Intratubular penetration of endodontic sealers depends on the fluorophore used for CLSM assessment

Abstract

Root canal filling aims at eliminating empty spaces into the root canal system using biologically compatible materials. Three‐dimensional root canal obturation must prevent or minimize the reinfection caused by microorganisms' leakage. This study aimed at evaluating whether fluorophore (Rhodamine or Fluo‐3) influences the CLSM images of intratubular penetration of four endodontic sealers. Eighty bovine teeth were prepared using K files up to a size #70 and irrigated with 2.5% sodium hypochlorite. All roots were divided into eight groups (n = 10) according to the sealer and fluorophore used: AH Plus/Rhodamine, AH Plus/Fluo‐3, Sealer Plus/Rhodamine, Sealer Plus/Fluo‐3, Sealer Plus BC/Rhodamine, Sealer Plus BC/Fluo‐3, Endosequence/Rhodamine, and Endosequence/Fluo‐3. All roots were filled using cold lateral compaction technique. After 7 days, the roots were transversely sectioned, and three slices, one of each canal third, were obtained. Intratubular penetration was evaluated using CLSM. Sealer Plus BC/Rhodamine and Endosequence BC/Rhodamine presented higher intratubular penetration than AH Plus/Fluo‐3 and Sealer Plus/Fluo‐3 (p ˂ .05). The intragroup analysis showed similar intratubular penetration, regardless of the root third, except for the apical third in AH Plus/Fluo‐3 and Sealer Plus BC/Fluo‐3 groups. The type of fluorophore influences the calcium silicate sealers' tubular penetration but not of epoxy resin‐based ones using CLSM. Bioceramic sealers should not be used associated with Rhodamine for CLSM evaluation. Research Highlights The type of fluorophore influences the calcium silicate sealers' tubular penetration but not of epoxy resin‐based ones when CLSM is used for assessment. Bioceramic sealers should not be used associated with Rhodamine.

Investigation of the effect of different chelation solutions on penetration of resin‐based and bioceramic sealers with a novel method

“…Similar to several previous studies, bioceramic sealers associated with rhodamine B were used in this study (Akcay et al, 2016; El Hachem et al, 2019; Keleş et al, 2018; Sigadam et al, 2020). Furtado et al (2020) stated that bioceramic sealers should not be used associated with rhodamine B, because the water necessary during setting of the sealer may attach to the hydrophilic rhodamine B instead of the bioceramic sealer (Coronas et al, 2020). Therefore, the use of bioceramic sealers associated with rhodamine B may have affected the results of this study.…”

Minimal Dentinal Tubule Penetration of Endodontic Sealers in Warm Vertical Compaction by Direct Detection via SEM Analysis

“…Accordingly, though differences in tubule penetration are reasonable when different techniques are applied, no such effect can be concluded from the available studies. While it was argued that tooth-dependent factors such as variations of the root canal morphology could be the reason for the inconsistency of the studies [ 2 ], the results could also be interpreted as a lack of validation of the investigation technique [ 7 ]. Most studies used rhodamine B dye to stain the sealer.…”

“…No reliable data exist about the penetration depth of endodontic sealers others than from studies where sealers were labelled with rhodamine B [ 7 , 8 ]. Studies using a calcium marker indicated that depth of apatite crystal precipitation in the dentinal tubules is more inferior than expected from previous studies [ 4 , 7 , 9 ].…”

Effect of Gallic Acid on the Filling Quality of an Epoxy Resin – Based Root Canal Sealer

“…In this study, the obturation quality was assessed by using CLSM which have sereral advantages over (SEM) such as being simpler, easy and nondestructive sample processing, as well as lower tendency to produce artefacts [25] . Moreover the imags obtained by CLSM allows a quantitative data, unlike the qualitative scores commonly used in SEM imags, which may provide less reliability [26]. Rhodamine B dye is the standard fluorophore dye used, and it has been previously demonstrated that your use of in low concentrations (0.1%) did not change the sealer properties, especially with epoxy resin-based sealer [6,27] .…”