Intrathecal synthesis of specific immunoglobulin G antibodies in neurocysticercosis: evaluation of antibody concentrations by enzyme-linked immunosorbent assay using a whole cysticercal extract and cyst vesicular fluid as antigens

Arruda, Gisele Cristina; Quagliato, Elizabeth Maria Aparecida Barasnevicius; Rossi, Claudio

doi:10.1016/j.diagmicrobio.2005.08.007

Cited by 6 publications

(6 citation statements)

References 25 publications

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“…However, there was no significant change in mice immunized via the SC route, which suggests IT synthesis to be the most likely source of the excess immunoglobulin. Antibody production in the CSF has been reported in normal brain [6] as well as in animals and humans with increased blood-brain barrier permeability or other pathologies of the CNS [1,10,[34][35][36]. Immunologic processing with drainage and retention of brain-derived antigens is an important factor in the initiation and regulation of immune responses in the CNS.…”

Section: Discussionmentioning

confidence: 99%

Effective Prevention against Rabies by Intracerebral Immunization in Mice

Shin

Sakoda

Yano

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. To evaluate the efficacy of intracerebral (IC) immunization, mice were immunized with a rabies vaccine by the subcutaneous (SC), intramuscular (IM) or IC route, and 10-fold the 50% lethal dose of rabies virus was inoculated into the hindleg of the immunized or non-immunized mice. The antibody titer in serum was elevated and boosted by additional immunization via all routes, but highest after the IC immunization followed by the IM and SC routes, in this order. Intracerebrally immunized mice were completely protected from death and the neurological signs of infection, whereas the IM or SC immunization only partly protected the mice. In mouse models, IC immunization is more effective at inducing a protective immune response against the transneural spread of rabies virus than IM or SC immunization.

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Section: Discussionmentioning

confidence: 99%

Effective Prevention against Rabies by Intracerebral Immunization in Mice

Shin

Sakoda

Yano

et al. 2009

The Journal of Veterinary Medical Science

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“…The elevated CSF antibody titers detected only in SA immunized and not in SC immunized rabbits suggest IT synthesis as the most likely source of the excess immunoglobulin. Antibody production in the CSF has been reported in normal brain (8) as well as in animals and humans with increased blood-brain barrier permeability or other pathologies of the CNS (3,12,(43)(44)(45). Immunologic processing with drainage and retention of brain-derived antigens is an important factor in the initiation and regulation of immune responses in the CNS.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Intracerebral Immunization against Pseudorabies Virus in Mice

Shin

Sakoda

Kim

et al. 2006

Microbiology and Immunology

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Abstract:To evaluate the efficacy of intracerebral (IC) immunization, mice were immunized with formalininactivated pseudorabies virus (PRV) by either subcutaneous (SC) or IC injection, and then 10 6 plaqueforming units of PRV were introduced into the hindleg of the immunized or non-immunized mice by intramuscular injection. The antibody titer in serum was elevated and boosted by additional immunization via both the SC and IC routes, but was higher after IC immunization. Intracerebrally immunized mice were completely protected from mortality and neurological signs, whereas all the non-immunized and 80% of the subcutaneously immunized mice died after developing neurological signs. In mouse models, IC immunization is more effective at inducing a protective immune response against the transneural spread of PRV than SC immunization.

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“…In the present study, all patients with neurocysticercosis had neuroimaging findings compatible with neurocysticercosis and specific IgG antibody index indicative of intrathecal antibody production. The demonstration of intrathecal antibody production has proven useful for showing the involvement of the CNS in several diseases, including cysticercosis 11,28 . The detection of intrathecal antibody synthesis allows the immunologic diagnosis of the disease even when inflammatory alterations are not found in the CSF 28 .…”

Section: Referencesmentioning

confidence: 99%

“…All of the patients with neurocysticercosis included in this study had intrathecal synthesis of cysticercus-specific IgG antibodies. The detection of intrathecal antibody production was based on calculation of the specific IgG antibody index, as previously described 11 . All of the patients were attended at the University Hospital of the State University of Campinas (UNICAMP).…”

Section: Csf Samplesmentioning

confidence: 99%

Evaluation of cysticercus-specific IgG (total and subclasses) and IgE antibody responses in cerebrospinal fluid samples from patients with neurocysticercosis showing intrathecal production of specific IgG antibodies

Suzuki

Rossi

2013

Arq. Neuro-Psiquiatr.

