Intraspecific Genetic Diversity of Two Black Fly Species (Diptera: Simuliidae) from South India using DNA Barcode Based RFLP Analysis

Abstract

The population genetic diversity of two black fly species (S. (S.) gurneyae and S. (S.) nilgiricum) was investigated along an elevational gradient in two hills of South India by mitochondrial gene sequence of cytochrome coxidase subunit I (COI) based Restriction Fragment Length Polymorphism (RFLP). By comparing the similarity of the bands produced by three restriction endonuclease enzymes, the genetic distance was calculated among sampling sites. The mean Kimura two parameter divergences of two black fly species between sampling sites was observed less than 13% and the higher genetic differentiation occurred at higher elevational sites (>1000 m). These result suggest that hypothesis of speciation is related with higher elevational site in black flies.

Genetic Homogenity of Commerson’s Anchovy (Stolephorus commersonnii) in Segara Anakan Cilacap Central Java Inferred from PCR-RFLP Markers

“…This result indicated that only one restriction site on the COI gene of S. commersonnii was recognized by that enzyme. Our result similar to previous study from Arunprasanna et al (2015) on Simulium gurneyae and Simulium nilgirium although with different sizes (468 bp and 241 bp). This similarity indicates that the same partial sequences of COI gene in different species has similar restriction site for VspI although amplified from different species.…”