Intraspecific Variation in Cannabis sativa L. Based on Intergenic Spacer Region of Chloroplast DNA.

Kohjyouma, Mareshige; Lee, I-Jung; Iida, Osamu; Kurihara, Kogo; Yamada, Kazuya; Makino, Yasutaka; Sekita, Setsuko; Satake, Motoyoshi

doi:10.1248/bpb.23.727

Cited by 35 publications

(22 citation statements)

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“…Within this region, only limited nucleotide polymorphisms was detected; this is not surprising, as it is known that chloroplast DNA has both a very low structural evolution and nucleotide synonymous substitution rate. In fact, among the entries present in NCBI for this sequences, 34 are actually the same sequence obtained from different Cannabis sources, by different authors, and are practically coincident, with only a nucleotide missing in one sequence compared to the others (Kohjyouma et al, 2000).…”

Section: The Forensic Applications Of Dna Markersmentioning

confidence: 87%

Potential of marker-assisted selection in hemp genetic improvement

Mandolino

Carboni

2004

Euphytica

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The development and applications of molecular markers to hemp breeding are recent, dating back only to the mid1990s. The main achievements in this field are reviewed. The analysis of Cannabis germplasm by RAPD, AFLP and microsatellites is discussed, with its consequence for the still debated species concept in Cannabis. DNA-based markers have also been exploited in the field of forensic science, in an attempt to discriminate licit from illicit crop. The main applications of the molecular markers to the breeding, however, have been achieved with the development of markers closely linked to the male sex and to some of the most relevant chemotypes. Active research is carried out by several groups in the field of identification and characterization of the genes involved in fiber quality and quantity, and in the determination of monoecy, another very important target of hemp breeding. Besides, markers associated to new, potentially useful chemotypes are being developed, for the marker-assisted breeding of pharmaceutical Cannabis.

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Section: The Forensic Applications Of Dna Markersmentioning

confidence: 87%

Potential of marker-assisted selection in hemp genetic improvement

Mandolino

Carboni

2004

Euphytica

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“…sativa is a dioecious and obligate outbred species with a complex genetic constitution and heredity. The genetic complexity may explain the phenotype variability, sex expression, polymorphism, and the substantial biological plasticity of this species (Schultes et al 1974;Kohiyouma et al 2000). Dioecy is the ancestral state of family Cannabaceae, and members of this family may have one of the oldest sex chromosome systems ).…”

Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic analysis of monoecious hemp (Cannabis sativa L.) cultivars reveals its karyotype variations and sex chromosomes constitution

et al. 2015

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Hemp (Cannabis sativa L., 2n = 20) is a dioecious plant. Sex expression is controlled by an X-to-autosome balance system consisting of the heteromorphic sex chromosomes XY for males and XX for females. Genetically monoecious hemp offers several agronomic advantages compared to the dioecious cultivars that are widely used in hemp cultivation. The male or female origin of monoecious maternal plants is unknown. Additionally, the sex chromosome composition of monoecious hemp forms remains unknown. In this study, we examine the sex chromosome makeup in monoecious hemp using a cytogenetic approach. Eight monoecious and two dioecious cultivars were used. The DNA of 210 monoecious plants was used for PCR analysis with the male-associated markers MADC2 and SCAR323. All monoecious plants showed female amplification patterns. Fluorescence in situ hybridization (FISH) with the subtelomeric CS-1 probe to chromosomes plates and karyotyping revealed a lack of Y chromosome and presence of XX sex chromosomes in monoecious cultivars with the chromosome number 2n = 20. There was a high level of intra- and intercultivar karyotype variation detected. The results of this study can be used for further analysis of the genetic basis of sex expression in plants.

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“…Prentice et al (2003) implied chloroplast universal primers to study variation in cultivated and wild Prunus avium. Other examples for the use of universal primers to assess intraspecific variation are those carried out on Argania spinosa (Sapotaceae) of Morocco (El Mousadik and Petit 1996), Primula cuneifolia (Primulaceae) (Fujii et al 1999), Cannabis sativa (Kohjyouma et al 2000), the rare Spanish endemic, Silene hifacensis (Prentice et al 2003), Sagittaria latifolia (Dorken and Barrett 2004), and Oryza sativa (Ou et al 2009). …”

Section: Spontaneous Hybridisationmentioning

confidence: 99%

Chloroplast-specific universal primers and their uses in plant studies

Haider¹

2011

Biologia plant.

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Universal (consensus) primers are those primers that have the ability to amplify the targeted region of DNA across a broad range of individuals in a certain group of organisms. In plants, such universal primers have been designed to target regions in the nuclear, mitochondrial or chloroplast genome. Among these three genomes, the chloroplast genome is the most suited for the design of consensus primers due to the lower rate of evolution and hence conservation of gene order and sequence of the genome among the different plant species compared to the other two genomes. Several molecular studies in plants have developed and used chloroplast-specific universal primers. In this review, I present some examples of the nuclear DNA-specific universal primers and discuss the features of the chloroplast DNA that make it the most suited for the design of such primers. I then refer to all chloroplast-specific primers developed so far and provide some examples of molecular studies and applications that made use of them.

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Intraspecific Variation in Cannabis sativa L. Based on Intergenic Spacer Region of Chloroplast DNA.

Cited by 35 publications

References 1 publication

Potential of marker-assisted selection in hemp genetic improvement

Potential of marker-assisted selection in hemp genetic improvement

Molecular cytogenetic analysis of monoecious hemp (Cannabis sativa L.) cultivars reveals its karyotype variations and sex chromosomes constitution

Chloroplast-specific universal primers and their uses in plant studies

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