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In the present study, an enzyme-linked immunosorbent assay (ELISA) standardized with vesicular fluid of Taenia solium cysticerci was used to screen for IgG (total and subclasses) and IgE antibodies in cerebrospinal fluid (CSF) samples from patients with neurocysticercosis showing intrathecal production of specific IgG antibodies and patients with other neurological disorders. The following results were obtained: IgG-ELISA: 100% sensitivity (median of the ELISA absorbances (MEA)=1.17) and 100% specificity; IgG 1 -ELI-SA: 72.7% sensitivity (MEA=0.49) and 100% specificity; IgG 2 -ELISA: 81.8% sensitivity (MEA=0.46) and 100% specificity; IgG 3 -ELISA: 63.6% sensitivity (MEA=0.12) and 100% specificity; IgG 4 -ELISA: 90.9% sensitivity (MEA=0.85) and 100% specificity; IgE-ELISA 93.8% sensitivity (MEA=0.60) and 100% specificity. There were no significant differences between the sensitivities and specificities in the detection of IgG-ELISA and IgE-ELISA, although in CSF samples from patients with neurocysticercosis the MEA of the IgG-ELISA was significantly higher than that of the IgE-ELISA. The sensitivity and MEA values of the IgG 4 -ELISA were higher than the corresponding values for the other IgG subclasses. Future studies should address the contribution of IgG 4 and IgE antibodies to the physiopathology of neurocysticercosis.Key words: IgG, IgE, enzyme-linked immunosorbent assay, neurocysticercosis. RESUMONo presente estudo, uma reação imunoenzimática (ELISA) padronizada com o fluido vesicular de cisticercos de Taenia solium foi utilizada para avaliar as respostas de anticorpos anti-cisticercos IgG (total e subclasses) e IgE em amostras de líquido cefalorraquidiano (LCR) de pacientes com neurocisticercose apresentando produção intratecal de anticorpos específicos IgG e pacientes com outras desordens neurológicas. Os seguintes resultados foram obtidos: ELISA-IgG: 100% de sensibilidade (mediana das absorbâncias das reações ELISA (MAE)=1,17) e especificidade 100%; ELISA-IgG 1 : sensibilidade 72,7% (MAE=0,49) e especificidade 100%; ELISA-IgG 2 : sensibilidade 81,8% (MAE=0,46) e especificidade 100%; ELISA-IgG 3 : sensibilidade 63,6% (MAE=0,12) e especificidade 100%; ELISA-IgG 4 : sensibilidade 90,9% (MAE=0,85) e especificidade 100%; ELISA-IgE: sensibilidade 93,8% (MAE=0,60) e especificidade 100%. Não foram encontradas diferenças significativas entre as sensibilidades e especificidades das reações ELISA-IgG e ELISA-IgE, embora a MAE da reação ELISA-IgG em amostras de LCR de pacientes com neurocisticercose tenha sido significativamente maior que a obtida com ELISA-IgE. Os valores de sensibilidade e MAE da reação ELISA-IgG 4 foram maiores que os valores correspondentes para as outras subclasses da IgG. Estudos futuros deverão abordar a contribuição dos anticorpos IgG 4 e IgE na fisiopatologia da neurocisticercose.Palavras-Chave: IgG, IgE, ensaio de imunoadsorção enzimática, neurocisticercose.

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Intrathecal synthesis of specific immunoglobulin G antibodies in neurocysticercosis: evaluation of antibody concentrations by enzyme-linked immunosorbent assay using a whole cysticercal extract and cyst vesicular fluid as antigens

Cited by 6 publications

References 25 publications

Effective Prevention against Rabies by Intracerebral Immunization in Mice

Effective Prevention against Rabies by Intracerebral Immunization in Mice

Efficacy of Intracerebral Immunization against Pseudorabies Virus in Mice

Evaluation of cysticercus-specific IgG (total and subclasses) and IgE antibody responses in cerebrospinal fluid samples from patients with neurocysticercosis showing intrathecal production of specific IgG antibodies

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