Intraspecific Variability of Biologically Active Compounds of Different Populations ofTribulus TerrestrisL. (Zygophyllaceae) in South Bulgaria

Lazarova, Irına; Ivanova, Alexandra; Mechkarova, Pepa; Peev, Dimitar; Valyovska, Natalia

doi:10.5504/bbeq.2011.0040

Cited by 8 publications

(13 citation statements)

References 6 publications

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“…Указывается также, что способ экстракции стероидных сапонинов, очевидно, влияет на результаты их определения в сырье якорцев [45]. Поэтому данные об отсутствии, например, диосцина в некоторых анализировавшихся другими исследовательскими группами образцах [48] позволяют предположить, что фаза развития растений якорцев стелющихся и конкретная методика экстракции стероидных сапонинов оказывают существенное влияние на результат.…”

Section: Discussionunclassified

“…В научной литературе, посвящённой исследованию травы якорцев стелющихся, представлены сведения о различных методах, использовавшихся для оценки содержания стероидных сапонинов, в том числе диосцина и протодиосцина, в этом лекарственном растительном сырье [46,48].…”

Section: Discussionunclassified

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Phytochemical study of Tribulus Terrestris L.

Аффуф

Карпенко

Гуляев

et al. 2020

Farm. farmakol. (Pâtigorsk)

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Tríbulus terrestris L., an annual herb belonging to the Zygophyllaceae family and growing in moderate and tropical climates, has a rich chemical composition of biologically active substances and chemical elements.The aim of the work is a phytochemical study of Tribulus terrestris L. growing in different geographical zones.Materials and methods. The objects of study were herb specimens of Tribulus terrestris L. collected in different habitats. The samples of the raw materials were shade-dried. The determination of saponins in the raw materials, was carried out by high performance liquid chromatography with a mass spectrometric detection (HPLC-MS / MS). The study of the qualitative and quantitative composition of the elements was carried out on an X-ray fluorescence spectrometer.Results. The saponins had been studied by HPLC-MS/MS, according to which in all the studied samples, dioscin and protodioscin were found. Their retention times coincided with the retention times of dioscin and protodioscin standards. It has been established that among the macroelements of Tríbulus terrestris L., potassium and calcium are mostly accumulated. They account for about 90% of the total content of the elements in the plant. It has been revealed that the distribution of macroand microelements in the plant, varies significantly depending on their place and growing conditions.Conclusion. The maximum dioscin content was observed in the samples harvested in Moldova, and the minimum – in the samples from the nursery garden of the All-Russian Scientific Research Institute of medicinal and aromatic plants. The largest amount of protodioscin was found out in the samples from the Crimea, and the minimum – in the samples from Moldova. The carried out study of the elements content of Tríbulus terrestris L. showed that the habitats (geographical zones) in which the studied samples of raw materials had been were collected, affect the accumulation of the elements by the plant. Based on the data obtained, biological absorption series have been compiled for the samples from each habitat.

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Section: Discussionunclassified

Phytochemical study of Tribulus Terrestris L.

Аффуф

Карпенко

Гуляев

et al. 2020

Farm. farmakol. (Pâtigorsk)

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“…15 Leaves had the highest steroid saponin content, followed by fruits and stems. 15 For the Bulgarian samples, the saponin accumulation was highest in the flowering stage, and 38 Astragalin (kaempferol-3-glucoside) Aboveground part Saleh et al 93 Flavonols; kaempferol; quercetin; isorhamnetin; 3-gentiobiosides Whole plants Zafar and Nasa 44 Kaempferol; quercetin Fruits, stem Louveaux et al 94 18 83 Rutin Fruits/leaves (1:1) Ivanova et al 84 Rutin Rutin Fruits/leaves (1:1) Lazarova et al 85 Rutin Fruits/leaves (1:1) Navanath et al 97 Phenolics; flavonoids Fruits Noori et al 98 Chrysin; flavone C and C-/O-glycosides; flavonoid sulfates; aglycones in addition to flavone C and C-/O-glycosides…”

Section: Presence Of Chemoraces According To Geographical Originmentioning

confidence: 99%

“…Some studies examined the quantitative variability in the plant's basic saponins: PD, PT, dioscin, and the flavonoid glucoside rutin. [83][84][85]105 These active components are specific for the saponin profile of the Bulgarian populations of TT. 83 It was established that the quantity of PD, PT, dioscin, and rutin increases in TT populations of southern Bulgaria, contrary to the populations of northern Bulgaria.…”

