a possible prognostic marker for human renal cell tumors

Genetic progression of renal cell carcinoma

Heterogeneity in renal cell carcinoma and its impact on prognosis - a flow cytometric study

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