Intratumoral Heterogeneity in DNA Ploidy of Bladder Carcinomas

Sasaki, Kohsuke; Hamano, Katsuhiko; Kinjo, Mitsuru; Hara, Sachiko

doi:10.1159/000227042

Cited by 23 publications

(18 citation statements)

References 8 publications

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“…Absolute counts of immunostaining were made by scoring neoplastic cells, selected among 7–8 non-consecutive fields and chosen in the most viable areas of the lesions (at ×40 magnification) [19,20]. …”

Section: Methodsmentioning

confidence: 99%

Prognostic significance of multidrug-resistance protein (MDR-1) in renal clear cell carcinomas: A five year follow-up analysis

et al. 2006

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Background: A large number of renal cancer patients shows poor or partial response to chemotherapy and the mechanisms have not been still understood. Multi-drug resistance is the principal mechanism by which many cancers develop resistance to chemotherapic drugs. The role of the multi-drug resistant transporter (MDR-1/Pglycoprotein), the gene product of MDR-1, and that one of the so-called multi-drug resistance associated protein (MRP), two energy-dependent efflux pumps, are commonly known to confer drug resistance.

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Section: Methodsmentioning

confidence: 99%

Prognostic significance of multidrug-resistance protein (MDR-1) in renal clear cell carcinomas: A five year follow-up analysis

et al. 2006

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“…Sasaki (20) showed a correlation between more frequent aneuploidy and higher grade of histological malignancy in the group of colorectal carcinomas. However, our results do not confirm this correlation.…”

Section: Discussionmentioning

confidence: 95%

“…Due to such heterogeneity, the cellular ploidy pattern seems to be dependent on the place where tumour material is extracted. Some authors claim that DNA analysis from one segment only is not sufficient (20,23,24). Rognum (15) observed that the more segments of the tumour that were examined, the more frequently aneuploid tumours were recognized.…”

Section: Discussionmentioning

confidence: 99%

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DNA Ploidy in the Treatment of Large Bowel Adenocarcinoma: A Follow up Study

Gawrychowski¹,

Tyczyński²,

Danik³

et al. 2008

Polish Journal of Surgery

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The aim of the study was to look for unfavourable prognostic features in colorectal cancer patients after their surgical treatment as well as to evaluate the prognostic value of cellular ploidy and proliferative activity. Material and methods. A group of 71 colorectal cancer patients discharged from the hospital after surgical treatment in the years 1995-2000 was studied. The examined material was acquired from paraffin blocks of tumour segments. After histopathological verification, the tumour segments recovered from paraffin blocks were used for cytofluorometric analysis of cellular ploidy according to the Hedley method. Results. Diploid tumours were found in 45 of 71 (63.4%) colorectal cancer patients. No significant relationship between DNA ploidy and sex, age, complications, inherited susceptibility to a disease, tumour magnitude, grade of histological malignancy, or grade of clinical progression was observed. After colorectal cancer surgery, the probability of five-and ten-year survival was 44.4% and 37.1%, respectively, for the patients with diploid tumours (DI=1.0), and 38.5% and 9%, respectively, for the patients with aneuploid tumours (DI¹1.0). These differences were not statistically significant (p=0.120). Conclusions. 1. Classical clinicopathologic factors are still the best prognostic criteria for the evaluation of the future results of colorectal cancer patients' surgical treatment. 2. Determination of cellular ploidy and proliferative activity of colonic adenocarcinoma cannot increase the ability to predict prognosis based on surgical treatment . Key words: large bowel adenocarcinoma, DNA ploidy, proliferative activity of large bowel adenocarcinoma Surgical treatment, with possible adjuvant therapy depending on the progression of disease, gives large bowel cancer patients the greatest possibility for recovery (1). However, as the evaluation of clinicopathologic criteria is often subjective and limited, data on prognosis are not objective, and if surgical treatment of large bowel cancer patients is unsuccessful, it is necessary to search for other unknown factors to identify the patients with a risk of a recurrence.The purpose of this study is the following: -to look for unfavourable prognostic features in large bowel cancer patients after their surgical treatment, -to evaluate the prognostic value of cellular ploidy and proliferative activity of large bowel adenocarcinoma.

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“…The time required to develop such heterogeneity varies and must be considerable, and it could be responsible for the different course observed for one same tumor type and tumor stage in different patients [15][16][17] .…”

Section: Discussionmentioning

confidence: 99%

In vivo Aneuploidization during the Expansion of Renal Adenocarcinoma

Sacoto¹,

Alba²,

Alacreu³

et al. 2011

Urol Int

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Background/Aims: A correlation has been observed between DNA ploidy and other prognostic parameters such as tumor stage and grade. The present study evaluates tumor aneuploidization during renal adenocarcinoma expansion and growth. Methods: A total of 252 renal tumors were analyzed between 1969 and 2001. Evaluated variables were age, TNM, Fuhrman classification, histology, size and DNA. A tumor was homogeneous when all the samples were diploid or aneuploid, and a heterogeneous tumor was the coexistence of aneuploid and diploid samples, or all-aneuploid with different aneuploid clones. Results: A total of 224 tumors were included (coefficient of variation <8). The DNA study classified 129 (57.6%) as diploid and 95 (42.4%) as aneuploid. The percentage of aneuploid tumors increased significantly with the pathological stage. Both aneuploid patterns were also significantly more frequent in advanced pathological stages. Tumors with multiple aneuploid clones (n = 17) were significantly more frequent in tumors measuring `4 cm. Both aneuploid patterns showed no differences in survival (p = 0.83), indicating that the heterogeneous pattern probably represents an intermediate step between diploid and homogeneous aneuploid tumor status. Conclusions: The aneuploid pattern is more common in more advanced stages of the disease, with no clear correlation to primary tumor size. This suggests gradual aneuploidization with tumor expansion and growth.

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Intratumoral Heterogeneity in DNA Ploidy of Bladder Carcinomas

Cited by 23 publications

References 8 publications

Prognostic significance of multidrug-resistance protein (MDR-1) in renal clear cell carcinomas: A five year follow-up analysis

Prognostic significance of multidrug-resistance protein (MDR-1) in renal clear cell carcinomas: A five year follow-up analysis

DNA Ploidy in the Treatment of Large Bowel Adenocarcinoma: A Follow up Study

In vivo Aneuploidization during the Expansion of Renal Adenocarcinoma

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