Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells

Trecartin, Andrew; Grikscheit, Tracy C.

doi:10.1101/cshperspect.a025700

Cited by 6 publications

(5 citation statements)

References 140 publications

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“…(), and work on the regional identity of OUs/enteroids has so far indicated that the source region is correlated with aspects of the subsequent cellular or tissue growth. For donor regions of the gastrointestinal tract resulting in the growth of tissue‐engineered intestine, and in fact for other organs, such as lung (Sala et al., ; Speer et al., ; Spurrier, Speer, Hou, El‐Nachef, & Grikscheit, ; Trecartin & Grikscheit, ; Trecartin et al., ), regional identity is maintained; for example, growing regions of the stomach, antrum versus whole stomach (Speer, Sala, Matthews, & Grikscheit, ) results in growth only of the harvested and transplanted region.…”

Section: Discussionmentioning

confidence: 99%

Short‐term and long‐term human or mouse organoid units generate tissue‐engineered small intestine without added signalling molecules

Hou

Chang

Trecartin

et al. 2018

Experimental Physiology

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Mouse and human postnatal and fetal organoid units (OUs) maintained in either short-term culture (2 weeks) or long-term culture (from 4 weeks up to 3 months) without adding exogenous growth factors were implanted in immunocompromised mice to form tissue-engineered small intestine (TESI) in vivo. Intestinal epithelial stem and neuronal progenitor cells were maintained in long-term OU cultures from both humans and mice without exogenous growth factors, and these cultures were successfully used to form TESI. This was enhanced with OUs derived from human fetal tissues. Organoid unit culture is different from enteroid culture, which is limited to epithelial cell growth and requires supplementation with R-Spondin, noggin and epidermal growth factor. Organoid units contain multiple cell types, including epithelial, mesenchymal and enteric nervous system cells. Short- and long-term cultured OUs derived from mouse and human intestine develop into TESI in vivo, which contains key components of the small intestine similar to native intestine.

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Section: Discussionmentioning

confidence: 99%

Short‐term and long‐term human or mouse organoid units generate tissue‐engineered small intestine without added signalling molecules

Hou

Chang

Trecartin

et al. 2018

Experimental Physiology

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“…While direct transplantation of ENCCs is a promising approach for cell replacement therapy for HSCR, the emergence of colonic organoid technology may allow the creation of an artificial colon for engraftment into the patient, representing an alternative treatment for HSCR. Different cell lineages within the 3-D organoids can self-organize to produce cytoarchitecture resembling the in vivo tissues, so it should be feasible to use organoids to build an artificial organ for replacement therapy [ 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ]. It has been demonstrated that the transplanted colonic organoids can grow and be maintained for a long period of time in vivo after engrafting into the colon epithelium [ 60 ], suggesting that the ENS-innervated colonic organoids also have the potential to functionally integrate with the endogenous colon of the HSCR patients.…”

Section: Hpsc-based Replacement Therapy For Hscrmentioning

confidence: 99%

Human Pluripotent Stem Cell-Based Models for Hirschsprung Disease: From 2-D Cell to 3-D Organoid Model

Lui

Ngan

2022

Cells

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Hirschsprung disease (HSCR) is a complex congenital disorder caused by defects in the development of the enteric nervous system (ENS). It is attributed to failures of the enteric neural crest stem cells (ENCCs) to proliferate, differentiate and/or migrate, leading to the absence of enteric neurons in the distal colon, resulting in colonic motility dysfunction. Due to the oligogenic nature of the disease, some HSCR conditions could not be phenocopied in animal models. Building the patient-based disease model using human induced pluripotent stem cells (hPSC) has opened up a new opportunity to untangle the unknowns of the disease. The expanding armamentarium of hPSC-based therapies provides needed new tools for developing cell-replacement therapy for HSCR. Here we summarize the recent studies of hPSC-based models of ENS in 2-D and 3-D culture systems. These studies have highlighted how hPSC-based models complement the population-based genetic screens and bioinformatic approaches for the discovery of new HSCR susceptibility genes and provide a human model for the close-to-physiological functional studies. We will also discuss the potential applications of these hPSC-based models in translational medicines and their advantages and limitations. The use of these hPSC-based models for drug discovery or cell replacement therapy likely leads to new treatment strategies for HSCR in the future. Further improvements in incorporating hPSC-based models with the human-mouse chimera model and organ-on-a-chip system for establishing a better disease model of HSCR and for drug discovery will further propel us to success in the development of an efficacious treatment for HSCR.

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“…Intestinal progenitor stem cells are intensively used for tissue engineering of the stomach, small intestine, and bowel in combination with polymer scaffolds[49,50]. However, investigation of intestinal stem cells on scaffolds from PHAs or even synthetic polyhydroxyalkanoates or the development of tissue-engineered devices based on PHA scaffolds seeded with intestinal stem cells are very rare.…”

Section: Overview Of the Effect Of Phas On Msc Growth And Differentiamentioning

confidence: 99%

Effect of poly(3-hydroxyalkanoates) as natural polymers on mesenchymal stem cells

Voinova

Бонарцева

Бонарцев

2019

WJSC

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Mesenchymal stem cells (MSCs) are stromal multipotent stem cells that can differentiate into multiple cell types, including fibroblasts, osteoblasts, chondrocytes, adipocytes, and myoblasts, thus allowing them to contribute to the regeneration of various tissues, especially bone tissue. MSCs are now considered one of the most promising cell types in the field of tissue engineering. Traditional petri dish-based culture of MSCs generate heterogeneity, which leads to inconsistent efficacy of MSC applications. Biodegradable and biocompatible polymers, poly(3-hydroxyalkanoates) (PHAs), are actively used for the manufacture of scaffolds that serve as carriers for MSC growth. The growth and differentiation of MSCs grown on PHA scaffolds depend on the physicochemical properties of the polymers, the 3D and surface microstructure of the scaffolds, and the biological activity of PHAs, which was discovered in a series of investigations. The mechanisms of the biological activity of PHAs in relation to MSCs remain insufficiently studied. We suggest that this effect on MSCs could be associated with the natural properties of bacteria-derived PHAs, especially the most widespread representative poly(3-hydroxybutyrate) (PHB). This biopolymer is present in the bacteria of mammalian microbiota, whereas endogenous poly(3-hydroxybutyrate) is found in mammalian tissues. The possible association of PHA effects on MSCs with various biological functions of poly(3-hydroxybutyrate) in bacteria and eukaryotes, including in humans, is discussed in this paper.

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Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells

Cited by 6 publications

References 140 publications

Short‐term and long‐term human or mouse organoid units generate tissue‐engineered small intestine without added signalling molecules

Short‐term and long‐term human or mouse organoid units generate tissue‐engineered small intestine without added signalling molecules

Human Pluripotent Stem Cell-Based Models for Hirschsprung Disease: From 2-D Cell to 3-D Organoid Model

Effect of poly(3-hydroxyalkanoates) as natural polymers on mesenchymal stem cells

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