Section: Stem Leaves Fruitsmentioning

confidence: 99%

“…Samples have been collected from different floral regions in Bulgaria in different climatic conditions. [83][84][85] Populations located in the southern part of the country may have been influenced by higher temperatures and had higher rutin concentrations. 84 PD, PT, and rutin dominated mainly in plant populations found in the micro region of Haskovo Plain (the towns of Simeonovgrad, Balgarin, Lyubimets, G. Dobrevo, and Raykova Mogila), as well as the region of Pazardzhik-Plovdiv Plain (the towns of Asenovgrad and Pazardzhik).…”

Section: Flavonoidsmentioning

confidence: 99%

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Chemical Constituents, Biological Properties, and Uses of Tribulus terrestris: A Review

Semerdjieva

Zheljazkov

2019

Natural Product Communications

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Tribulus terrestris L. (TT) (puncturevine) is a common weed that grows in many countries worldwide and in some places is considered as a noxious weed. The plant has been used in traditional Chinese and Indian medicines and is now considered as one of the most popular aphrodisiacs. It is known for its healing properties for sexual difficulties, impotence, and human and animal hormonal imbalance. It is also used as a sexual booster. Because of the plant’s active substances that can be used for curing sexual and other disorders, interest in it is increasing, and it is currently one of the most studied medicinal plants. The products and preparations manufactured from the aboveground plant parts are especially popular among athletes and people with health issues and diseases such as hormonal imbalance, sexual problems, heart problems, and various kidney and skin diseases. The aim of this review is a comprehensive and critical assessment of the scientific publications involving TT, with special reference to its chemical constituents and biological properties that may facilitate current understanding and future studies of this fascinating plant species. The objectives of this review were (1) to find knowledge gaps, (2) to discuss critically relevant publications and issues with materials and methods that may be prerequisites for contradictory results, and (3) to identify research and development areas. It was found that some of the studies on TT extracts as aphrodisiacs are controversial. A significant number of research publications claim that TT extracts and nutritional supplements containing TT improve muscle tone, have a common biostimulating effect, and improve spermogenesis. However, there are a growing number of publications that dispute these claims, as there are no empirical data on commonly accepted mechanisms of action. The main biologically active substances in TT are steroidal saponins, flavonoids, alkaloids, and lignan amides, the most studied being the steroidal saponins. Multiyear data on the metabolic profile of the species are generally lacking. There are a variety of methods used for extracting plant material, differences in methodologies and saponin analyses, and scientific instruments that were used. Lack of common standards could be a reason for differences in the pharmacological activity and composition of the TT preparations. Development of standard procedures and methods for collection of plant material and analyses are recommended. Selection and breeding efforts and agronomic studies of promising clones of TT would need to be conducted in order to develop TT as a new crop. This will provide consistency of supply and quality of the feedstock for the pharmaceutical industry and could provide a new cash crop for growers.

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Development and Validation of a UV Spectrophotometric Method for the Determination of Dioscin in Tribulmon Tablets

Dovchinsuren

Dorjbal

2021

Cent. Asian j. Med. Sci.

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Objective: Develop and validate a novel, sensitive, precise and straightforward UV spectrophotometric method to determine the amount of dioscin in tribulmon tablets. Methods: tribulmon tablets were prepared in the laboratory from medicinal plants Tribulus Terrestris L, native to Mongolia. Extract solutions were prepared from a single batch of tribulmon tablets, and the amount of dioscin was determined by UV spectrophotometric method using a calibration curve of known solutions of the dioscin. A chromogenic reaction was used to determine the amount of dioscin in the tablets. The maximum absorption was 201 nm. Validation parameters were evaluated following ICH guidelines. Results: The correlation coefficient of linearity for the UV spectrophotometric method was 0.9996. The standard working solution was linear from 3.2 to 18.9 μg/mL. The Limit of Detection (LOD) and Limit of Quantitation (LOQ) were 1.9 μg/ mL and 5.9 μg/mL, respectively. The recovery test accuracy was ranged from 97.9 to 100%. The %RSD for repeatability and intra-day precision was 0.45%, 0.13-0.50%, respectively. The %RSD for robustness was 0.26% to 0.40%, respectively. Conclusion: Based on the results of the proposed UV spectrophotometric method, the method we developed to determine dioscin content in tribulmon tablets from Tribulus Terrestris L is sensitive, precise, stable and straightforward. The test's validation parameters comply with ICH requirements.

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Intraspecific Variability of Biologically Active Compounds of Different Populations ofTribulus TerrestrisL. (Zygophyllaceae) in South Bulgaria

Cited by 8 publications

References 6 publications

Phytochemical study of <i>Tribulus Terrestris</i> L.

Phytochemical study of <i>Tribulus Terrestris</i> L.

Chemical Constituents, Biological Properties, and Uses of Tribulus terrestris: A Review

Development and Validation of a UV Spectrophotometric Method for the Determination of Dioscin in Tribulmon Tablets

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