Intrathoracic esophageal replacement by in situ tissue-engineered esophagus

Abstract

The in situ tissue-engineered esophagus can successfully replace the intrathoracic esophagus, and this procedure may offer a promising surgical approach to esophageal diseases.

Analysis of the structure and neuritogenic activity of chondroitin sulfate/dermatan sulfate hybrid chains from porcine fetal membranes

“…The unusually flexible ring conformation of IdoA residues in FM-CS/DS may be involved in the biological activities of AM such as promotion of the proliferation of corneal epithelium [18] and regeneration of esophagus and skin [37,38] Since normal cornea abundantly contains collagen fibrils, the markedly large size of FM-CS/DS may contribute to the treatment of corneal diseases.…”

“…Consecutive IdoA residues appear to be 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 21 AM can improve the morphogenesis of epidermis and basement membranes in constructing a human living skin-equivalent [38]. The in situ tissue-engineered esophagus, which is prepared by cultivating oral keratinocytes and fibroblasts on human AM, can successfully replace the intrathoracic esophagus [37]. Since CS/DS is involved in biological events such as cell proliferation [3,6], viral infection [46], inflammation [47], anti-coagulation [33], and wound healing [5,6,48], it may contribute to some of the biological activities of AM described above.…”

Bioengineering of Colo-Rectal Tissue

“…While even long-term culture of colon epithelial cells were successfully established, their use for tissue engineering purposes have not been published up to date. Given that in vitro seeding of different types of epithelial cells on a scaffold was reported to improve integration of the implanted biomaterial in vivo (Nakase et al, 2008;Oberpenning et al, 1999;Yoo et al, 1998), we speculate that this might also apply to colonic epithelial cells. Furthermore, cell-populated biomaterials may be less prone to inflammation with subsequent fibrosis and scaffold shrinkage due to the fact that the commensal flora is prevented to get into contact with the biomaterial itself.…”

Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells

“…Successful regeneration was limited to the epithelium, submucosa, and required at least a partial native esophageal muscular layer without which strictures developed (Badylak et al 2005). Another report describes PGA scaffolds loaded with oral keratinocytes and fibroblasts successfully transplanted into a canine esophageal resection (Nakase et al 2008). However, this segment remained aperistaltic.…”