Triplet excited states exhibit strongly medium-dependent properties, which turns them into valuable tools to probe the microenvironments existing in protein binding Finally, protein encapsulation can also modify the photoreactivity of the guest. This is illustrated by presenting an example of retarded photooxidation.
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Graphical abstractKeywords: 1-acid glycoproteins, drug, laser flash photolysis, serum albumins, protein binding, transient absorption spectroscopy.